Align Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 (characterized)
to candidate WP_011765401.1 AZO_RS08455 phosphoserine phosphatase SerB
Query= SwissProt::Q12A06 (236 letters) >NCBI__GCF_000061505.1:WP_011765401.1 Length = 276 Score = 228 bits (582), Expect = 7e-65 Identities = 122/214 (57%), Positives = 153/214 (71%), Gaps = 5/214 (2%) Query: 20 KLSDFKLIAFDMDSTLINIECVDEIADAAGRKAEVAAITEAAMRGEISDYKESLRQRVAL 79 +LSDF L DMDSTLINIEC+DEIAD G KAEVAAITEAAMRGEI D++ESL +RVAL Sbjct: 65 RLSDFGLFVTDMDSTLINIECIDEIADMQGLKAEVAAITEAAMRGEI-DFRESLTRRVAL 123 Query: 80 LKGVSVASMDEVYRTRLRLNPGAARLVQACKDAGLKVLLVSGGFTFFTDRIRDELGIDYT 139 L G+ +++ VYR RL+LNPGA RL++ + AG+ +LVSGGFT+FT R++ LG D+ Sbjct: 124 LAGLPETALEAVYRERLQLNPGAERLLKGLQAAGITTVLVSGGFTYFTSRLQQHLGFDHA 183 Query: 140 RSNVLETTDGLLTGRMVDQPWGDICDGEEKRKMLLETCGQLGISPRQAIAMGDGANDLPM 199 +NVLE TDG LTGR+V GDI DG K L+ QLG++P Q IA GDGAND+PM Sbjct: 184 YANVLEVTDGRLTGRVV----GDIVDGAAKAAHLVRLRDQLGLAPAQVIAAGDGANDIPM 239 Query: 200 MGEAGLSVAYHAKPRVREQAMVAINEGGLDRLLE 233 + AG VAY AKP +R A ++ LD +L+ Sbjct: 240 LSAAGFGVAYRAKPVLRAVASCCLDHVALDGILK 273 Lambda K H 0.319 0.136 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 193 Number of extensions: 5 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 236 Length of database: 276 Length adjustment: 24 Effective length of query: 212 Effective length of database: 252 Effective search space: 53424 Effective search space used: 53424 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
Align candidate WP_011765401.1 AZO_RS08455 (phosphoserine phosphatase SerB)
to HMM TIGR00338 (serB: phosphoserine phosphatase SerB (EC 3.1.3.3))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00338.hmm # target sequence database: /tmp/gapView.3566.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00338 [M=219] Accession: TIGR00338 Description: serB: phosphoserine phosphatase SerB Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.4e-71 226.6 0.0 2e-71 226.1 0.0 1.2 1 lcl|NCBI__GCF_000061505.1:WP_011765401.1 AZO_RS08455 phosphoserine phosph Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000061505.1:WP_011765401.1 AZO_RS08455 phosphoserine phosphatase SerB # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 226.1 0.0 2e-71 2e-71 13 217 .. 68 273 .. 59 275 .. 0.97 Alignments for each domain: == domain 1 score: 226.1 bits; conditional E-value: 2e-71 TIGR00338 13 kkklvvfDlDstlieeEvIdeiaklaGveeeVseiTerAmrgeldFkeslreRvkllkglpvellkkv. 80 +l+v D+Dstli++E+Ideia + G + eV++iTe Amrge+dF+esl+ Rv+ll glp+ l+ v lcl|NCBI__GCF_000061505.1:WP_011765401.1 68 DFGLFVTDMDSTLINIECIDEIADMQGLKAEVAAITEAAMRGEIDFRESLTRRVALLAGLPETALEAVy 136 5689***********************************************************999983 PP TIGR00338 81 eeklelteGveelvkkLkekgykvaviSGgFdlvaeklkekLgldavfaNrLevedgkltGkvegeivd 149 +e+l+l +G+e l k L+ +g+ ++++SGgF++++ +l+++Lg d + aN Lev+dg+ltG+v g+ivd lcl|NCBI__GCF_000061505.1:WP_011765401.1 137 RERLQLNPGAERLLKGLQAAGITTVLVSGGFTYFTSRLQQHLGFDHAYANVLEVTDGRLTGRVVGDIVD 205 589****************************************************************** PP TIGR00338 150 esakaktllkllekegislektvavGDGanDlsmikaAglgiafnakpvlkekadiviekkdltdile 217 aka l++l + g+ +++++a GDGanD++m++aAg+g+a+ akpvl+ +a +++++ l +il+ lcl|NCBI__GCF_000061505.1:WP_011765401.1 206 GAAKAAHLVRLRDQLGLAPAQVIAAGDGANDIPMLSAAGFGVAYRAKPVLRAVASCCLDHVALDGILK 273 **************************************************************999987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (219 nodes) Target sequences: 1 (276 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00 # Mc/sec: 9.38 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory