Align glutamine synthetase (EC 6.3.1.2) (characterized)
to candidate WP_011651961.1 RL_RS12340 type I glutamate--ammonia ligase
Query= BRENDA::P0A1P6 (469 letters) >NCBI__GCF_000009265.1:WP_011651961.1 Length = 469 Score = 637 bits (1644), Expect = 0.0 Identities = 308/467 (65%), Positives = 372/467 (79%), Gaps = 1/467 (0%) Query: 2 SAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFFEEGKMFDGSSIGGWKGI 61 +A +L + E++VKFVDLRFTD KGK QHVT+ V+ + F +G MFDGSSIGGWK I Sbjct: 3 TASEILKQIKENDVKFVDLRFTDPKGKLQHVTMDVVCVDEDMFADGVMFDGSSIGGWKAI 62 Query: 62 NESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYDRDPRSIAKRAEDYLRATGI 121 NESDMVLMPD T +DPFFA ST++I CDIL+P + + Y+RDPR AK+AE YL+A+GI Sbjct: 63 NESDMVLMPDTETVHMDPFFAQSTMVIVCDILDPVSGEAYNRDPRGTAKKAEAYLKASGI 122 Query: 122 ADTVLFGPEPEFFLFDDIRFGASISGSHVAIDDIEGAWNSSTKYEGGNKGHRPGVKGGYF 181 DTV GPE EFF+FDD+++ A + +D E N T YE GN GHRP VKGGYF Sbjct: 123 GDTVFVGPEAEFFVFDDVKYKADPYNTGFKLDSTELPSNDDTDYETGNLGHRPRVKGGYF 182 Query: 182 PVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAGQNEVATRFNTMTKKADEIQIYKY 241 PVPPVDSAQD+RSEM V+ +MG+VVE HHHEVA A Q+E+ +F+T+ + AD++QIYKY Sbjct: 183 PVPPVDSAQDMRSEMLTVLSEMGVVVEKHHHEVAAA-QHELGIKFDTLVRNADKMQIYKY 241 Query: 242 VVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLAKNGTNLFSGDKYAGLSEQALYYIGG 301 VVH VA+ +GKTATFMPKP+FGDNGSGMH H S+ K G F+GD+YAGLSE L+YIGG Sbjct: 242 VVHQVANAYGKTATFMPKPIFGDNGSGMHVHQSIWKGGKPTFAGDEYAGLSESCLFYIGG 301 Query: 302 VIKHAKAINALANPTTNSYKRLVPGYEAPVMLAYSARNRSASIRIPVVASPKARRIEVRF 361 +IKHAKAINA NP+TNSYKRLVPGYEAPV+LAYSARNRSAS RIP ++PKA+R+EVRF Sbjct: 302 IIKHAKAINAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPFGSNPKAKRVEVRF 361 Query: 362 PDPAANPYLCFAALLMAGLDGIKNKIHPGEAMDKNLYDLPPEEAKEIPQVAGSLEEALNA 421 PDP ANPYL FAA+LMAGLDGIKNKIHPG+AMDK+LYDLPP+E K+IP V GSL EAL + Sbjct: 362 PDPTANPYLAFAAMLMAGLDGIKNKIHPGKAMDKDLYDLPPKELKKIPTVCGSLREALES 421 Query: 422 LDLDREFLKAGGVFTDEAIDAYIALRREEDDRVRMTPHPVEFELYYS 468 LD DR+FL AGGVF D+ IDA+I L+ E R MTPHPVE+++YYS Sbjct: 422 LDKDRKFLTAGGVFDDDQIDAFIELKMAEVMRFEMTPHPVEYDMYYS 468 Lambda K H 0.318 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 785 Number of extensions: 24 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 469 Length of database: 469 Length adjustment: 33 Effective length of query: 436 Effective length of database: 436 Effective search space: 190096 Effective search space used: 190096 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate WP_011651961.1 RL_RS12340 (type I glutamate--ammonia ligase)
to HMM TIGR00653 (glnA: glutamine synthetase, type I (EC 6.3.1.2))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00653.hmm # target sequence database: /tmp/gapView.28572.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00653 [M=462] Accession: TIGR00653 Description: GlnA: glutamine synthetase, type I Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.5e-207 674.2 0.0 5.1e-207 674.0 0.0 1.0 1 lcl|NCBI__GCF_000009265.1:WP_011651961.1 RL_RS12340 type I glutamate--amm Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000009265.1:WP_011651961.1 RL_RS12340 type I glutamate--ammonia ligase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 674.0 0.0 5.1e-207 5.1e-207 2 462 .] 6 468 .. 5 468 .. 0.99 Alignments for each domain: == domain 1 score: 674.0 bits; conditional E-value: 5.1e-207 TIGR00653 2 evlkllkeenvkfvdlrfvDikGklkkveipvseleeealeegiaFDgssveGfksieesDlllkpdpe 70 e+lk +ke++vkfvdlrf+D kGkl++v++ v ++e+++ +g++FDgss+ G+k+i+esD++l+pd+e lcl|NCBI__GCF_000009265.1:WP_011651961.1 6 EILKQIKENDVKFVDLRFTDPKGKLQHVTMDVVCVDEDMFADGVMFDGSSIGGWKAINESDMVLMPDTE 74 68899**************************************************************** PP TIGR00653 71 tlvivPfraekvlrvicdvyepvtkepyerdpRsiakraeeelk.tklgdevyfGpEaEFflfdkvefk 138 t+ ++Pf a+++++++cd+ +pv +e y+rdpR +ak+ae +lk +++gd+v++GpEaEFf+fd+v+ k lcl|NCBI__GCF_000009265.1:WP_011651961.1 75 TVHMDPFFAQSTMVIVCDILDPVSGEAYNRDPRGTAKKAEAYLKaSGIGDTVFVGPEAEFFVFDDVKYK 143 ********************************************9************************ PP TIGR00653 139 easnssflevdseegewn..reveegnkgykikkkggYfdvepvDkakdirrelvlaleelglevevsH 205 ++ +++ds+e n +++e+gn g++++ kggYf+v+pvD+a+d+r+e++++l+e+g+ ve++H lcl|NCBI__GCF_000009265.1:WP_011651961.1 144 ADPYNTGFKLDSTELPSNddTDYETGNLGHRPRVKGGYFPVPPVDSAQDMRSEMLTVLSEMGVVVEKHH 212 **************988878999********************************************** PP TIGR00653 206 HEvataqaEidikfdklvkaaDeivlyKyvvknvakkhGktatFmpKplfgdngsGmHvhlslwkdgen 274 HEva aq+E++ikfd+lv++aD++++yKyvv++va+ +GktatFmpKp+fgdngsGmHvh+s+wk g++ lcl|NCBI__GCF_000009265.1:WP_011651961.1 213 HEVAAAQHELGIKFDTLVRNADKMQIYKYVVHQVANAYGKTATFMPKPIFGDNGSGMHVHQSIWKGGKP 281 ********************************************************************* PP TIGR00653 275 lfageegyagLsetalyyigGilkHakalaAltnptvnsYkRLvpGyEAPvylaysaknRsaliRiPas 343 fag+e yagLse +l+yigGi+kHaka++A+tnp++nsYkRLvpGyEAPv laysa+nRsa+ RiP lcl|NCBI__GCF_000009265.1:WP_011651961.1 282 TFAGDE-YAGLSESCLFYIGGIIKHAKAINAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPFG 349 ******.************************************************************** PP TIGR00653 344 anpkakRiEvRspDpsanpYLafaallmAgldGiknkidpgepldknlyelseeelkelGieqlpesLe 412 +npkakR+EvR+pDp+anpYLafaa+lmAgldGiknki+pg+++dk+ly+l+++elk+ i+++ sL+ lcl|NCBI__GCF_000009265.1:WP_011651961.1 350 SNPKAKRVEVRFPDPTANPYLAFAAMLMAGLDGIKNKIHPGKAMDKDLYDLPPKELKK--IPTVCGSLR 416 **********************************************************..********* PP TIGR00653 413 ealdelesdk..evlkevlgeelieafielkrkEveelrlkvhpvElekyld 462 eal++l++d+ +++++v+ ++ i+afielk++Ev ++++++hpvE +y++ lcl|NCBI__GCF_000009265.1:WP_011651961.1 417 EALESLDKDRkfLTAGGVFDDDQIDAFIELKMAEVMRFEMTPHPVEYDMYYS 468 **********99999***********************************95 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (462 nodes) Target sequences: 1 (469 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 8.20 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory