Alignments for a candidate for aspS2 in Rhodobacter sphaeroides ATCC 17029

GapMind for Amino acid biosynthesis

Alignments for a candidate for aspS2 in Rhodobacter sphaeroides ATCC 17029

Align aspartyl-tRNA(Asp/Asn) synthetase (EC 6.1.1.23) (characterized)
to candidate WP_011338512.1 RSPH17029_RS12475 aspartate--tRNA(Asp/Asn) ligase

Query= reanno::Dino:3609247
         (590 letters)



>NCBI__GCF_000015985.1:WP_011338512.1
          Length = 591

 Score =  993 bits (2567), Expect = 0.0
 Identities = 476/591 (80%), Positives = 523/591 (88%), Gaps = 1/591 (0%)

Query: 1   MHAFRTHTCAELTKDAVGQTVRLSGWVHRVRDHGGVLFIDLRDHYGMTQVLCDPDSPVFS 60
           MHA+R+HTC EL    VGQ VRLSGWVHRVRDHGGVLF+DLRDHYG+TQV+ D DSP F+
Sbjct: 1   MHAYRSHTCTELNAAHVGQEVRLSGWVHRVRDHGGVLFLDLRDHYGITQVIADADSPAFA 60

Query: 61  QVEKVRSEWCIRIDGTVKARDESLINPKIPTGEIEVFIRDMEVLGTAEELPLPVFGDQEY 120
           ++E VR+EW IRI+G VKARD SL+NPK+ TGEIEV+   M VLG A+ELPLPVFG+ +Y
Sbjct: 61  ELETVRAEWVIRIEGRVKARDASLVNPKLATGEIEVYATGMSVLGAADELPLPVFGETDY 120

Query: 121 PEETRLKYRFLDLRRESLHDNIMLRSKVVQSIRKRMWDIDFTEFQTPIITASSPEGARDF 180
           PEETRL YRFLDLRRE LH N+MLRS VV+S+R RMW   F EFQTPIITASSPEGARDF
Sbjct: 121 PEETRLTYRFLDLRREKLHQNMMLRSNVVRSLRNRMWGAGFNEFQTPIITASSPEGARDF 180

Query: 181 LVPSRLHPGKFYALPQAPQQFKQLIMVGGFDKYFQIAPCFRDEDPRADRSPTDFYQLDLE 240
           LVPSRLHPGKFYALPQAPQQFKQLIMV GFD+YFQIAPCFRDEDPRADRSPTDFYQLD+E
Sbjct: 181 LVPSRLHPGKFYALPQAPQQFKQLIMVAGFDRYFQIAPCFRDEDPRADRSPTDFYQLDIE 240

Query: 241 MSFVSQQDVFDTIQPVIAGIFEEFGGGRRVDTDWPLISYRDSALWYGTDKPDLRNPIKMQ 300
           MSFV Q+DVF  +QPVI G+FEEFG G+RVD DWP I+YRD+ LWYG+DKPDLRNPIKMQ
Sbjct: 241 MSFVEQEDVFAAVQPVIQGLFEEFGHGKRVDADWPRIAYRDAMLWYGSDKPDLRNPIKMQ 300

Query: 301 IVSDHFAGSGFAIFAKLLEQEGTEIRAIPAPGGGSRKFCDRMNKFAQEQGLPGMGYIFWR 360
           +VS+HFAGSGFAIFAKLLE EGTEIRAIPAP GGSRKFCDRMN FAQ QGLPGMGYIFWR
Sbjct: 301 VVSEHFAGSGFAIFAKLLENEGTEIRAIPAPTGGSRKFCDRMNAFAQSQGLPGMGYIFWR 360

Query: 361 DQGQG-MEAAGPLAKNIGPERTEAIRQQLGLQVGDAAFFLGGKPASFEAVAGRARTVIGE 419
               G MEAAGPLAKNIGPERTEAIRQQLGL  GDAAFFLGGKP +FEAVAGRART IG 
Sbjct: 361 KGDDGAMEAAGPLAKNIGPERTEAIRQQLGLGEGDAAFFLGGKPETFEAVAGRARTEIGR 420

Query: 420 ELGLIDQDRFAFAWIVDFPMYEKDDEGRIDFSHNPFSMPQGGMAALEGDPLEVLGYQYDL 479
           ELGL +++ F FAWIVDFPMYEKDDEG+IDFSHNPFSMPQGGM ALEGDPL+V  YQYDL
Sbjct: 421 ELGLTEENCFKFAWIVDFPMYEKDDEGKIDFSHNPFSMPQGGMEALEGDPLKVHAYQYDL 480

Query: 480 ACNGYELVSGAIRNHKLDIMYKAFEIAGYGADEVEKRFGGMVNAFKYGPPPHGGCAAGID 539
           ACNGYEL+SG IRNHK +IM+KAFE+AGY  +EVEKRFGGMV AF+YG PPHGGCAAGID
Sbjct: 481 ACNGYELISGGIRNHKPEIMFKAFELAGYPKEEVEKRFGGMVKAFRYGAPPHGGCAAGID 540

Query: 540 RIVMLLADTANIREVIMFPMNQRAEDLMMNAPSEPTGEQLRDLSLRVIPQE 590
           RIVMLLAD ANIREVIMFPMNQRAEDL+M APSEPT EQLR+L LRV+P++
Sbjct: 541 RIVMLLADEANIREVIMFPMNQRAEDLLMGAPSEPTNEQLRELRLRVVPKD 591


Lambda     K      H
   0.322    0.141    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1277
Number of extensions: 47
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 590
Length of database: 591
Length adjustment: 37
Effective length of query: 553
Effective length of database: 554
Effective search space:   306362
Effective search space used:   306362
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis