Protein WP_007473232.1 in Caminibacter mediatlanticus TB-2
Annotation: NCBI__GCF_000170735.1:WP_007473232.1
Length: 420 amino acids
Source: GCF_000170735.1 in NCBI
Candidate for 11 steps in Amino acid biosynthesis
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-phenylalanine biosynthesis | PPYAT | lo | Beta-phenylalanine transaminase; Aromatic beta-amino acid aminotransferase; Beta-phenylalanine aminotransferase; VpAT; EC 2.6.1.- (characterized) | 31% | 91% | 208 | Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 | 51% | 458.0 |
| L-tyrosine biosynthesis | tyrB | lo | Beta-phenylalanine transaminase; Aromatic beta-amino acid aminotransferase; Beta-phenylalanine aminotransferase; VpAT; EC 2.6.1.- (characterized) | 31% | 91% | 208 | Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 | 51% | 458.0 |
| L-arginine biosynthesis | argD | lo | Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 (uncharacterized) | 32% | 88% | 158.3 | Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 | 51% | 458.0 |
| L-proline biosynthesis | argD | lo | Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 (uncharacterized) | 32% | 88% | 158.3 | Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 | 51% | 458.0 |
| L-arginine biosynthesis | lysJ | lo | [LysW]-aminoadipate semialdehyde/glutamate semialdehyde transaminase; EC 2.6.1.118; EC 2.6.1.124 (uncharacterized) | 34% | 80% | 153.3 | Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 | 51% | 458.0 |
| L-lysine biosynthesis | lysJ | lo | [LysW]-aminoadipate semialdehyde/glutamate semialdehyde transaminase; EC 2.6.1.118; EC 2.6.1.124 (uncharacterized) | 34% | 80% | 153.3 | Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 | 51% | 458.0 |
| L-proline biosynthesis | lysJ | lo | [LysW]-aminoadipate semialdehyde/glutamate semialdehyde transaminase; EC 2.6.1.118; EC 2.6.1.124 (uncharacterized) | 34% | 80% | 153.3 | Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 | 51% | 458.0 |
| L-arginine biosynthesis | argD'B | lo | succinylornithine transaminase (EC 2.6.1.81) (characterized) | 30% | 85% | 144.4 | Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 | 51% | 458.0 |
| L-lysine biosynthesis | lysN | lo | 2-aminoadipate transaminase; 2-aminoadipate aminotransferase; L-2AA aminotransferase; EC 2.6.1.39 (characterized) | 30% | 74% | 137.9 | Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 | 51% | 458.0 |
| L-proline biosynthesis | OAT | lo | ornithine aminotransferase (EC 2.6.1.13) (characterized) | 30% | 67% | 134.4 | Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 | 51% | 458.0 |
| L-lysine biosynthesis | dapC | lo | Acetylornithine/succinyldiaminopimelate aminotransferase; ACOAT; DapATase; Succinyldiaminopimelate transferase; EC 2.6.1.11; EC 2.6.1.17 (uncharacterized) | 30% | 77% | 129.4 | Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 | 51% | 458.0 |
Sequence Analysis Tools
View WP_007473232.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MGIFNEAQKYIVGGVNSPVRAFKSVGGEPPFIEKGEGAYIFDIEGNKYLDYIQSWGPLIF
GHCDKDTQNAIIEAVKKGVSFGAPTKVEVELAKEVLELFPHLNLIRFVNSGTEATMSAIR
LARGYTGKDDIIKFEGCYHGHSDSLLVSAGSGAATFGVPSSPGVPADFTKHTLLAKYNDI
ESVKKCFENGDVGCVIIEPIAGNMSLVPGEKEFLGELREICNHYGAVLIFDEVMSGFRAS
LRGSFDIYGIKADIVTFGKVIGGGMPVGAFAGKKEIMEKLSPVGPVYQAGTLSGNPVAMS
AGLTVIKKLKENPEIYKELEDKAKKLMEGFSEISKENNIDFNYNVIGSMFGFFFNKKLPK
NFDEVNESDTKRYAKFHNNMLKSGFYFAPSAYETGFICTPMNEKDIENTITTYSKIVKEI
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory