Align Homocitrate synthase; EC 2.3.3.14 (characterized)
to candidate WP_011801696.1 PNAP_RS11555 homocitrate synthase
Query= SwissProt::P05342 (385 letters) >NCBI__GCF_000015505.1:WP_011801696.1 Length = 395 Score = 381 bits (978), Expect = e-110 Identities = 199/368 (54%), Positives = 260/368 (70%), Gaps = 2/368 (0%) Query: 3 SVIIDDTTLRDGEQSAGVAFNADEKIAIARALAELGVPELEIGIPSMGEEEREVMHAIAG 62 ++II+DTTLRDGEQ+AGVAF DEK AIA ALA GVPE+EIGIP+MGEEE + ++ IA Sbjct: 4 NLIINDTTLRDGEQTAGVAFTVDEKCAIACALAAAGVPEMEIGIPAMGEEEIDCINTIAA 63 Query: 63 LGLSSRLLAWCRLCDVDLAAARSTGVTMVDLSLPVSDLMLHHKLNRDRDWALREVARLVG 122 L L + L+ W RL D DLA+A ++ LS+PVSD+ L HKL + R+W L +V R++ Sbjct: 64 LELPAALMIWGRLTDADLASALRCQADIIHLSIPVSDIHLRHKLRQSREWVLAQVTRVIE 123 Query: 123 EARMAGLEVCLGCEDASRADLEFVVQVGEVAQAAGARRLRFADTVGVMEPFGMLDRFRFL 182 +A +G ++ LG EDASRAD F+ QV AQ GA R+RFADT+GV++PF + L Sbjct: 124 KAVASGRKISLGAEDASRADAGFLSQVARRAQDCGASRIRFADTLGVLDPFTTYEAITRL 183 Query: 183 SRRLDMELEVHAHDDFGLATANTLAAVMGGATHINTTVNGLGERAGNAALEECVLALKNL 242 + +++E+HAH+D GLATANTLAA+ GATH NTTVNGLGERAGNAALEE V+ +++L Sbjct: 184 REAVHLDIEIHAHNDLGLATANTLAALRAGATHANTTVNGLGERAGNAALEEVVMGVRHL 243 Query: 243 HGIDTGIDTRGIPAISALVERASGRQVAWQKSVVGAGVFTHEAGIHVDGLLKHRRNYEGL 302 H TG+ T+ + +IS LV RASGRQVA+ KS+VG VFTHE+GIH+DGLLK+ YE Sbjct: 244 HHAQTGVSTQALVSISRLVARASGRQVAFNKSIVGEAVFTHESGIHIDGLLKNASTYESF 303 Query: 303 NPDELGRSHSLVLGKHSGAHMVRNTYRDLGIEL-ADWQSQALLGRIRAFSTRTKRRSPQP 361 +P ELGR VLGKHSG+ VR Y +G+ L D + +L RIR + R K+ + P Sbjct: 304 DPSELGRERRTVLGKHSGSQSVRQAYGAMGVVLDDDALTGRVLARIRDHAMRVKQEA-TP 362 Query: 362 AELQDFYR 369 EL+ F R Sbjct: 363 DELRGFLR 370 Lambda K H 0.320 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 475 Number of extensions: 15 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 385 Length of database: 395 Length adjustment: 31 Effective length of query: 354 Effective length of database: 364 Effective search space: 128856 Effective search space used: 128856 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
Align candidate WP_011801696.1 PNAP_RS11555 (homocitrate synthase)
to HMM TIGR02660 (nifV: homocitrate synthase (EC 2.3.3.14))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR02660.hmm # target sequence database: /tmp/gapView.3101.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02660 [M=365] Accession: TIGR02660 Description: nifV_homocitr: homocitrate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 9.2e-157 507.9 9.2 1e-156 507.7 9.2 1.0 1 lcl|NCBI__GCF_000015505.1:WP_011801696.1 PNAP_RS11555 homocitrate synthas Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000015505.1:WP_011801696.1 PNAP_RS11555 homocitrate synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 507.7 9.2 1e-156 1e-156 2 363 .. 5 367 .. 4 369 .. 0.98 Alignments for each domain: == domain 1 score: 507.7 bits; conditional E-value: 1e-156 TIGR02660 2 vlinDttLRDGEqaagvaFsaeEKlaiAkaLdeaGvdelEvGipamgeeEraairaiaalglkarllaW 70 ++inDttLRDGEq+agvaF+ +EK+aiA aL++aGv+e+E+GipamgeeE++ i++iaal+l+a+l+ W lcl|NCBI__GCF_000015505.1:WP_011801696.1 5 LIINDTTLRDGEQTAGVAFTVDEKCAIACALAAAGVPEMEIGIPAMGEEEIDCINTIAALELPAALMIW 73 79******************************************************************* PP TIGR02660 71 cRlraedieaaaevGvkavdlsvpvsdlqleaklkkdrawvleelkelvslakeeglkvsvgaeDasRa 139 Rl+++d+++a ++ + ++ls+pvsd++l++kl+++r+wvl+++++++++a +g k+s+gaeDasRa lcl|NCBI__GCF_000015505.1:WP_011801696.1 74 GRLTDADLASALRCQADIIHLSIPVSDIHLRHKLRQSREWVLAQVTRVIEKAVASGRKISLGAEDASRA 142 ********************************************************************* PP TIGR02660 140 deeflvelaevakeagakRlRfaDtvgvldPfstyelvkalraalelelElHaHnDlGlAtAntlaavk 208 d+ fl ++a+ a++ ga+R+RfaDt+gvldPf+tye++++lr+a++l++E+HaHnDlGlAtAntlaa++ lcl|NCBI__GCF_000015505.1:WP_011801696.1 143 DAGFLSQVARRAQDCGASRIRFADTLGVLDPFTTYEAITRLREAVHLDIEIHAHNDLGLATANTLAALR 211 ********************************************************************* PP TIGR02660 209 aGassvsvtvlGlGERAGnAaleevalalkellgldtgidlselkelsqlvakasgraleaqkavvGes 277 aGa+++++tv+GlGERAGnAaleev++++++l++ +tg+++++l ++s+lva+asgr+++ +k++vGe+ lcl|NCBI__GCF_000015505.1:WP_011801696.1 212 AGATHANTTVNGLGERAGNAALEEVVMGVRHLHHAQTGVSTQALVSISRLVARASGRQVAFNKSIVGEA 280 ********************************************************************* PP TIGR02660 278 vFaHEsGiHvdgllkdeatYesldPeevGrerelviGKHsgraaviealkelgleleeee.aeelleav 345 vF+HEsGiH+dgllk+++tYes+dP+e+Grer+ v+GKHsg+++v++a+ ++g+ l+++ + ++l+++ lcl|NCBI__GCF_000015505.1:WP_011801696.1 281 VFTHESGIHIDGLLKNASTYESFDPSELGRERRTVLGKHSGSQSVRQAYGAMGVVLDDDAlTGRVLARI 349 ********************************************************98761579***** PP TIGR02660 346 ravaerlKrsleeeelaa 363 r++a r+K+ ++ +el+ lcl|NCBI__GCF_000015505.1:WP_011801696.1 350 RDHAMRVKQEATPDELRG 367 **************9976 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (365 nodes) Target sequences: 1 (395 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01 # Mc/sec: 8.42 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory