Alignments for a candidate for asd-S-transferase in Methanosarcina barkeri Fusaro

GapMind for Amino acid biosynthesis

Alignments for a candidate for asd-S-transferase in Methanosarcina barkeri Fusaro

Align L-aspartate semialdehyde sulfurtransferase (EC 2.8.1.16) (characterized)
to candidate WP_011307062.1 MBAR_RS11065 CBS domain-containing protein

Query= BRENDA::Q8TPT4
         (500 letters)



>NCBI__GCF_000195895.1:WP_011307062.1
          Length = 500

 Score =  891 bits (2303), Expect = 0.0
 Identities = 443/500 (88%), Positives = 475/500 (95%)

Query: 1   MVEKSVHEINKKIEDGSVNVVTAEEMVGIVENLGVEGAAREVDVVTTGTFGAMCSSGLML 60
           MV+KSVHEIN+KIEDGSVNVVTAEEMV IVE LG EGAA+EVDVVTTGTFGAMCSSGLML
Sbjct: 1   MVKKSVHEINRKIEDGSVNVVTAEEMVTIVEELGAEGAAKEVDVVTTGTFGAMCSSGLML 60

Query: 61  NLGHSEPPIKIQKLWFNNVEAYSGLAAVDAYLGAAQISDTRGIQYGGAHVIEDLLRGKEL 120
           NLGHSEPPIKIQK+WFNNVEAYSGLAAVDAYLGAAQISDTRGIQYGGAHVIEDLLRGKE+
Sbjct: 61  NLGHSEPPIKIQKIWFNNVEAYSGLAAVDAYLGAAQISDTRGIQYGGAHVIEDLLRGKEI 120

Query: 121 DVHATSYGTDCYPRKVLDTRITLDDLNEAVLLNPRNAYQKYAAATNSSKRILNTYMGELL 180
           DVHATSYGTDCYPRKVLDT I+L+DLNEA+LLNPRNAYQKYAAATNSSKR L+TYMGELL
Sbjct: 121 DVHATSYGTDCYPRKVLDTIISLEDLNEAILLNPRNAYQKYAAATNSSKRTLHTYMGELL 180

Query: 181 PNFGNVTYSGAGVLSPLSNDPDYETIGMGTRIFMGGAQGYIIGNGTQHSPSSSFGTLMLK 240
           PN GNVTYSGAGVLSPLSNDP+YETIGMGTRIFMGG QGY+IGNGTQHSPSS FGTLMLK
Sbjct: 181 PNLGNVTYSGAGVLSPLSNDPNYETIGMGTRIFMGGTQGYVIGNGTQHSPSSGFGTLMLK 240

Query: 241 GNLKEMSSDYLRAASFAGYGTTLYMGIGIPIPILNEKIAASTAVRDEDIFTDILDYAVGS 300
           GNLKEM+ DYLRAASF GYG TLYMG+GIPIPILNEK+AA+TAVRDEDI T I DYAVGS
Sbjct: 241 GNLKEMNPDYLRAASFTGYGATLYMGVGIPIPILNEKLAAATAVRDEDIVTTIEDYAVGS 300

Query: 301 RDKPVIKQVNYAELRSGSIELEGKNTPTSSLSSFKNARKIANELKEWVKHGKFFVSMPVE 360
           RDKPVI++V+YAELRSGS++++GK+ PTSSLSSFKNARKIANELK WVKHGKFFVSMPVE
Sbjct: 301 RDKPVIREVSYAELRSGSVQIDGKDVPTSSLSSFKNARKIANELKAWVKHGKFFVSMPVE 360

Query: 361 KLSREGSAKSMKQTQAVPLVKDVMADFIVTIKKNQTVQDAAKKIWENSFNHLAVVSDTGE 420
           +L  EGSAK MK+TQ VPLVKDVM+ F+VTIK++QTVQDAAKKIW NSFNHL V+SD+GE
Sbjct: 361 RLCAEGSAKPMKETQVVPLVKDVMSSFVVTIKRDQTVQDAAKKIWANSFNHLTVISDSGE 420

Query: 421 LVGILTAWDISKAVAENIFDSVESVMTKKVLTCAPNEPVDLAARRLDRYGVSAMPVIDTQ 480
           LVGILTAWDISKAVAEN FDSVESVMTKKVLTCAPNEPVDLAARRLDRYGVSAMPVID Q
Sbjct: 421 LVGILTAWDISKAVAENCFDSVESVMTKKVLTCAPNEPVDLAARRLDRYGVSAMPVIDAQ 480

Query: 481 RKVLGIITSDNISKLLARRY 500
           R+VLGIITSDNISKLLARRY
Sbjct: 481 RQVLGIITSDNISKLLARRY 500


Lambda     K      H
   0.315    0.132    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 951
Number of extensions: 18
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 500
Length of database: 500
Length adjustment: 34
Effective length of query: 466
Effective length of database: 466
Effective search space:   217156
Effective search space used:   217156
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis