Align threonine ammonia-lyase (EC 4.3.1.19) (characterized)
to candidate WP_010959715.1 MCA_RS01745 threonine ammonia-lyase, biosynthetic
Query= BRENDA::P04968 (514 letters) >NCBI__GCF_000008325.1:WP_010959715.1 Length = 509 Score = 540 bits (1390), Expect = e-158 Identities = 275/502 (54%), Positives = 362/502 (72%), Gaps = 2/502 (0%) Query: 15 EYLRAVLRAPVYEAAQVTPLQKMEKLSSRLDNVILVKREDRQPVHSFKLRGAYAMMAGLT 74 +Y+ +LRA VY+ AQ TPL LS RLDN +L+KRED QPV SFKLRGAY +A LT Sbjct: 4 KYIEKILRARVYDVAQETPLDPAPGLSRRLDNTVLIKREDLQPVFSFKLRGAYNKIASLT 63 Query: 75 EEQKAHGVITASAGNHAQGVAFSSARLGVKALIVMPTATADIKVDAVRGFGGEVLLHGAN 134 E +A GVI ASAGNHAQGVA ++ RLG++A+IVMP T IKVDAVR GGEV+LHG Sbjct: 64 PEARAAGVIAASAGNHAQGVALAAQRLGIRAVIVMPCTTPHIKVDAVRNRGGEVVLHGDA 123 Query: 135 FDEAKAKAIELSQQQGFTWVPPFDHPMVIAGQGTLALELLQQDAH-LDRVFVPVGGGGLA 193 +DEA A+EL++ Q T+V P+D P VIAGQGT+ +E+L+Q + +FVPVGGGGL Sbjct: 124 YDEAYEHALELARDQCLTFVHPYDDPEVIAGQGTIGMEILRQHQDAIHAIFVPVGGGGLI 183 Query: 194 AGVAVLIKQLMPQIKVIAVEAEDSACLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFR 253 AG+A +K + P I+VI VE DS CL AL A V L +VGLFA+GVAVK++G E F Sbjct: 184 AGIAAYVKFVRPDIRVIGVEPVDSDCLHRALKAKRRVILKQVGLFADGVAVKQVGKEPFH 243 Query: 254 LCQEYLDDIITVDSDAICAAMKDLFEDVRAVAEPSGALALAGMKKYIALHNIRGERLAHI 313 L +++D+++TVD+D ICAA+KD+F+D R++AEP+GAL +AG+KKY+A I+ L I Sbjct: 244 LAHQWVDEVVTVDTDEICAAIKDIFDDTRSIAEPAGALGIAGLKKYVAETGIKNACLVAI 303 Query: 314 LSGANVNFHGLRYVSERCELGEQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFAD 373 SGAN+NF LR+V+ER E+GE+RE LLAVTIPE GSFL+FC++LG R++TEFNYRF D Sbjct: 304 ESGANINFDRLRHVAERAEIGEKRELLLAVTIPERPGSFLEFCRVLGRRNITEFNYRFFD 363 Query: 374 AKNACIFVGVRLSRGLEERKEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQE 433 K A +FVG+ ++ G +R+ +++ G+ V+DL+ +E+A H+RYMVGG L E Sbjct: 364 EKAAQVFVGLPVASGAIDRESLVREFERQGFGVLDLTGNELAIEHIRYMVGGHAPKLLDE 423 Query: 434 RLYSFEFPESPGALLRFLNTLGTYWNISLFHYRSHGTDYGRVLAAFELGDHE-PDFETRL 492 ++YSFEFPE PGALLRFL+ +G WNISLFHYR+HG +GRVL ++ E F L Sbjct: 424 QVYSFEFPERPGALLRFLSIMGGRWNISLFHYRNHGAAFGRVLMGIQVPKPERKAFREFL 483 Query: 493 NELGYDCHDETNNPAFRFFLAG 514 +GY +ET NPA+R F G Sbjct: 484 EAIGYAFKEETQNPAYRLFAGG 505 Lambda K H 0.321 0.138 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 693 Number of extensions: 32 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 514 Length of database: 509 Length adjustment: 35 Effective length of query: 479 Effective length of database: 474 Effective search space: 227046 Effective search space used: 227046 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
Align candidate WP_010959715.1 MCA_RS01745 (threonine ammonia-lyase, biosynthetic)
to HMM TIGR01124 (ilvA: threonine ammonia-lyase, biosynthetic (EC 4.3.1.19))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01124.hmm # target sequence database: /tmp/gapView.7746.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01124 [M=499] Accession: TIGR01124 Description: ilvA_2Cterm: threonine ammonia-lyase, biosynthetic Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.4e-252 824.6 0.1 1.6e-252 824.4 0.1 1.0 1 lcl|NCBI__GCF_000008325.1:WP_010959715.1 MCA_RS01745 threonine ammonia-ly Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000008325.1:WP_010959715.1 MCA_RS01745 threonine ammonia-lyase, biosynthetic # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 824.4 0.1 1.6e-252 1.6e-252 2 498 .. 5 502 .. 4 503 .. 0.99 Alignments for each domain: == domain 1 score: 824.4 bits; conditional E-value: 1.6e-252 TIGR01124 2 ylrailkarvyeaavetplekaaklserlknrvllkredlqpvfsfklrGaynkmaqlsaeqkakGvia 70 y+++il+arvy++a+etpl++a ls+rl+n+vl+kredlqpvfsfklrGaynk+a+l++e++a+Gvia lcl|NCBI__GCF_000008325.1:WP_010959715.1 5 YIEKILRARVYDVAQETPLDPAPGLSRRLDNTVLIKREDLQPVFSFKLRGAYNKIASLTPEARAAGVIA 73 99******************************************************************* PP TIGR01124 71 asaGnhaqGvalsakklGvkavivmpettpeikvdavkafGgevvlhGenydeakakalelaqekgltf 139 asaGnhaqGval+a++lG++avivmp+ttp+ikvdav+++GgevvlhG++ydea+++alela+++ ltf lcl|NCBI__GCF_000008325.1:WP_010959715.1 74 ASAGNHAQGVALAAQRLGIRAVIVMPCTTPHIKVDAVRNRGGEVVLHGDAYDEAYEHALELARDQCLTF 142 ********************************************************************* PP TIGR01124 140 iapfddplviaGqGtvalellrqveedldavfvpvGGGGliaGvaalvkqllpeikvigveaedsaalk 208 ++p+ddp viaGqGt+++e+lrq+++ ++a+fvpvGGGGliaG+aa+vk ++p+i+vigve+ ds++l+ lcl|NCBI__GCF_000008325.1:WP_010959715.1 143 VHPYDDPEVIAGQGTIGMEILRQHQDAIHAIFVPVGGGGLIAGIAAYVKFVRPDIRVIGVEPVDSDCLH 211 ********************************************************************* PP TIGR01124 209 qaleaGervkldqvGlfadGvavkevGdetfrlckeylddivlvdtdevcaaikdvfedtravlepaGa 277 +al+a +rv l+qvGlfadGvavk+vG+e f+l+++++d++v+vdtde+caaikd+f+dtr+++epaGa lcl|NCBI__GCF_000008325.1:WP_010959715.1 212 RALKAKRRVILKQVGLFADGVAVKQVGKEPFHLAHQWVDEVVTVDTDEICAAIKDIFDDTRSIAEPAGA 280 ********************************************************************* PP TIGR01124 278 lalaGlkkyvakkgiedktlvailsGanlnfdrlryvseraelGeqreallavtipeekGsllkfvevl 346 l++aGlkkyva+ gi++ lvai sGan+nfdrlr+v+erae+Ge+re llavtipe++Gs+l+f++vl lcl|NCBI__GCF_000008325.1:WP_010959715.1 281 LGIAGLKKYVAETGIKNACLVAIESGANINFDRLRHVAERAEIGEKRELLLAVTIPERPGSFLEFCRVL 349 ********************************************************************* PP TIGR01124 347 GeraitefnyrladdekahifvGvqlaee.eerkellarleeagykvvdltddelaklhvrylvGGraa 414 G r+itefnyr+ d+++a++fvG+ +a+ +r++l++++e++g+ v+dlt +ela h+ry+vGG a lcl|NCBI__GCF_000008325.1:WP_010959715.1 350 GRRNITEFNYRFFDEKAAQVFVGLPVASGaIDRESLVREFERQGFGVLDLTGNELAIEHIRYMVGGHAP 418 ************************9988748************************************** PP TIGR01124 415 kvenerlysfefperpGallkfletlqaewnislfhyrnhGadyGrvlvglevpdeeaeefeqflaelg 483 k+ +e++ysfefperpGall+fl+ + ++wnislfhyrnhGa++Grvl+g++vp+ e ++f +fl+ +g lcl|NCBI__GCF_000008325.1:WP_010959715.1 419 KLLDEQVYSFEFPERPGALLRFLSIMGGRWNISLFHYRNHGAAFGRVLMGIQVPKPERKAFREFLEAIG 487 ********************************************************************* PP TIGR01124 484 yryedetenpayrlf 498 y +++et+npayrlf lcl|NCBI__GCF_000008325.1:WP_010959715.1 488 YAFKEETQNPAYRLF 502 **************9 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (499 nodes) Target sequences: 1 (509 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 8.73 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory