Align 3-dehydroquinate dehydratase (EC 4.2.1.10) (characterized)
to candidate WP_011779024.1 MVAN_RS08980 type II 3-dehydroquinate dehydratase
Query= BRENDA::N1V364 (155 letters) >NCBI__GCF_000015305.1:WP_011779024.1 Length = 147 Score = 185 bits (470), Expect = 2e-52 Identities = 90/142 (63%), Positives = 108/142 (76%) Query: 14 RKLLVLNGPNLNLLGTREPQIYGSDTLEDAETLAARAAEAHGLAVECLQSNHEGVLIDAI 73 R+LL++NGPNLNLLGTR+P++YG+ TL + E A A GL V +QSNHEGVL+DAI Sbjct: 4 RRLLLVNGPNLNLLGTRQPEVYGATTLAEIEARVAEVAAEAGLQVRSVQSNHEGVLVDAI 63 Query: 74 HAARGTAAGIVINPGGYTHTSVALRDALSGVDLPVVEVHISNIHRREEFRHHSYISGIAV 133 HAAR AGI+INP Y+HTSVA+ DAL V LPV EVH+SNIH REEFRHHSY+S +A Sbjct: 64 HAARTDCAGIIINPAAYSHTSVAIADALRSVGLPVAEVHLSNIHAREEFRHHSYVSAVAD 123 Query: 134 AVIAGAGISGYKFAVDLLASRL 155 V+AGAG GY+ AV LA RL Sbjct: 124 MVVAGAGPLGYEMAVRFLADRL 145 Lambda K H 0.317 0.133 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 68 Number of extensions: 1 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 155 Length of database: 147 Length adjustment: 16 Effective length of query: 139 Effective length of database: 131 Effective search space: 18209 Effective search space used: 18209 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 43 (21.2 bits)
Align candidate WP_011779024.1 MVAN_RS08980 (type II 3-dehydroquinate dehydratase)
to HMM TIGR01088 (aroQ: 3-dehydroquinate dehydratase, type II (EC 4.2.1.10))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01088.hmm # target sequence database: /tmp/gapView.1000.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01088 [M=141] Accession: TIGR01088 Description: aroQ: 3-dehydroquinate dehydratase, type II Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.2e-60 186.6 0.3 9.2e-60 186.5 0.3 1.0 1 lcl|NCBI__GCF_000015305.1:WP_011779024.1 MVAN_RS08980 type II 3-dehydroqu Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000015305.1:WP_011779024.1 MVAN_RS08980 type II 3-dehydroquinate dehydratase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 186.5 0.3 9.2e-60 9.2e-60 2 140 .. 6 144 .. 5 145 .. 0.98 Alignments for each domain: == domain 1 score: 186.5 bits; conditional E-value: 9.2e-60 TIGR01088 2 ilvlnGPnlnlLGkrepkvyGsltleeieelleeaakelevevevfqsnsegelidkihealeqvdgiv 70 +l++nGPnlnlLG+r+p+vyG++tl eie+ + e a+e++++v +qsn+eg l+d+ih a + gi+ lcl|NCBI__GCF_000015305.1:WP_011779024.1 6 LLLVNGPNLNLLGTRQPEVYGATTLAEIEARVAEVAAEAGLQVRSVQSNHEGVLVDAIHAARTDCAGII 74 799****************************************************************** PP TIGR01088 71 inpaalthtsvalrDalaavslPvvevhlsnvhareefrkksvlaevakGvivGlGakgyklalealve 139 inpaa++htsva++Dal +v lPv evhlsn+hareefr++s++++va+ v+ G G+ gy++a+++l++ lcl|NCBI__GCF_000015305.1:WP_011779024.1 75 INPAAYSHTSVAIADALRSVGLPVAEVHLSNIHAREEFRHHSYVSAVADMVVAGAGPLGYEMAVRFLAD 143 ******************************************************************987 PP TIGR01088 140 a 140 lcl|NCBI__GCF_000015305.1:WP_011779024.1 144 R 144 5 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (141 nodes) Target sequences: 1 (147 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 5.00 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory