Align 3-isopropylmalate dehydratase large subunit; EC 4.2.1.33 (characterized)
to candidate WP_006748151.1 THITH_RS10455 3-isopropylmalate dehydratase large subunit
Query= CharProtDB::CH_024771 (466 letters) >NCBI__GCF_000227685.2:WP_006748151.1 Length = 469 Score = 587 bits (1512), Expect = e-172 Identities = 291/460 (63%), Positives = 351/460 (76%), Gaps = 1/460 (0%) Query: 3 KTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGLRAHGRPVRQPGKTFATMD 62 +TLY+KL+ AHVV+E E+ T LLYIDRHLVHEVTSPQAF+GLR GR + A D Sbjct: 4 QTLYDKLWQAHVVHEEEDGTALLYIDRHLVHEVTSPQAFEGLRLAGRKPWRSDSVLAVPD 63 Query: 63 HNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNHPYQGIVHVMGPEQGVTLPGM 122 HNV T+ ++ ++R Q++ L +N EFG++ + + QGIVHV+GPEQG TLPGM Sbjct: 64 HNVPTEHREAGIADPVSRTQVETLERNVGEFGLKFFPMRDLRQGIVHVIGPEQGATLPGM 123 Query: 123 TIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVQGKAAPGITAKD 182 T+VCGDSHT+THGA GALAFGIGTSEVEHVLATQ L Q ++++M + V G+ PG+TAKD Sbjct: 124 TVVCGDSHTSTHGALGALAFGIGTSEVEHVLATQCLWQKKSRSMLVRVDGELRPGVTAKD 183 Query: 183 IVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVAPDETTFNY 242 +VLAIIG G+AGGTG+ +EF G AIR LS+EGRMT+CNMAIE GA+AG+VA DE T Y Sbjct: 184 VVLAIIGTIGTAGGTGYAIEFGGAAIRSLSVEGRMTVCNMAIEAGARAGMVAVDEKTIEY 243 Query: 243 VKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEISPQVTWGTNPGQVISVND 302 V+GR AP G+ F+ A W+ L +D A FD VV L A I PQV+WGT+P V+ V+ Sbjct: 244 VRGRPQAPAGELFERAALSWRELVSDPDARFDRVVVLDAATIEPQVSWGTSPEMVVPVSG 303 Query: 303 NIPDPASFADPVERASAEKALAYMGLKPGIPLTEVAIDKVFIGSCTNSRIEDLRAAAEIA 362 +PDPA DPV KAL YMGL+PG P+ + DKVFIGSCTNSRIEDLR AA + Sbjct: 304 RVPDPALEKDPVRAEGMRKALRYMGLEPGTPIDGIRPDKVFIGSCTNSRIEDLREAAAVV 363 Query: 363 KGRKVAPGVQ-ALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLNPG 421 +GR+ A VQ A+VVPGSG VK QAEAEGLD++F++AGFEWR PGCSMCLAMN DRL PG Sbjct: 364 RGRRKADSVQLAMVVPGSGLVKQQAEAEGLDRVFLDAGFEWREPGCSMCLAMNADRLEPG 423 Query: 422 ERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFAD 461 ERCASTSNRNFEGRQG+GGRTHLVSPA+AAAAAV GHF D Sbjct: 424 ERCASTSNRNFEGRQGQGGRTHLVSPALAAAAAVAGHFVD 463 Lambda K H 0.317 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 787 Number of extensions: 27 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 466 Length of database: 469 Length adjustment: 33 Effective length of query: 433 Effective length of database: 436 Effective search space: 188788 Effective search space used: 188788 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate WP_006748151.1 THITH_RS10455 (3-isopropylmalate dehydratase large subunit)
to HMM TIGR00170 (leuC: 3-isopropylmalate dehydratase, large subunit (EC 4.2.1.33))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00170.hmm # target sequence database: /tmp/gapView.9371.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00170 [M=466] Accession: TIGR00170 Description: leuC: 3-isopropylmalate dehydratase, large subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 7e-235 765.8 0.0 8.4e-235 765.6 0.0 1.0 1 lcl|NCBI__GCF_000227685.2:WP_006748151.1 THITH_RS10455 3-isopropylmalate Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000227685.2:WP_006748151.1 THITH_RS10455 3-isopropylmalate dehydratase large subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 765.6 0.0 8.4e-235 8.4e-235 3 462 .. 4 463 .. 2 467 .. 1.00 Alignments for each domain: == domain 1 score: 765.6 bits; conditional E-value: 8.4e-235 TIGR00170 3 ktlyeklfdahvvkeaenetdllyidrhlvhevtspqafeglraagrkvrrvdktlatldhnistesrd 71 +tly+kl++ahvv+e+e++t llyidrhlvhevtspqafeglr agrk r d+ la dhn++te r+ lcl|NCBI__GCF_000227685.2:WP_006748151.1 4 QTLYDKLWQAHVVHEEEDGTALLYIDRHLVHEVTSPQAFEGLRLAGRKPWRSDSVLAVPDHNVPTEHRE 72 7******************************************************************** PP TIGR00170 72 veikeekaklqvkeleknvkefgvklfdlssaeqgivhvvgpeegltlpgktivcgdshtathgafgal 140 i + +++ qv++le+nv efg+k+f +++ +qgivhv+gpe+g tlpg+t+vcgdsht+thga+gal lcl|NCBI__GCF_000227685.2:WP_006748151.1 73 AGIADPVSRTQVETLERNVGEFGLKFFPMRDLRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGALGAL 141 ********************************************************************* PP TIGR00170 141 afgigtsevehvlatqtlkqaraktlkievegklakgitakdiilaiigkigvaggtgyvvefageair 209 afgigtsevehvlatq l+q++++++ ++v+g+l +g+takd++laiig ig+aggtgy +ef g air lcl|NCBI__GCF_000227685.2:WP_006748151.1 142 AFGIGTSEVEHVLATQCLWQKKSRSMLVRVDGELRPGVTAKDVVLAIIGTIGTAGGTGYAIEFGGAAIR 210 ********************************************************************* PP TIGR00170 210 dlsmeermtvcnmaieagakagliapdettfeyvkdrkyapkgkefekavaywktlktdegakfdkvvt 278 +ls+e+rmtvcnmaieaga+ag++a de t+eyv++r++ap g+ fe a w+ l +d++a+fd+vv+ lcl|NCBI__GCF_000227685.2:WP_006748151.1 211 SLSVEGRMTVCNMAIEAGARAGMVAVDEKTIEYVRGRPQAPAGELFERAALSWRELVSDPDARFDRVVV 279 ********************************************************************* PP TIGR00170 279 leakdispqvtwgtnpgqvlsvneevpdpksladpvekasaekalaylglepgtklkdikvdkvfigsc 347 l+a i pqv+wgt+p++v++v++ vpdp+ dpv+ +++ kal+y+glepgt+++ i+ dkvfigsc lcl|NCBI__GCF_000227685.2:WP_006748151.1 280 LDAATIEPQVSWGTSPEMVVPVSGRVPDPALEKDPVRAEGMRKALRYMGLEPGTPIDGIRPDKVFIGSC 348 ********************************************************************* PP TIGR00170 348 tnsriedlraaaevvkgkkvadnvklalvvpgsglvkkqaekegldkifleagfewreagcslclgmnn 416 tnsriedlr+aa+vv+g+++ad+v+la+vvpgsglvk+qae+egld++fl+agfewre+gcs+cl+mn lcl|NCBI__GCF_000227685.2:WP_006748151.1 349 TNSRIEDLREAAAVVRGRRKADSVQLAMVVPGSGLVKQQAEAEGLDRVFLDAGFEWREPGCSMCLAMNA 417 ********************************************************************* PP TIGR00170 417 dvldeyercastsnrnfegrqgkgarthlvspamaaaaavagkfvd 462 d+l+++ercastsnrnfegrqg+g+rthlvspa+aaaaavag+fvd lcl|NCBI__GCF_000227685.2:WP_006748151.1 418 DRLEPGERCASTSNRNFEGRQGQGGRTHLVSPALAAAAAVAGHFVD 463 *********************************************9 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (466 nodes) Target sequences: 1 (469 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 9.25 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory