Align ATP phosphoribosyltransferase (EC 2.4.2.17) (characterized)
to candidate WP_011650624.1 RL_RS04600 ATP phosphoribosyltransferase
Query= reanno::Smeli:SMc00917 (231 letters) >NCBI__GCF_000009265.1:WP_011650624.1 Length = 231 Score = 388 bits (996), Expect = e-113 Identities = 193/231 (83%), Positives = 210/231 (90%) Query: 1 MTVTIALPSKGRMKDEASAIFERAGMKIAAVGSDRSYRGRVEGWDDVEIAYLSASEIARE 60 MT+TIALPSKGRMK++ASAIFERAGM I+AVG+DRSYRGRVEGWDDVEIA+LSASEI+RE Sbjct: 1 MTITIALPSKGRMKEDASAIFERAGMTISAVGNDRSYRGRVEGWDDVEIAFLSASEISRE 60 Query: 61 IGSGAVDFGVTGEDLVREGLADADARVDFAARLGFGHADVVVAVPEIWYDVDTMADLGDV 120 IG+G VDFGVTGEDL+REG A+ D RV+F ARLGFGHADVVVAVPEIW DVDTMADL DV Sbjct: 61 IGNGTVDFGVTGEDLMREGFAEVDKRVEFCARLGFGHADVVVAVPEIWLDVDTMADLVDV 120 Query: 121 AADFRARHGRRLAIATKYWRLTQQFFSGSHGIQLYRIVESLGATEGAPASGSADIIVDIT 180 AADFRARHGRRLAIATKYWRLTQQFFS HGIQLYRIVESLGATEGAPASGSADIIVDIT Sbjct: 121 AADFRARHGRRLAIATKYWRLTQQFFSSQHGIQLYRIVESLGATEGAPASGSADIIVDIT 180 Query: 181 STGSTLKANHLKILSDGVILRSEACLVRARKATHEGNPVIDRIVAAVRAVL 231 STGSTL+ANHLK+L DGVIL S+ACLVRARK H G P + I+ AVR+ L Sbjct: 181 STGSTLRANHLKVLQDGVILHSQACLVRARKEGHAGEPAVQAIIEAVRSAL 231 Lambda K H 0.320 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 257 Number of extensions: 3 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 231 Length of database: 231 Length adjustment: 23 Effective length of query: 208 Effective length of database: 208 Effective search space: 43264 Effective search space used: 43264 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 46 (22.3 bits)
Align candidate WP_011650624.1 RL_RS04600 (ATP phosphoribosyltransferase)
to HMM TIGR00070 (hisG: ATP phosphoribosyltransferase (EC 2.4.2.17))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00070.hmm # target sequence database: /tmp/gapView.5571.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00070 [M=183] Accession: TIGR00070 Description: hisG: ATP phosphoribosyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.5e-55 173.3 0.0 2.9e-55 173.1 0.0 1.0 1 lcl|NCBI__GCF_000009265.1:WP_011650624.1 RL_RS04600 ATP phosphoribosyltra Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000009265.1:WP_011650624.1 RL_RS04600 ATP phosphoribosyltransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 173.1 0.0 2.9e-55 2.9e-55 1 182 [. 3 207 .. 3 208 .. 0.92 Alignments for each domain: == domain 1 score: 173.1 bits; conditional E-value: 2.9e-55 TIGR00070 1 lriAlp.KGrleeetlkllekaglklskke.erkliasaed.eevevlllrakdiptyvekgaadlGit 66 ++iAlp KGr++e++ +++e+ag+++s+ +r++ ++e ++ve+ +l+a++i +++ +g +d+G+t lcl|NCBI__GCF_000009265.1:WP_011650624.1 3 ITIALPsKGRMKEDASAIFERAGMTISAVGnDRSYRGRVEGwDDVEIAFLSASEISREIGNGTVDFGVT 71 79**************************9989999999887478************************* PP TIGR00070 67 GkDlleE..sead..vvelldlgfgkcklvlAvpees.dvesledlke.........gk..riATkypn 119 G+Dl++E e d v+ ++lgfg++++v+Avpe + dv++++dl + g+ iATky + lcl|NCBI__GCF_000009265.1:WP_011650624.1 72 GEDLMREgfAEVDkrVEFCARLGFGHADVVVAVPEIWlDVDTMADLVDvaadfrarhGRrlAIATKYWR 140 *******6633335699999**************************99999***99844468******* PP TIGR00070 120 ltreylekkg.vk.veivkleGavElapllgladaIvDivetGttLrengLkiie..eilessarli 182 lt++++++++ ++ ++iv++ Ga+E ap+ g ad+IvDi++tG+tLr+n+Lk+++ +il+s+a+l+ lcl|NCBI__GCF_000009265.1:WP_011650624.1 141 LTQQFFSSQHgIQlYRIVESLGATEGAPASGSADIIVDITSTGSTLRANHLKVLQdgVILHSQACLV 207 ******987777779***************************************8667********8 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (183 nodes) Target sequences: 1 (231 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 8.13 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory