Alignments for a candidate for hisN in Mycolicibacterium vanbaalenii PYR-1

GapMind for Amino acid biosynthesis

Alignments for a candidate for hisN in Mycolicibacterium vanbaalenii PYR-1

Align histidinol-phosphatase (EC 3.1.3.15) (characterized)
to candidate WP_011779146.1 MVAN_RS09600 histidinol-phosphatase

Query= BRENDA::Q8NS80
         (260 letters)



>NCBI__GCF_000015305.1:WP_011779146.1
          Length = 259

 Score =  345 bits (885), Expect = e-100
 Identities = 171/260 (65%), Positives = 204/260 (78%), Gaps = 4/260 (1%)

Query: 1   MSKYADDLALALELAELADSITLDRFEASDLEVSSKPDMTPVSDADLATEEALREKIATA 60
           MS   DDL LAL LA+ AD++T+ RF A DL V +KPDMTP +DADL  E+ LR ++  +
Sbjct: 1   MSTLVDDLNLALRLADEADALTMQRFGAVDLRVETKPDMTPATDADLDAEKLLRARLTES 60

Query: 61  RPADSILGEEFGGDVEFSGRQWIIDPIDGTKNYVRGVPVWATLIALLDNGKPVAGVISAP 120
           RP DS+ GEEFGG  EF+GRQW++DPIDGTKN+VRGVPVW+TLIALL +G PV GVISAP
Sbjct: 61  RPQDSVFGEEFGGTREFTGRQWVVDPIDGTKNFVRGVPVWSTLIALLVDGVPVVGVISAP 120

Query: 121 ALARRWWASEGAGAWRTFNGSSPRKLSVSQVSKLDDASLSFSSL-SGWAERDLRDQFVSL 179
           AL RRWWA EG GA+ +F G + R++SVS V+ ++ ASLSFS L +GW E+  R +FV L
Sbjct: 121 ALGRRWWAGEGQGAFTSFGGDT-RRISVSAVADMEAASLSFSDLATGWEEK--RSRFVEL 177

Query: 180 TDTTWRLRGYGDFFSYCLVAEGAVDIAAEPEVSLWDLAPLSILVTEAGGKFTSLAGVDGP 239
            D  WR+RGYGDF+SYCLVAEGAVDIA EPEV LWDLAPL ILV EAGG+FT+LAG  GP
Sbjct: 178 LDAVWRMRGYGDFWSYCLVAEGAVDIAVEPEVKLWDLAPLDILVREAGGRFTNLAGEPGP 237

Query: 240 HGGDAVATNGILHDETLDRL 259
           HGG AVATNG+LHD  L  L
Sbjct: 238 HGGSAVATNGLLHDAVLSAL 257


Lambda     K      H
   0.315    0.133    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 281
Number of extensions: 16
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 260
Length of database: 259
Length adjustment: 24
Effective length of query: 236
Effective length of database: 235
Effective search space:    55460
Effective search space used:    55460
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 47 (22.7 bits)

Align candidate WP_011779146.1 MVAN_RS09600 (histidinol-phosphatase)
to HMM TIGR02067 (hisN: histidinol-phosphatase (EC 3.1.3.15))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR02067.hmm
# target sequence database:        /tmp/gapView.1948.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR02067  [M=252]
Accession:   TIGR02067
Description: his_9_HisN: histidinol-phosphatase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    4.1e-91  290.9   0.0    4.7e-91  290.7   0.0    1.0  1  lcl|NCBI__GCF_000015305.1:WP_011779146.1  MVAN_RS09600 histidinol-phosphat


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000015305.1:WP_011779146.1  MVAN_RS09600 histidinol-phosphatase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  290.7   0.0   4.7e-91   4.7e-91       2     251 ..       8     257 ..       7     258 .. 0.97

  Alignments for each domain:
  == domain 1  score: 290.7 bits;  conditional E-value: 4.7e-91
                                 TIGR02067   2 lalalelaeaageailkyfrasdlkvdkksdktpVteADraaEeaireliaakfPddgilGEEfgeeee 70 
                                               l+lal+la+ a++ ++++f+a dl v++k+d+tp t+AD  aE+++r+ +++++P+d+++GEEfg ++e
  lcl|NCBI__GCF_000015305.1:WP_011779146.1   8 LNLALRLADEADALTMQRFGAVDLRVETKPDMTPATDADLDAEKLLRARLTESRPQDSVFGEEFGGTRE 76 
                                               67999**************************************************************** PP

                                 TIGR02067  71 daeyvWvlDPiDGTksFirGvPvwgtLiaLlekgkpvlGvisqPalgerfvaakgegallng.ge.rel 137
                                                 +++Wv+DPiDGTk+F+rGvPvw+tLiaLl +g pv+Gvis+Palg+r++a +g+ga+++  g+ r++
  lcl|NCBI__GCF_000015305.1:WP_011779146.1  77 FTGRQWVVDPIDGTKNFVRGVPVWSTLIALLVDGVPVVGVISAPALGRRWWAGEGQGAFTSFgGDtRRI 145
                                               ***********************************************************9864447*** PP

                                 TIGR02067 138 rvsevaklsdAvlvttspkaledeeereafeklrrkarltryggdcyayalvAsGrvdlvveaelspyD 206
                                               +vs+va++++A+l++++  a   ee+r++f +l ++++++r++gd+++y+lvA+G+vd++ve+e++++D
  lcl|NCBI__GCF_000015305.1:WP_011779146.1 146 SVSAVADMEAASLSFSDL-ATGWEEKRSRFVELLDAVWRMRGYGDFWSYCLVAEGAVDIAVEPEVKLWD 213
                                               ******************.8999999******************************************* PP

                                 TIGR02067 207 iaalipiieeAggvitdwkGkeaeeggeavaaanaalhdevlell 251
                                               +a+l +++ eAgg +t+++G++  +gg+a +a+n  lhd+vl +l
  lcl|NCBI__GCF_000015305.1:WP_011779146.1 214 LAPLDILVREAGGRFTNLAGEPGPHGGSA-VATNGLLHDAVLSAL 257
                                               *****************************.99**********987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (252 nodes)
Target sequences:                          1  (259 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.01
# Mc/sec: 6.50
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis