Align Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial; Glu-AdT subunit B; EC 6.3.5.- (characterized)
to candidate WP_011320645.1 AVA_RS20035 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB
Query= SwissProt::Q9FV81 (550 letters) >NCBI__GCF_000204075.1:WP_011320645.1 Length = 491 Score = 525 bits (1351), Expect = e-153 Identities = 271/486 (55%), Positives = 358/486 (73%), Gaps = 12/486 (2%) Query: 69 DYEAVIGIETHVQLSTLTKAFCSCSNNYGSYPNTSICPVCMGLPGALPVLNSKVVEFGVR 128 +YEA+IG+ETH QLST TK F S S +G+ PNT+I PVCMGLPG LPVLN KV+E+ V+ Sbjct: 10 EYEAIIGLETHCQLSTNTKIFSSSSTAFGADPNTNIDPVCMGLPGVLPVLNEKVLEYAVK 69 Query: 129 LGLALNCDLSLKSKFDRKQYFYPDLPKGYQISQFDIPIASGGYVDVD-IPLEFGGGHRRF 187 GLALNC ++ SKFDRKQYFYPDLPK YQISQ+D+PIA G+++++ + E +R Sbjct: 70 AGLALNCQIAKYSKFDRKQYFYPDLPKNYQISQYDLPIAEHGWLEIELLDAEGNPKRKRI 129 Query: 188 GITRVHMEEDAGKLLHSDT-----GDYSQVDLNRAGVPLLEIVSEPDMRSGIEAAEYACE 242 GITR+HMEEDAGKL+H+ + YS VD NRAGVPL+EIVSEPD+R+G EAAEYA E Sbjct: 130 GITRLHMEEDAGKLVHAGSDRISGSTYSLVDYNRAGVPLVEIVSEPDIRTGQEAAEYAQE 189 Query: 243 MQRIARYLGVSNGNMQEGSLRCDVNISIRPIGQAEFGTKVEIKNLNAFSAISRAIDFEIS 302 ++R+ RYLGVS+GNMQEGSLRCDVNIS+RP+GQ +FGTKVEIKN+N+FSAI +AI+ EI Sbjct: 190 LRRVMRYLGVSDGNMQEGSLRCDVNISVRPVGQEKFGTKVEIKNMNSFSAIQKAIEHEIE 249 Query: 303 RQALLYNQGKADQIVTETRLWEEGAQKTVTMRKKEGLADYRYFPEPDLPEVILTQEYVDS 362 RQ G ++I+ ETRLWEEG+Q+T++MR KEG +DYRYFPEPDL + +++ + Sbjct: 250 RQIEAIESG--EKIIQETRLWEEGSQRTISMRTKEGSSDYRYFPEPDLAPIEVSEAQLSQ 307 Query: 363 IRASLPELPEAKRRRYEA-MGLGMQDVLFLANDVSVAEYFDAVIGKGAEVKLAANWIMSD 421 R LPELP KR RYE+ +GL D L DV+V++YF+A I GA K AANWI D Sbjct: 308 WRGELPELPAQKRHRYESELGLSAYDTRVLTEDVTVSQYFEAAIASGANPKAAANWITQD 367 Query: 422 IAAYLKNEKLSINDIKLTPQELAELIAAIKDGTISGKIGKEILFELLAKGGTVKGMIKAK 481 IAAYL +KLSI +I LTP LA++I I+ G IS K+ L ELL G + + + Sbjct: 368 IAAYLNKQKLSIAEIGLTPANLADVITRIETGKISNAQAKQKLPELLT-GLSPEKAFAGQ 426 Query: 482 DLVQITDPAEIEKMVIQVVSENPKQLEQYRSGKTKLQGYFAGQVMKMSKGKANPGLLNKI 541 +L I+D + +E +V +V++ NPK+LE+YR+G L+G+F GQV+K + +A+P L N++ Sbjct: 427 EL--ISDLSVLEPIVDEVIAANPKELEKYRNGNINLKGFFVGQVLKKTNKRADPKLTNEL 484 Query: 542 LLEKLN 547 + +KLN Sbjct: 485 VEKKLN 490 Lambda K H 0.317 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 760 Number of extensions: 36 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 550 Length of database: 491 Length adjustment: 35 Effective length of query: 515 Effective length of database: 456 Effective search space: 234840 Effective search space used: 234840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 52 (24.6 bits)
Align candidate WP_011320645.1 AVA_RS20035 (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB)
to HMM TIGR00133 (gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit (EC 6.3.5.-))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00133.hmm # target sequence database: /tmp/gapView.12045.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00133 [M=481] Accession: TIGR00133 Description: gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 7e-187 607.6 0.0 7.9e-187 607.4 0.0 1.0 1 lcl|NCBI__GCF_000204075.1:WP_011320645.1 AVA_RS20035 Asp-tRNA(Asn)/Glu-tR Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000204075.1:WP_011320645.1 AVA_RS20035 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 607.4 0.0 7.9e-187 7.9e-187 2 481 .] 9 490 .. 8 490 .. 0.97 Alignments for each domain: == domain 1 score: 607.4 bits; conditional E-value: 7.9e-187 TIGR00133 2 ieyelviGlEvHvqlntksKlFckcsneaqeakpNtnvcpvclglPGalPvlNkeavkkAlklalalns 70 +eye++iGlE+H+ql t++K+F+++s+ + pNtn++pvc+glPG+lPvlN+++++ A+k +laln+ lcl|NCBI__GCF_000204075.1:WP_011320645.1 9 TEYEAIIGLETHCQLSTNTKIFSSSSTAFGA-DPNTNIDPVCMGLPGVLPVLNEKVLEYAVKAGLALNC 76 79***************************99.9************************************ PP TIGR00133 71 kivsevsvFdRKhYfYpDlPkgyqitqqdlPiaedGkleieleeke.....keigierlhlEeDtgksq 134 +i +++s+FdRK+YfYpDlPk+yqi+q+dlPiae+G leiel + e k+igi+rlh+EeD+gk++ lcl|NCBI__GCF_000204075.1:WP_011320645.1 77 QI-AKYSKFDRKQYFYPDLPKNYQISQYDLPIAEHGWLEIELLDAEgnpkrKRIGITRLHMEEDAGKLV 144 *5.57************************************9988888999****************** PP TIGR00133 135 ykesdk...dkqslvDfNRsgvPLlEiVtkPdlksakearaflkklrqilryleisdgdleeGsmRvDv 200 +++sd+ +++slvD+NR+gvPL+EiV++Pd+++++ea+++ ++lr+++ryl++sdg+++eGs+R+Dv lcl|NCBI__GCF_000204075.1:WP_011320645.1 145 HAGSDRisgSTYSLVDYNRAGVPLVEIVSEPDIRTGQEAAEYAQELRRVMRYLGVSDGNMQEGSLRCDV 213 *****99999*********************************************************** PP TIGR00133 201 NvsirlkGqekegtrvEiKNlnslksiekaieyEieRqkkllkkgeevkqetrafdekksitvslRkKe 269 N+s+r++Gqek+gt+vEiKN+ns+++i+kaie+EieRq++++++ge++ qetr ++e +++t+s+R Ke lcl|NCBI__GCF_000204075.1:WP_011320645.1 214 NISVRPVGQEKFGTKVEIKNMNSFSAIQKAIEHEIERQIEAIESGEKIIQETRLWEEGSQRTISMRTKE 282 ********************************************************************* PP TIGR00133 270 eseDYRYfpePdlppieideevvkekveeklpelPeakrirlkkeyglseedakvlvsdlelldafeev 338 s+DYRYfpePdl pie++e ++++ ++++lpelP +kr+r+++e+gls++d++vl++d+++ ++fe + lcl|NCBI__GCF_000204075.1:WP_011320645.1 283 GSSDYRYFPEPDLAPIEVSEAQLSQ-WRGELPELPAQKRHRYESELGLSAYDTRVLTEDVTVSQYFEAA 350 **********************999.******************************************* PP TIGR00133 339 vklikepklavnWileellgeLnkkkislaeallkpeelaeliklikegkisqksakelleellenkkd 407 + ++pk+a+nWi+++++++Lnk+k+s+ae l+p +la +i i+ gkis+ +ak+ l ell+ + lcl|NCBI__GCF_000204075.1:WP_011320645.1 351 IASGANPKAAANWITQDIAAYLNKQKLSIAEIGLTPANLADVITRIETGKISNAQAKQKLPELLTGLS- 418 **************************************************************998655. PP TIGR00133 408 pkklieklgliqisdekelvkiveevikenpkevekyksgkekalkflvGqvmkktkgradpkevekll 476 p+k + + isd + l iv+evi+ npke+eky++g ++ +f+vGqv+kkt+ radpk +++l+ lcl|NCBI__GCF_000204075.1:WP_011320645.1 419 PEKAFAGQ--ELISDLSVLEPIVDEVIAANPKELEKYRNGNINLKGFFVGQVLKKTNKRADPKLTNELV 485 55544444..5589******************************************************* PP TIGR00133 477 kells 481 +++l+ lcl|NCBI__GCF_000204075.1:WP_011320645.1 486 EKKLN 490 **996 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (481 nodes) Target sequences: 1 (491 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 9.63 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory