Alignments for a candidate for argB in Saccharomonospora cyanea NA-134

GapMind for Amino acid biosynthesis

Alignments for a candidate for argB in Saccharomonospora cyanea NA-134

Align acetylglutamate kinase (EC 2.7.2.8) (characterized)
to candidate WP_005457887.1 SACCYDRAFT_RS16645 acetylglutamate kinase

Query= BRENDA::Q6R6P5
         (317 letters)



>NCBI__GCF_000244975.1:WP_005457887.1
          Length = 309

 Score =  407 bits (1046), Expect = e-118
 Identities = 194/282 (68%), Positives = 239/282 (84%)

Query: 13  RANVLAEALPWLQHFRDKIVVVKYGGNAMVDDDLKAAFAADMVFLRTVGAKPVVVHGGGP 72
           +A+VL EALPWLQ F    VVVKYGGNAMVDD+LK AFA DMVFLR  G +PVVVHGGGP
Sbjct: 20  KASVLIEALPWLQRFHGATVVVKYGGNAMVDDELKRAFAQDMVFLRIAGLRPVVVHGGGP 79

Query: 73  QISEMLNRVGLQGEFKGGFRVTTPEVMDIVRMVLFGQVGRDLVGLINSHGPYAVGTSGED 132
           QI+ ML+R+G++GEFKGG RVTTPE MD+VRMVL GQV R+LVGLIN+HGPYAVG SGED
Sbjct: 80  QITAMLDRLGIKGEFKGGLRVTTPETMDVVRMVLVGQVSRELVGLINAHGPYAVGISGED 139

Query: 133 AGLFTAQKRMVNIDGVPTDIGLVGDIINVDASSLMDIIEAGRIPVVSTIAPGEDGQIYNI 192
           A LFTA+++   +DG P D+GLVG++++V+  +++DI+ AGRIPVVST+AP  DG ++N+
Sbjct: 140 ARLFTAERKQATVDGEPVDVGLVGEVVDVNPDAVLDIVNAGRIPVVSTVAPDTDGVVHNV 199

Query: 193 NADTAAGALAAAIGAERLLVLTNVEGLYTDWPDKSSLVSKIKATELEAILPGLDSGMIPK 252
           NADTAAGALA+A+ AE+L+VLT+VEGLY +WPD+SSLV +I A ELE +LP L SGMIPK
Sbjct: 200 NADTAAGALASALRAEKLVVLTDVEGLYANWPDRSSLVDRIDADELENLLPSLASGMIPK 259

Query: 253 MESCLNAVRGGVSAAHVIDGRIAHSVLLELLTMGGIGTMVLP 294
           ME+CL A+RGGV  AHVIDGR+AHSVLLE+ T  G+GTMV+P
Sbjct: 260 MEACLRAIRGGVRRAHVIDGRLAHSVLLEVFTSRGVGTMVVP 301


Lambda     K      H
   0.319    0.139    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 364
Number of extensions: 12
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 317
Length of database: 309
Length adjustment: 27
Effective length of query: 290
Effective length of database: 282
Effective search space:    81780
Effective search space used:    81780
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

Align candidate WP_005457887.1 SACCYDRAFT_RS16645 (acetylglutamate kinase)
to HMM TIGR00761 (argB: acetylglutamate kinase (EC 2.7.2.8))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00761.hmm
# target sequence database:        /tmp/gapView.5313.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00761  [M=231]
Accession:   TIGR00761
Description: argB: acetylglutamate kinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    2.6e-84  268.3   0.3    3.2e-84  268.1   0.3    1.1  1  lcl|NCBI__GCF_000244975.1:WP_005457887.1  SACCYDRAFT_RS16645 acetylglutama


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000244975.1:WP_005457887.1  SACCYDRAFT_RS16645 acetylglutamate kinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  268.1   0.3   3.2e-84   3.2e-84       1     231 []      38     276 ..      38     276 .. 0.97

  Alignments for each domain:
  == domain 1  score: 268.1 bits;  conditional E-value: 3.2e-84
                                 TIGR00761   1 tiViKiGGaais..elleelakdiaklrkegiklvivHGGgpeinelleklgievefvnglRvTdketl 67 
                                               t+V+K+GG+a+   el++++a+d+++lr  g+++v+vHGGgp+i+++l++lgi+ ef++glRvT+ et+
  lcl|NCBI__GCF_000244975.1:WP_005457887.1  38 TVVVKYGGNAMVddELKRAFAQDMVFLRIAGLRPVVVHGGGPQITAMLDRLGIKGEFKGGLRVTTPETM 106
                                               69*********9999****************************************************** PP

                                 TIGR00761  68 evvemvligkvnkelvallekhgikavGltgkDgqlltaekldke......dlgyvGeikkvnkellea 130
                                               +vv+mvl+g+v +elv l++ hg +avG++g+D++l+tae+ +        d+g+vGe+  vn++++ +
  lcl|NCBI__GCF_000244975.1:WP_005457887.1 107 DVVRMVLVGQVSRELVGLINAHGPYAVGISGEDARLFTAERKQATvdgepvDVGLVGEVVDVNPDAVLD 175
                                               ****************************************555555588******************** PP

                                 TIGR00761 131 llkagiipviaslaldeegqllNvnaDtaAaelAaaleAekLvlLtdvaGileg..dkksliselelee 197
                                               +++ag ipv++++a d +g ++NvnaDtaA++lA+al AekLv+Ltdv+G++++  d++sl+++++++e
  lcl|NCBI__GCF_000244975.1:WP_005457887.1 176 IVNAGRIPVVSTVAPDTDGVVHNVNADTAAGALASALRAEKLVVLTDVEGLYANwpDRSSLVDRIDADE 244
                                               ********************************************************************* PP

                                 TIGR00761 198 ieqlikqavikgGmipKveaalealesgvkkvvi 231
                                               +e+l+ +  +++GmipK+ea+l+a+++gv+++++
  lcl|NCBI__GCF_000244975.1:WP_005457887.1 245 LENLLPS--LASGMIPKMEACLRAIRGGVRRAHV 276
                                               ******9..7*********************996 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (231 nodes)
Target sequences:                          1  (309 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 7.83
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis