Alignments for a candidate for asnA in Frankia alni ACN14A

GapMind for Amino acid biosynthesis

Alignments for a candidate for asnA in Frankia alni ACN14A

Align asparagine synthetase B, glutamine-hydrolyzing; EC 6.3.5.4 (characterized)
to candidate WP_011606722.1 FRAAL_RS24565 asparagine synthase (glutamine-hydrolyzing)

Query= CharProtDB::CH_002444
         (554 letters)



>NCBI__GCF_000058485.1:WP_011606722.1
          Length = 613

 Score =  152 bits (383), Expect = 5e-41
 Identities = 152/544 (27%), Positives = 227/544 (41%), Gaps = 96/544 (17%)

Query: 1   MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNAILAHERLSIVDVNAGA 60
           MC I G      D  E +     ++  +R RGPD  G +   +A + H RL+++D+  G 
Sbjct: 1   MCGITGWVSFGQDDYEQKTVIEAMTATLRRRGPDAGGTWVGAHAAIGHRRLAVIDLVGGV 60

Query: 61  QPLYNQQK--THVLAVNGEIYNHQALRAEYGDR-YQFQTGSDCEVILALYQEKGPEFLDD 117
           QP+ + Q     VL  +GEIYNH  LR E   R +  +T SD EV+L  Y E G   +D 
Sbjct: 61  QPMVSDQDGAETVLTYSGEIYNHHELRGELVRRGHTLRTRSDTEVVLHAYLEWGDLLVDR 120

Query: 118 LQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVASEMKALV--PVCRT---- 171
           L+GM+AFA++D      L+ RD LG+ PL+   +  G +   SE KAL   P+ R     
Sbjct: 121 LEGMYAFAVWDERAGRLLLVRDRLGVKPLFYA-EIPGGVVFGSEPKALFCHPLVRPRVNA 179

Query: 172 --IKE-----FPAGSYLWSQDGEI---------------RSYYHRDWFDYDAVKDNVTDK 209
             ++E     F  G  +W+   E+               R+Y+  D     AV       
Sbjct: 180 DGLREAYSLLFNTGPTVWTGVRELAPGALATFGPHGLTERTYWALDAAAMAAVSPQAA-V 238

Query: 210 NELRQALEDSVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLH 269
            ++R  LE +  + L +DVP   LLSGGLDS++++A+      RR     R  ++     
Sbjct: 239 EQVRVHLERATAAQLEADVPLCSLLSGGLDSTVLTALLADELRRREGPHARIRSFAVDYS 298

Query: 270 SFAVGLPG-----SPDLKAAQEVANHLGTVHHEIHFTVQEGLD-AIRDVIYHIETYDVTT 323
             AV   G       D   A E + ++GT H  +       LD   R  +       +  
Sbjct: 299 DQAVQFTGDVLRTGHDAPYAVEASRYIGTDHSTVVLDPHTLLDPEHRLAVVAARDSPIGV 358

Query: 324 IRASTPMYLMSRKIKAMGIKMVLSGEGSDEVFGGYLYFH-----KAP------------- 365
               T +Y++  KI A    + LSGE +DEVFGGY +FH      AP             
Sbjct: 359 GDMDTSLYVLFGKI-AQSSTVALSGEAADEVFGGYPWFHSPAALSAPTFPWLLVTGDEAA 417

Query: 366 -----------NAKELHEETVRKLLAL--HMYD-----------------------CARA 389
                        +E   +T R  LA   H+                           R 
Sbjct: 418 MPLHPDLAATLRIEEFRADTYRDALAAVPHLDGEDPTEHRQREMAHLSLTRWLRQLLHRK 477

Query: 390 NKAMSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQ 449
           ++   A G+E RVP+ D + ++ A  + P      +G+ EK +LR       P SV  R+
Sbjct: 478 DRLSMAQGIEVRVPYCDHRLVEYAFGV-PWAVRSFDGR-EKSLLRAAGAGRCPESVLIRE 535

Query: 450 KEQF 453
           K  +
Sbjct: 536 KNHY 539


Lambda     K      H
   0.319    0.135    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 791
Number of extensions: 38
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 554
Length of database: 613
Length adjustment: 36
Effective length of query: 518
Effective length of database: 577
Effective search space:   298886
Effective search space used:   298886
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis