Align candidate WP_011799552.1 PNAP_RS00580 (methionine synthase)
to HMM PF02965 (Met_synt_B12)
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/PF02965.21.hmm # target sequence database: /tmp/gapView.9026.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: Met_synt_B12 [M=273] Accession: PF02965.21 Description: Vitamin B12 dependent methionine synthase, activation domain Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 7.9e-122 392.0 0.0 1.6e-121 391.0 0.0 1.5 1 lcl|NCBI__GCF_000015505.1:WP_011799552.1 PNAP_RS00580 methionine synthase Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000015505.1:WP_011799552.1 PNAP_RS00580 methionine synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 391.0 0.0 1.6e-121 1.6e-121 1 273 [] 630 925 .. 630 925 .. 0.98 Alignments for each domain: == domain 1 score: 391.0 bits; conditional E-value: 1.6e-121 Met_synt_B12 1 dleelveyidWtpffqaWelkgkypkiledekvgeeakklfkdAqamLkkiieekllkakavvglfpAn 69 dl+el+++idW pffq+W+l+g +p+il+de+vg ea+++++dAq+mLk++ie ++l+a++v++l+pAn lcl|NCBI__GCF_000015505.1:WP_011799552.1 630 DLAELAQFIDWGPFFQTWDLAGPFPAILTDEVVGVEATRVYEDAQKMLKRLIEGRWLTASGVMALLPAN 698 699****************************************************************** PP Met_synt_B12 70 segddievyadesrseelatlhtLrqqaeke....egkpnlclaDfvapk................... 115 s+gddie+y+de+r+e+++t+h Lrqq+ek+ +p++claDfvapk lcl|NCBI__GCF_000015505.1:WP_011799552.1 699 SVGDDIEIYTDETRTEVAMTWHGLRQQTEKTavdgVMRPSRCLADFVAPKvltpeliaartraasakgq 767 ******************************9777778999*************************8887 PP Met_synt_B12 116 .esgvkDyiGlFavtaglgieelakefeaekddYsailvkaladrLaeAfaellhekvrkelWgyakde 183 + +++DyiG+Favtaglg++++ k+f+a++ddYs+i+ k+ladrLaeAfae lh++vr++lWgya+ e lcl|NCBI__GCF_000015505.1:WP_011799552.1 768 nDLKIADYIGVFAVTAGLGADKKEKAFQADHDDYSSIMFKSLADRLAEAFAEALHQRVRRDLWGYAPAE 836 7777899************************************************************** PP Met_synt_B12 184 klsneelikekYqgiRpApGYpacpdhtekktlfelldaeekigieLteslamtPaasvsGlyfahpea 252 +l ++li+e+YqgiRpApGYpacpdh+ kk++fell+a + ig+ LteslamtPaasvsG+y++hp++ lcl|NCBI__GCF_000015505.1:WP_011799552.1 837 SLGHDALIAEQYQGIRPAPGYPACPDHSVKKEMFELLHAGD-IGMALTESLAMTPAASVSGFYLSHPQS 904 *****************************************.*************************** PP Met_synt_B12 253 ryFavgkiekdqvedyakrkg 273 +yF+vgki++dq++d a+r+g lcl|NCBI__GCF_000015505.1:WP_011799552.1 905 TYFSVGKIGDDQLQDLARRRG 925 *******************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (273 nodes) Target sequences: 1 (939 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 21.90 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory