Align 3-isopropylmalate dehydratase large subunit; EC 4.2.1.33 (characterized)
to candidate WP_011841723.1 RSPH17029_RS12730 3-isopropylmalate dehydratase large subunit
Query= CharProtDB::CH_024771 (466 letters) >NCBI__GCF_000015985.1:WP_011841723.1 Length = 475 Score = 633 bits (1632), Expect = 0.0 Identities = 309/464 (66%), Positives = 378/464 (81%), Gaps = 2/464 (0%) Query: 3 KTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGLRAHGRPVRQPGKTFATMD 62 +TLY+K++D HVV+++E+ T LLYIDRHLVHEVTSPQAF+GLR GR VR P KT A D Sbjct: 5 RTLYDKIWDDHVVHQSEDGTCLLYIDRHLVHEVTSPQAFEGLRMTGRKVRAPEKTIAVPD 64 Query: 63 HNV-STQTKDINACGEMARIQMQELIKNCKEFGVELYDLNHPYQGIVHVMGPEQGVTLPG 121 HNV +T+ +D E +RIQ++ L +N ++FG+ Y ++ QGIVH++GPEQG TLPG Sbjct: 65 HNVPTTEGRDTKIDNEESRIQVEALDRNARDFGINYYPVSDIRQGIVHIVGPEQGWTLPG 124 Query: 122 MTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVQGKAAPGITAK 181 MT+VCGDSHTATHGAFGALA GIGTSEVEHVLATQTL Q ++K MK+E+ G PG+TAK Sbjct: 125 MTVVCGDSHTATHGAFGALAHGIGTSEVEHVLATQTLIQKKSKNMKVEITGSLRPGVTAK 184 Query: 182 DIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMTLCNMAIEMGAKAGLVAPDETTFN 241 DI L++IG TG+AGGTG+V+E+CG+AIR+LSMEGRMT+CNMAIE GA+AGL+APDE TF Sbjct: 185 DITLSVIGLTGTAGGTGYVIEYCGQAIRELSMEGRMTVCNMAIEGGARAGLIAPDEKTFA 244 Query: 242 YVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTVVTLQAEEISPQVTWGTNPGQVISVN 301 YV GR HAPKG ++ A+AYWKTL TD+GA FD VVT++ E+I+P VTWGT+P V+ + Sbjct: 245 YVMGRPHAPKGAAWEAALAYWKTLFTDKGAQFDKVVTIRGEDIAPVVTWGTSPEDVLPIT 304 Query: 302 DNIPDPASFADPVERASAEKALAYMGLKPGIPLTEVAIDKVFIGSCTNSRIEDLRAAAEI 361 +P P F A A ++L YMGL PG LT++ ID VFIGSCTN RIEDLRAAAEI Sbjct: 305 ATVPAPEDFTGGKVEA-ARRSLEYMGLTPGQKLTDIKIDTVFIGSCTNGRIEDLRAAAEI 363 Query: 362 AKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLNPG 421 KG+KVAPG++A+VVPGSG V+AQAE EGL +IFI+AGFEWRL GCSMCLAMN D+L+PG Sbjct: 364 LKGKKVAPGMRAMVVPGSGLVRAQAEEEGLAQIFIDAGFEWRLAGCSMCLAMNPDQLSPG 423 Query: 422 ERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRNI 465 ERCASTSNRNFEGRQGR GRTHLVSP MAAAAA+TGH D+R++ Sbjct: 424 ERCASTSNRNFEGRQGRNGRTHLVSPGMAAAAAITGHLTDVRDL 467 Lambda K H 0.317 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 860 Number of extensions: 32 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 466 Length of database: 475 Length adjustment: 33 Effective length of query: 433 Effective length of database: 442 Effective search space: 191386 Effective search space used: 191386 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate WP_011841723.1 RSPH17029_RS12730 (3-isopropylmalate dehydratase large subunit)
to HMM TIGR00170 (leuC: 3-isopropylmalate dehydratase, large subunit (EC 4.2.1.33))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00170.hmm # target sequence database: /tmp/gapView.9837.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00170 [M=466] Accession: TIGR00170 Description: leuC: 3-isopropylmalate dehydratase, large subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5.9e-248 808.9 0.1 6.9e-248 808.7 0.1 1.0 1 lcl|NCBI__GCF_000015985.1:WP_011841723.1 RSPH17029_RS12730 3-isopropylmal Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000015985.1:WP_011841723.1 RSPH17029_RS12730 3-isopropylmalate dehydratase large subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 808.7 0.1 6.9e-248 6.9e-248 2 466 .] 4 467 .. 3 467 .. 0.99 Alignments for each domain: == domain 1 score: 808.7 bits; conditional E-value: 6.9e-248 TIGR00170 2 aktlyeklfdahvvkeaenetdllyidrhlvhevtspqafeglraagrkvrrvdktlatldhnist.es 69 ++tly+k++d hvv+++e++t llyidrhlvhevtspqafeglr +grkvr ++kt+a dhn++t e lcl|NCBI__GCF_000015985.1:WP_011841723.1 4 PRTLYDKIWDDHVVHQSEDGTCLLYIDRHLVHEVTSPQAFEGLRMTGRKVRAPEKTIAVPDHNVPTtEG 72 8***************************************************************97267 PP TIGR00170 70 rdveikeekaklqvkeleknvkefgvklfdlssaeqgivhvvgpeegltlpgktivcgdshtathgafg 138 rd++i +e++++qv++l++n+++fg++++ +s+ +qgivh+vgpe+g tlpg+t+vcgdshtathgafg lcl|NCBI__GCF_000015985.1:WP_011841723.1 73 RDTKIDNEESRIQVEALDRNARDFGINYYPVSDIRQGIVHIVGPEQGWTLPGMTVVCGDSHTATHGAFG 141 9******************************************************************** PP TIGR00170 139 alafgigtsevehvlatqtlkqaraktlkievegklakgitakdiilaiigkigvaggtgyvvefagea 207 ala gigtsevehvlatqtl+q+++k++k+e+ g l +g+takdi l +ig++g+aggtgyv+e++g+a lcl|NCBI__GCF_000015985.1:WP_011841723.1 142 ALAHGIGTSEVEHVLATQTLIQKKSKNMKVEITGSLRPGVTAKDITLSVIGLTGTAGGTGYVIEYCGQA 210 ********************************************************************* PP TIGR00170 208 irdlsmeermtvcnmaieagakagliapdettfeyvkdrkyapkgkefekavaywktlktdegakfdkv 276 ir+lsme+rmtvcnmaie ga+agliapde tf+yv +r++apkg+ +e a+aywktl td+ga+fdkv lcl|NCBI__GCF_000015985.1:WP_011841723.1 211 IRELSMEGRMTVCNMAIEGGARAGLIAPDEKTFAYVMGRPHAPKGAAWEAALAYWKTLFTDKGAQFDKV 279 ********************************************************************* PP TIGR00170 277 vtleakdispqvtwgtnpgqvlsvneevpdpksladpvekasaekalaylglepgtklkdikvdkvfig 345 vt+ ++di+p vtwgt+p++vl+++ +vp+p++++ + + a ++l+y+gl+pg+kl+dik+d vfig lcl|NCBI__GCF_000015985.1:WP_011841723.1 280 VTIRGEDIAPVVTWGTSPEDVLPITATVPAPEDFTGG-KVEAARRSLEYMGLTPGQKLTDIKIDTVFIG 347 **********************************986.456789************************* PP TIGR00170 346 sctnsriedlraaaevvkgkkvadnvklalvvpgsglvkkqaekegldkifleagfewreagcslclgm 414 sctn+riedlraaae++kgkkva++++ a+vvpgsglv++qae+egl +if++agfewr agcs+cl+m lcl|NCBI__GCF_000015985.1:WP_011841723.1 348 SCTNGRIEDLRAAAEILKGKKVAPGMR-AMVVPGSGLVRAQAEEEGLAQIFIDAGFEWRLAGCSMCLAM 415 ***************************.***************************************** PP TIGR00170 415 nndvldeyercastsnrnfegrqgkgarthlvspamaaaaavagkfvdirel 466 n+d+l+++ercastsnrnfegrqg+++rthlvsp maaaaa++g++ d+r+l lcl|NCBI__GCF_000015985.1:WP_011841723.1 416 NPDQLSPGERCASTSNRNFEGRQGRNGRTHLVSPGMAAAAAITGHLTDVRDL 467 **************************************************85 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (466 nodes) Target sequences: 1 (475 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 10.42 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory