Alignments for a candidate for gatA in Trichormus variabilis ATCC 29413

GapMind for Amino acid biosynthesis

Alignments for a candidate for gatA in Trichormus variabilis ATCC 29413

Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (characterized)
to candidate WP_011320446.1 AVA_RS18990 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA

Query= SwissProt::O06491
         (485 letters)



>NCBI__GCF_000204075.1:WP_011320446.1
          Length = 486

 Score =  560 bits (1444), Expect = e-164
 Identities = 279/482 (57%), Positives = 367/482 (76%), Gaps = 4/482 (0%)

Query: 8   ITELKQLIHKKEIKISDLVDESYKRIQAVDDKVQAFLALDEERARAYAKELDEAVDGRSE 67
           I EL + + KKE    ++  E+   IQ ++ K+ +FL +  ++A   A+ +D  +    E
Sbjct: 4   IRELHEQLVKKERSAVEITQETLDHIQELEPKLHSFLHITAQQALEQARAVDAKIAAGEE 63

Query: 68  HGLLFGMPIGVKDNIVTKGLRTTCSSKILENFDPIYDATVVQRLQDAEAVTIGKLNMDEF 127
            GLL G+PIGVKDN+ TKG+ TTC+S+ILENF P Y++TV Q+L DA AV +GK N+DEF
Sbjct: 64  IGLLAGIPIGVKDNMCTKGIPTTCASRILENFVPPYESTVTQKLLDAGAVVVGKTNLDEF 123

Query: 128 AMGSSTENSAYKLTKNPWNLDTVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIRQPASFCG 187
           AMGSSTENSAY++T NPW+L  VPGGSSGGSAAAVAA E   +LGSDTGGSIRQPASFCG
Sbjct: 124 AMGSSTENSAYQVTANPWDLSRVPGGSSGGSAAAVAAEECVVALGSDTGGSIRQPASFCG 183

Query: 188 VVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAFLLQAISGVDKMDSTSANVDVPD 247
           VVGLKPTYG VSRYGLVA+ASSLDQIGP  ++VED A LL+AI+G D  DSTS  V++PD
Sbjct: 184 VVGLKPTYGLVSRYGLVAYASSLDQIGPFGKSVEDTAILLKAIAGYDPKDSTSLKVEIPD 243

Query: 248 FLSSLTGDIKG---LKIAVPKEYLGEGVGKEARESVLAALKVLEGLGATWEEVSLPHSKY 304
           +++SL  D+K    L+I V KE  GEG+     ++V  A++ L+ LGA    +S P+ +Y
Sbjct: 244 YVASLKPDLKARGKLRIGVIKETFGEGLDSVVEQAVTKAIEQLQRLGAEIHVISCPNFRY 303

Query: 305 ALATYYLLSSSEASANLARFDGIRYGYRTDNADNLIDLYKQTRAEGFGNEVKRRIMLGTF 364
            + +YY+++ SEASANLAR+DG++YG+R    DNL+ +YK+TRA GFG EVKRRIM+GT+
Sbjct: 304 GVPSYYIIAPSEASANLARYDGVKYGWRAPEGDNLLSMYKRTRATGFGAEVKRRIMIGTY 363

Query: 365 ALSSGYYDAYYKKAQKVRTLIKKDFEDVFEKYDVIVGPTTPTPAFKIGENTKDPLTMYAN 424
           ALS+GYYDAYY KAQKVRTLIK+DFE+ F+  DV+V PT PT AFK GE T DP++MY N
Sbjct: 364 ALSAGYYDAYYLKAQKVRTLIKQDFENAFKNVDVLVSPTAPTTAFKAGEKTADPISMYLN 423

Query: 425 DILTIPVNLAGVPGISVPCGLAD-GLPLGLQIIGKHFDESTVYRVAHAFEQATDHHKAKP 483
           D++TIPVNLAG+PG+S+PCG  + GLP+GLQ+IGK   E  + +VA+A+EQ+T  H ++P
Sbjct: 424 DLMTIPVNLAGLPGLSLPCGFDEQGLPIGLQLIGKVLREDQLLQVAYAYEQSTSWHLSQP 483

Query: 484 EL 485
           ++
Sbjct: 484 KI 485


Lambda     K      H
   0.315    0.134    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 656
Number of extensions: 23
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 485
Length of database: 486
Length adjustment: 34
Effective length of query: 451
Effective length of database: 452
Effective search space:   203852
Effective search space used:   203852
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

Align candidate WP_011320446.1 AVA_RS18990 (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA)
to HMM TIGR00132 (gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (EC 6.3.5.-))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00132.hmm
# target sequence database:        /tmp/gapView.10671.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00132  [M=466]
Accession:   TIGR00132
Description: gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   2.7e-208  678.3   0.0     3e-208  678.2   0.0    1.0  1  lcl|NCBI__GCF_000204075.1:WP_011320446.1  AVA_RS18990 Asp-tRNA(Asn)/Glu-tR


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000204075.1:WP_011320446.1  AVA_RS18990 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  678.2   0.0    3e-208    3e-208       2     464 ..       8     476 ..       7     478 .. 0.98

  Alignments for each domain:
  == domain 1  score: 678.2 bits;  conditional E-value: 3e-208
                                 TIGR00132   2 kellkkkevsikevleeilerieavkdkinaflevtkekalkkakkldkkva..ke.kklagipiavKd 67 
                                               +e+l+kke s++e+++e+l++i++ + k+++fl++t+++al++a+++d+k+a  +e  +lagipi+vKd
  lcl|NCBI__GCF_000204075.1:WP_011320446.1   8 HEQLVKKERSAVEITQETLDHIQELEPKLHSFLHITAQQALEQARAVDAKIAagEEiGLLAGIPIGVKD 76 
                                               78999**********************************************9976656*********** PP

                                 TIGR00132  68 niavkdiettcaSkiLenyvspydatVverlkeagaliiGktNlDEFamGsstetSafgvtknPeneer 136
                                               n+++k+i+ttcaS+iLen+v+py++tV+++l +aga+++GktNlDEFamGsste+Sa++vt+nP++ +r
  lcl|NCBI__GCF_000204075.1:WP_011320446.1  77 NMCTKGIPTTCASRILENFVPPYESTVTQKLLDAGAVVVGKTNLDEFAMGSSTENSAYQVTANPWDLSR 145
                                               ********************************************************************* PP

                                 TIGR00132 137 vpGGSsgGsaaavaadlvplalgsDTGgSiRqPAsfcgvvGlKPtYGlvSRyGlvayasSldqiGilak 205
                                               vpGGSsgGsaaavaa+++++algsDTGgSiRqPAsfcgvvGlKPtYGlvSRyGlvayasSldqiG++ k
  lcl|NCBI__GCF_000204075.1:WP_011320446.1 146 VPGGSSGGSAAAVAAEECVVALGSDTGGSIRQPASFCGVVGLKPTYGLVSRYGLVAYASSLDQIGPFGK 214
                                               ********************************************************************* PP

                                 TIGR00132 206 kvedialvldvisgkDkkDstslevkveelleelkkdlk...glkvgvvkelseesldkevkekfekll 271
                                               +ved+a++l++i+g+D kDstsl+v++++++ +lk dlk   +l++gv+ke ++e+ld+ v++++ k++
  lcl|NCBI__GCF_000204075.1:WP_011320446.1 215 SVEDTAILLKAIAGYDPKDSTSLKVEIPDYVASLKPDLKargKLRIGVIKETFGEGLDSVVEQAVTKAI 283
                                               ***********************************955533379************************* PP

                                 TIGR00132 272 ekleelgaeivevslpsvklalaiYyiispsEassnlarydgiryGkrveelkslkelyaktRsegfge 340
                                               e+l++lgaei  +s+p++++ ++ Yyii+psEas+nlarydg++yG r+ e ++l  +y++tR+ gfg+
  lcl|NCBI__GCF_000204075.1:WP_011320446.1 284 EQLQRLGAEIHVISCPNFRYGVPSYYIIAPSEASANLARYDGVKYGWRAPEGDNLLSMYKRTRATGFGA 352
                                               ********************************************************************* PP

                                 TIGR00132 341 evkrRimlGayalskeyydkyykkAqkvrtliidefeklfeevDvivsptaptlafklgekaedplemy 409
                                               evkrRim+G+yals++yyd+yy+kAqkvrtli+++fe++f++vDv+vsptapt+afk gek++dp++my
  lcl|NCBI__GCF_000204075.1:WP_011320446.1 353 EVKRRIMIGTYALSAGYYDAYYLKAQKVRTLIKQDFENAFKNVDVLVSPTAPTTAFKAGEKTADPISMY 421
                                               ********************************************************************* PP

                                 TIGR00132 410 lsDvltvpanlaGlpaisvPlgkkekglpiGlqiigkafddkkllsvakaleqal 464
                                               l+D++t+p+nlaGlp++s+P+g +e+glpiGlq+igk++ +++ll+va+a+eq +
  lcl|NCBI__GCF_000204075.1:WP_011320446.1 422 LNDLMTIPVNLAGLPGLSLPCGFDEQGLPIGLQLIGKVLREDQLLQVAYAYEQST 476
                                               ****************************************************986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (466 nodes)
Target sequences:                          1  (486 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.02
# Mc/sec: 9.79
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis