Alignments for a candidate for tpiA in Trichodesmium erythraeum IMS101

GapMind for Amino acid biosynthesis

Alignments for a candidate for tpiA in Trichodesmium erythraeum IMS101

Align triose-phosphate isomerase (EC 5.3.1.1) (characterized)
to candidate WP_011611066.1 TERY_RS06405 triose-phosphate isomerase

Query= BRENDA::P00943
         (253 letters)



>NCBI__GCF_000014265.1:WP_011611066.1
          Length = 241

 Score =  238 bits (606), Expect = 1e-67
 Identities = 125/248 (50%), Positives = 170/248 (68%), Gaps = 11/248 (4%)

Query: 1   MRKPIIAGNWKMHKTLAEAVQFVEDVKGHVPPADEVISVV-CAPFLFLDRLVQAADGTDL 59
           MRK IIAGNWKM+KT  EA +F+E +   +    E   VV C PF  LD + +   G+ +
Sbjct: 1   MRKTIIAGNWKMYKTQLEAKEFLEGLMSQLTKTPEEREVVLCTPFTALDFMSKILHGSLI 60

Query: 60  KIGAQTMHFADQGAYTGEVSPVMLKDLGVTYVILGHSERRQMFAETDETVNKKVLAAFTR 119
           ++GAQ +H+ D+GAYTGE+S +MLKD+GV+YVI+GHSERRQ F ETDETVN ++ AA   
Sbjct: 61  RLGAQNVHWEDEGAYTGEISGLMLKDVGVSYVIVGHSERRQYFGETDETVNMRLKAAQKH 120

Query: 120 GLIPIICCGESLEEREAGQTNAVVASQVEKALAGLTPEQVKQAVIAYEPIWAIGTGKSST 179
           GL PI+C GE+ ++R++G+T + + SQ+   L  +  E +   VIAYEPIWAIGTG +  
Sbjct: 121 GLTPILCVGETKQQRDSGETESHIFSQLANDLVDVDQENL---VIAYEPIWAIGTGDTCE 177

Query: 180 PEDANSVCGHIRSVVSRLFGPEAAEAIRIQYGGSVKPDNIRDFLAQQQIDGPLVGGASLE 239
            ++AN V G IR  ++          + IQYGGSVKP+N+ D +AQ++IDG LVGGASL 
Sbjct: 178 AKEANRVIGLIRGKLNN-------SNVTIQYGGSVKPNNVDDIMAQEEIDGALVGGASLN 230

Query: 240 PASFLQLV 247
           P SF +LV
Sbjct: 231 PESFSRLV 238


Lambda     K      H
   0.318    0.134    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 194
Number of extensions: 12
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 253
Length of database: 241
Length adjustment: 24
Effective length of query: 229
Effective length of database: 217
Effective search space:    49693
Effective search space used:    49693
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 46 (22.3 bits)

Align candidate WP_011611066.1 TERY_RS06405 (triose-phosphate isomerase)
to HMM TIGR00419 (tpiA: triose-phosphate isomerase (EC 5.3.1.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00419.hmm
# target sequence database:        /tmp/gapView.30357.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00419  [M=228]
Accession:   TIGR00419
Description: tim: triose-phosphate isomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    2.2e-54  170.7   0.0    2.6e-54  170.5   0.0    1.0  1  lcl|NCBI__GCF_000014265.1:WP_011611066.1  TERY_RS06405 triose-phosphate is


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000014265.1:WP_011611066.1  TERY_RS06405 triose-phosphate isomerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  170.5   0.0   2.6e-54   2.6e-54       1     228 []       5     232 ..       5     232 .. 0.93

  Alignments for each domain:
  == domain 1  score: 170.5 bits;  conditional E-value: 2.6e-54
                                 TIGR00419   1 lviinfKlnesvgkvelevaklaeevas.eagvevavappfvdldvvkdeve.seiqvaAqnvdavksG 67 
                                               ++ +n+K+  +  + ++ +  l  ++++ +++ ev++  pf  ld +++ ++ s i+++Aqnv+  + G
  lcl|NCBI__GCF_000014265.1:WP_011611066.1   5 IIAGNWKMYKTQLEAKEFLEGLMSQLTKtPEEREVVLCTPFTALDFMSKILHgSLIRLGAQNVHWEDEG 73 
                                               6899***9999999999*******9987256789******************999************** PP

                                 TIGR00419  68 aftGeisAemlkdlGakgvligHsErRsllkeadeliekkvarlkelglksvvCvgetleereaartin 136
                                               a+tGeis  mlkd+G+ +v++gHsErR ++ e+de +++++  +++ gl++++Cvget+++r++++t  
  lcl|NCBI__GCF_000014265.1:WP_011611066.1  74 AYTGEISGLMLKDVGVSYVIVGHSERRQYFGETDETVNMRLKAAQKHGLTPILCVGETKQQRDSGETES 142
                                               ***************************************************************999999 PP

                                 TIGR00419 137 nvattaaaaA....lepdvvAvEPveliGtGkpvskAeaevveksvrdhlkkvskevaesvrvlyGasv 201
                                                +  + a        e+ v+A+EP+++iGtG +    ea+ v + +r       k  + +v ++yG+sv
  lcl|NCBI__GCF_000014265.1:WP_011611066.1 143 HIFSQLANDLvdvdQENLVIAYEPIWAIGTGDTCEAKEANRVIGLIR------GKLNNSNVTIQYGGSV 205
                                               998877654445558999*****************************......5556889********* PP

                                 TIGR00419 202 taaedaelaaqldvdGvLlasavlkae 228
                                               + ++  + +aq ++dG+L+++a+l +e
  lcl|NCBI__GCF_000014265.1:WP_011611066.1 206 KPNNVDDIMAQEEIDGALVGGASLNPE 232
                                               ***********************9986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (228 nodes)
Target sequences:                          1  (241 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 5.69
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis