Align propionate--CoA ligase (EC 6.2.1.17) (characterized)
to candidate WP_011840264.1 RSPH17029_RS01250 propionyl-CoA synthetase
Query= metacyc::MONOMER-21334 (629 letters) >NCBI__GCF_000015985.1:WP_011840264.1 Length = 630 Score = 1038 bits (2684), Expect = 0.0 Identities = 495/629 (78%), Positives = 550/629 (87%) Query: 1 MAYQDVYESWKSDPEAFWMEAAKSIDWVEAPSRALDDSNAPLYEWFTDAKVNTCWNAVDR 60 MAY +Y+ DP FWM+AA+ IDWV PS+AL D P YEWF D +VN CWNAVDR Sbjct: 1 MAYASLYKWSLEDPNGFWMKAAEQIDWVSPPSKALFDEKEPFYEWFADGQVNACWNAVDR 60 Query: 61 HVEAGRGEQTAIIYDSPITHTKRQISYVELRNRVAMLAGALRAKGVEKGDRVIIYMPMIP 120 HVEAGRG+Q AI+++SPITH+ + I+Y EL+ RVA LAGALRAKGVEKGDRVIIYMPMIP Sbjct: 61 HVEAGRGKQIAIVHESPITHSSKGITYAELQARVASLAGALRAKGVEKGDRVIIYMPMIP 120 Query: 121 EALEAMLACARLGAVHSVVFGGFAANELAVRIDDAQPKAIIAASCGLEPGRVVHYKPLLD 180 EALEAMLACARLGA+HSVVFGGFAA+ELAVRIDD PKAIIAASCGLEP RVVHYKPLLD Sbjct: 121 EALEAMLACARLGAIHSVVFGGFAAHELAVRIDDCTPKAIIAASCGLEPSRVVHYKPLLD 180 Query: 181 GAIDMAKHKPEFCVIFQREQEVAHLEEGRDYNWHGFQYGVEPAECVPVEGNHPAYILYTS 240 AI+ A HKP+FCVIFQREQEVA L EGRD WH FQYGVEPAECVPVEGNHPAYILYTS Sbjct: 181 QAIEEATHKPDFCVIFQREQEVAKLIEGRDVAWHTFQYGVEPAECVPVEGNHPAYILYTS 240 Query: 241 GTTGAPKGVLRPTAGHLVALNWTMKNIYNVDPGDVFWAASDVGWVVGHSYICYGPLIHGN 300 GTTG PKGV+R T GHLVALNWTMK IY++D GDVFWAASDVGWVVGHSYICYGPLI G Sbjct: 241 GTTGQPKGVVRATGGHLVALNWTMKAIYDIDAGDVFWAASDVGWVVGHSYICYGPLIAGA 300 Query: 301 TTIVFEGKPVGTPDAGTFWRVISEHKVKSFFTAPTAIRAVKREDPKGEMLAKYDLSHLKA 360 TTIV+EGKPVGTPDAG FWRV+ HKVK+FFTAPTA+RA+KREDP+G ++ Y+L +LKA Sbjct: 301 TTIVYEGKPVGTPDAGAFWRVMQNHKVKTFFTAPTALRAIKREDPQGALIQDYNLRNLKA 360 Query: 361 LYLAGERADPDTIIWAQKALSVPVIDHWWQTETGWTIAGNPLGIEELPTKLGSPAKAMPG 420 L+LAGERADPDTI+WAQK + VPVIDHWWQTE+GW +A NPLGIE LP K+GSP+ MPG Sbjct: 361 LFLAGERADPDTIVWAQKNVGVPVIDHWWQTESGWAMAANPLGIEALPVKIGSPSLPMPG 420 Query: 421 YDVQILDEGGHQMKPGELGAIAVKLPLPPGTLPGLWNAEARFRKSYLEHFPGYYETGDAG 480 YDV++LDEGGHQ++ G+LGAIA+KLPLPPGTLP LWNA+ RF KSYL HFPGYYETGDAG Sbjct: 421 YDVRVLDEGGHQLEAGQLGAIAIKLPLPPGTLPTLWNAQDRFVKSYLTHFPGYYETGDAG 480 Query: 481 MIDEDGYLYIMARTDDVINVAGHRLSTGGMEEVLAGHEDVAECAVIGVSDDLKGQMPLGF 540 IDEDGY+YIMARTDDVINVAGHRLSTG MEEVLA HEDVAECAVIGV+D LKGQ PLGF Sbjct: 481 YIDEDGYIYIMARTDDVINVAGHRLSTGAMEEVLASHEDVAECAVIGVTDALKGQSPLGF 540 Query: 541 LCLNNGCNRDHGDVVKEVVKLVRDKIGPVAAFKLAVVVDRLPKTRSGKILRGTMVSIADG 600 LCLN G NR DVVKE VKLVR+KIGPVA FK A VVDRLPKTRSGKILRGTMV IADG Sbjct: 541 LCLNKGSNRAAEDVVKECVKLVREKIGPVADFKRACVVDRLPKTRSGKILRGTMVKIADG 600 Query: 601 KEYKMPATIDDPAILDEIKVALQSLGYAK 629 +E+KMPATIDDPAILDEI+VAL+++GY + Sbjct: 601 QEFKMPATIDDPAILDEIRVALEAVGYPR 629 Lambda K H 0.319 0.137 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1549 Number of extensions: 44 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 629 Length of database: 630 Length adjustment: 38 Effective length of query: 591 Effective length of database: 592 Effective search space: 349872 Effective search space used: 349872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Apr 16 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory