Align threonine synthase (EC 4.2.3.1) (characterized)
to candidate WP_011781502.1 MVAN_RS21820 threonine synthase
Query= BRENDA::P9WG59 (360 letters) >NCBI__GCF_000015305.1:WP_011781502.1 Length = 359 Score = 618 bits (1593), Expect = 0.0 Identities = 305/353 (86%), Positives = 329/353 (93%) Query: 7 ATHQPWPGVIAAYRDRLPVGDDWTPVTLLEGGTPLIAATNLSKQTGCTIHLKVEGLNPTG 66 A HQPWPG+I AYRDRLP+ D WT +TL EGGTPL+ A LS+ TGCT+HLKVEGLNPTG Sbjct: 6 AVHQPWPGLIEAYRDRLPIEDSWTTITLREGGTPLLPAPRLSEYTGCTVHLKVEGLNPTG 65 Query: 67 SFKDRGMTMAVTDALAHGQRAVLCASTGNTSASAAAYAARAGITCAVLIPQGKIAMGKLA 126 SFKDRGMTMAVT+A++ GQ+AVLCASTGNTSASAAAYAARAGITCAVL+PQGKIAMGKLA Sbjct: 66 SFKDRGMTMAVTEAVSRGQQAVLCASTGNTSASAAAYAARAGITCAVLVPQGKIAMGKLA 125 Query: 127 QAVMHGAKIIQIDGNFDDCLELARKMAADFPTISLVNSVNPVRIEGQKTAAFEIVDVLGT 186 QAVMHGAKIIQIDGNFDDCLELARK+ +DFPT+SLVNSVNP RIEGQKTAAFEIVD LG Sbjct: 126 QAVMHGAKIIQIDGNFDDCLELARKLTSDFPTVSLVNSVNPFRIEGQKTAAFEIVDALGD 185 Query: 187 APDVHALPVGNAGNITAYWKGYTEYHQLGLIDKLPRMLGTQAAGAAPLVLGEPVSHPETI 246 APDVH+LPVGNAGNITAYWKGYTEYH+ GL +LPRMLGTQAAGAAPLVLGEPVS PETI Sbjct: 186 APDVHSLPVGNAGNITAYWKGYTEYHRDGLAARLPRMLGTQAAGAAPLVLGEPVSDPETI 245 Query: 247 ATAIRIGSPASWTSAVEAQQQSKGRFLAASDEEILAAYHLVARVEGVFVEPASAASIAGL 306 ATAIRIGSPASW+SAVEAQQQS GRF+AA+DEEILAAYHLVAR EGVFVEPASAASIAGL Sbjct: 246 ATAIRIGSPASWSSAVEAQQQSGGRFVAATDEEILAAYHLVARAEGVFVEPASAASIAGL 305 Query: 307 LKAIDDGWVARGSTVVCTVTGNGLKDPDTALKDMPSVSPVPVDPVAVVEKLGL 359 LK+I+DGWVA+GSTVVCTVTGNGLKDPDTALK MP+V PVPVDPVAVV KLGL Sbjct: 306 LKSIEDGWVAKGSTVVCTVTGNGLKDPDTALKGMPAVIPVPVDPVAVVAKLGL 358 Lambda K H 0.317 0.132 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 534 Number of extensions: 17 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 360 Length of database: 359 Length adjustment: 29 Effective length of query: 331 Effective length of database: 330 Effective search space: 109230 Effective search space used: 109230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 49 (23.5 bits)
Align candidate WP_011781502.1 MVAN_RS21820 (threonine synthase)
to HMM TIGR00260 (thrC: threonine synthase (EC 4.2.3.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00260.hmm # target sequence database: /tmp/gapView.13420.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00260 [M=340] Accession: TIGR00260 Description: thrC: threonine synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4e-97 311.3 0.0 4.8e-97 311.1 0.0 1.1 1 lcl|NCBI__GCF_000015305.1:WP_011781502.1 MVAN_RS21820 threonine synthase Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000015305.1:WP_011781502.1 MVAN_RS21820 threonine synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 311.1 0.0 4.8e-97 4.8e-97 1 340 [] 15 333 .. 15 333 .. 0.97 Alignments for each domain: == domain 1 score: 311.1 bits; conditional E-value: 4.8e-97 TIGR00260 1 vvryreilevt.ekdlvdlaegstelfrspklaeevgaenlyvkelfhgPtlaFKDrglqfvavlltka 68 ++ yr l++ ++l eg t+l+ +p+l+e g +++k ++++Pt++FKDrg +++++t+a lcl|NCBI__GCF_000015305.1:WP_011781502.1 15 IEAYRDRLPIEdSWTTITLREGGTPLLPAPRLSEYTGC-TVHLKVEGLNPTGSFKDRG---MTMAVTEA 79 5679999999988899*********************9.*******************...******** PP TIGR00260 69 lelgnetvlcAtsGdtgaaaaealagkanvkvvvLyPkgkispvkeklvtalaenakvlaikGdFDdaq 137 +g+++vlcA++G+t+a+aa a+a++a++ + vL+P+gki+ +kl++a++++ak+++i+G+FDd++ lcl|NCBI__GCF_000015305.1:WP_011781502.1 80 VSRGQQAVLCASTGNTSASAA-AYAARAGITCAVLVPQGKIA--MGKLAQAVMHGAKIIQIDGNFDDCL 145 *********************.********************..************************* PP TIGR00260 138 dlvkeifedkeklklnsvNsinparieaqktyafeiveqlgkespdkvvvpvp.sgnfgailkGflekk 205 +l +++ +d + ++ vNs+np+rie+qkt+afeiv++lg ++pd +pv+ +gn++a++kG+ e++ lcl|NCBI__GCF_000015305.1:WP_011781502.1 146 ELARKLTSDFP--TVSLVNSVNPFRIEGQKTAAFEIVDALG-DAPDVHSLPVGnAGNITAYWKGYTEYH 211 *******7666..566689999*******************.*************************** PP TIGR00260 206 elg....lpieklaiaaegaadivrrflksgdlepkedkeTlstAmdignpsnveralelarrslgnle 270 + g lp ++l+ +a gaa+ v p +d+eT++tA++ig p+++ a+e+ ++s+g + lcl|NCBI__GCF_000015305.1:WP_011781502.1 212 RDGlaarLP-RMLGTQAAGAAPLVLGE-------PVSDPETIATAIRIGSPASWSSAVEAQQQSGGRFV 272 *********.**********9999877.......9*********************************9 PP TIGR00260 271 dlkesvsdeeileaikklaeeegyllephtavavaalkklvekgvsatadpaKFeevvealtgnklkdp 339 + +deeil a++++a+ eg+++ep++a+++a+l+k +e+g + ++ ++vv+ +tgn+lkdp lcl|NCBI__GCF_000015305.1:WP_011781502.1 273 AA----TDEEILAAYHLVARAEGVFVEPASAASIAGLLKSIEDG---WVAKG--STVVCTVTGNGLKDP 332 99....**************************************...99899..**************9 PP TIGR00260 340 e 340 + lcl|NCBI__GCF_000015305.1:WP_011781502.1 333 D 333 7 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (340 nodes) Target sequences: 1 (359 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 11.95 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory