Align 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54) (characterized)
to candidate WP_011735520.1 PPRO_RS08045 2-dehydro-3-deoxyphosphooctonate aldolase
Query= BRENDA::A0A482F152 (272 letters) >NCBI__GCF_000015045.1:WP_011735520.1 Length = 272 Score = 111 bits (278), Expect = 1e-29 Identities = 84/264 (31%), Positives = 136/264 (51%), Gaps = 25/264 (9%) Query: 13 TRPIDIGSGLTVGGSS-VVMMAGPCSVESYEQTRQTAQAIAALGGRVLRGGAFKP----- 66 TR I IG G+ +G + ++AGPC +ES T + A+ + + + FK Sbjct: 2 TREIVIG-GVKIGAKRPLALVAGPCVIESELATMRQAERLMTICNALSLPLIFKASYDKA 60 Query: 67 -RTAPDSFQGLGI-EGLRILREVADEFNMLVITEALGVDNLDAVVEYADIIQIGSRNMQH 124 RT+ +++G G+ EGLRILR+V + + V+++ ++ + E D++QI + + Sbjct: 61 NRTSIGAYRGPGMREGLRILRKVKESLGLAVLSDVHSIEQVAPAAEVLDVLQIPAFLCRQ 120 Query: 125 FPLLWEVGGTSIPVLLKRGYMSTVEEWLAAAEHIASRGNEDIILCERGIR-GFDNATRNV 183 LL T + +K+G + A IAS GNE+IIL ERG G++N V Sbjct: 121 TDLLIAAAATGRVINVKKGQFLAPWDMKNVAAKIASSGNENIILTERGASFGYNNL---V 177 Query: 184 LDIGSIALVKAETSYPVIADPSHAT-----------GRVDLVVAAARAGVAAGADGLLVE 232 +D+ S +++A + YPVI D +H+ G+ + V +RA VAAG DG+ +E Sbjct: 178 VDMRSFPVMRA-SGYPVIFDATHSVQLPGGQGESSGGQREFVEFLSRAAVAAGVDGIFME 236 Query: 233 AHPTPAEALSDSTQQLPLDHLPDL 256 H P +AL D + L+ LP L Sbjct: 237 VHEEPEKALCDGPNSIALNDLPAL 260 Lambda K H 0.318 0.134 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 138 Number of extensions: 4 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 272 Length of database: 272 Length adjustment: 25 Effective length of query: 247 Effective length of database: 247 Effective search space: 61009 Effective search space used: 61009 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory