Align 3-isopropylmalate dehydratase large subunit 1; Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1; EC 4.2.1.33 (characterized)
to candidate WP_011881753.1 3-isopropylmalate dehydratase large subunit
Query= SwissProt::P15717 (466 letters) >NCBI__GCF_000016205.1:WP_011881753.1 Length = 469 Score = 605 bits (1559), Expect = e-177 Identities = 298/468 (63%), Positives = 361/468 (77%), Gaps = 3/468 (0%) Query: 1 MAKTLYEKLFDAHVVFEAPNETPLLYIDRHLVHEVTSPQAFDGLRAHHRPVRQPGKTFAT 60 MA+TLY+KL++ HVV + T LLYIDR L+HEVTSPQAF+GL HRPV + A Sbjct: 1 MAQTLYDKLWNTHVVHTEDDGTALLYIDRQLLHEVTSPQAFEGLNVAHRPVWRISANLAV 60 Query: 61 MDHNVSTQTKDINASGEMARIQMQELIKNCNEFGVELYDLNHPYQGIVHVMGPEQGVTLP 120 DHNV T + + ++++Q+ L NC+ FG+ + +N QGIVH++GPEQG TLP Sbjct: 61 SDHNVPTTDRSHGIADPVSKLQVDTLDANCDAFGITQFKMNDLRQGIVHIIGPEQGATLP 120 Query: 121 GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVTGNAAPGITA 180 GMTIVCGDSHT+THGAFGALA GIGTSEVEHVLATQTL Q ++K M ++V G G TA Sbjct: 121 GMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQKKSKNMLVKVEGTLPRGCTA 180 Query: 181 KDIVLAIIGKTGSAGGTGHVVEFCGDAIRALSMEGRMTLCNMAIEMGAKAGLVAPDETTF 240 KDIVLAIIGK G+AGGTG+ +EF G IRAL+MEGRMT+CNMAIE GA+AG+VA D+TT Sbjct: 181 KDIVLAIIGKIGTAGGTGYAIEFGGSTIRALTMEGRMTVCNMAIEAGARAGMVAVDDTTI 240 Query: 241 NYVKGRLHAPKGRDFDEAVEYWKTLKTDDGATFDTVVALRAEEIAPQVTWGTNPGQVISV 300 +Y+KGR P G ++D+AVEYW+ K+D+GA FD VV L A EI PQVTWGT+P V S+ Sbjct: 241 DYLKGRPFVPTGAEWDQAVEYWREFKSDEGAQFDRVVELNAAEIVPQVTWGTSPEMVTSI 300 Query: 301 TDIIPDPASFSDPVERASAEKALAYMGLQPGVPLTDVAIDKVFIGSCTNSRIEDLRAAAE 360 +PDP DPV+R + E+ALAYM L+P P+ + +DK+FIGSCTN+RIED+RAAA Sbjct: 301 DARVPDPEREKDPVKREAMERALAYMALEPNTPMESINVDKIFIGSCTNARIEDIRAAAY 360 Query: 361 VAK--GRKVAPGVQ-ALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDR 417 V K R++AP V+ A+VVPGSG VKAQAE EGLDK+F +AGFEWR PGCSMCLAMN DR Sbjct: 361 VVKKLNRRIAPNVRLAMVVPGSGLVKAQAEREGLDKVFTDAGFEWREPGCSMCLAMNADR 420 Query: 418 LNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRSI 465 L PGERCASTSNRNFEGRQG GGRTHLVSPAMAAAAA+ GHF DIR + Sbjct: 421 LEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKL 468 Lambda K H 0.318 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 738 Number of extensions: 23 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 466 Length of database: 469 Length adjustment: 33 Effective length of query: 433 Effective length of database: 436 Effective search space: 188788 Effective search space used: 188788 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate WP_011881753.1 (3-isopropylmalate dehydratase large subunit)
to HMM TIGR00170 (leuC: 3-isopropylmalate dehydratase, large subunit (EC 4.2.1.33))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00170.hmm # target sequence database: /tmp/gapView.19119.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00170 [M=466] Accession: TIGR00170 Description: leuC: 3-isopropylmalate dehydratase, large subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.3e-239 781.4 0.4 1.5e-239 781.2 0.4 1.0 1 lcl|NCBI__GCF_000016205.1:WP_011881753.1 3-isopropylmalate dehydratase la Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000016205.1:WP_011881753.1 3-isopropylmalate dehydratase large subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 781.2 0.4 1.5e-239 1.5e-239 1 466 [] 1 468 [. 1 468 [. 0.99 Alignments for each domain: == domain 1 score: 781.2 bits; conditional E-value: 1.5e-239 TIGR00170 1 maktlyeklfdahvvkeaenetdllyidrhlvhevtspqafeglraagrkvrrvdktlatldhnistes 69 ma+tly+kl+++hvv+ ++++t llyidr l+hevtspqafegl++a r+v r++ la dhn++t+ lcl|NCBI__GCF_000016205.1:WP_011881753.1 1 MAQTLYDKLWNTHVVHTEDDGTALLYIDRQLLHEVTSPQAFEGLNVAHRPVWRISANLAVSDHNVPTTD 69 89******************************************************************* PP TIGR00170 70 rdveikeekaklqvkeleknvkefgvklfdlssaeqgivhvvgpeegltlpgktivcgdshtathgafg 138 r i + ++klqv +l+ n+++fg+ f +++ +qgivh++gpe+g tlpg+tivcgdsht+thgafg lcl|NCBI__GCF_000016205.1:WP_011881753.1 70 RSHGIADPVSKLQVDTLDANCDAFGITQFKMNDLRQGIVHIIGPEQGATLPGMTIVCGDSHTSTHGAFG 138 ********************************************************************* PP TIGR00170 139 alafgigtsevehvlatqtlkqaraktlkievegklakgitakdiilaiigkigvaggtgyvvefagea 207 ala gigtsevehvlatqtl q+++k++ ++veg+l +g takdi+laiigkig+aggtgy +ef g + lcl|NCBI__GCF_000016205.1:WP_011881753.1 139 ALAHGIGTSEVEHVLATQTLLQKKSKNMLVKVEGTLPRGCTAKDIVLAIIGKIGTAGGTGYAIEFGGST 207 ********************************************************************* PP TIGR00170 208 irdlsmeermtvcnmaieagakagliapdettfeyvkdrkyapkgkefekavaywktlktdegakfdkv 276 ir+l+me+rmtvcnmaieaga+ag++a d+tt++y+k+r++ p g+e+++av+yw+ +k+dega+fd+v lcl|NCBI__GCF_000016205.1:WP_011881753.1 208 IRALTMEGRMTVCNMAIEAGARAGMVAVDDTTIDYLKGRPFVPTGAEWDQAVEYWREFKSDEGAQFDRV 276 ********************************************************************* PP TIGR00170 277 vtleakdispqvtwgtnpgqvlsvneevpdpksladpvekasaekalaylglepgtklkdikvdkvfig 345 v l+a +i+pqvtwgt+p++v s++ vpdp+ dpv+++ +e+alay+ lep+t++++i+vdk+fig lcl|NCBI__GCF_000016205.1:WP_011881753.1 277 VELNAAEIVPQVTWGTSPEMVTSIDARVPDPEREKDPVKREAMERALAYMALEPNTPMESINVDKIFIG 345 ********************************************************************* PP TIGR00170 346 sctnsriedlraaaevvk..gkkvadnvklalvvpgsglvkkqaekegldkifleagfewreagcslcl 412 sctn+ried+raaa vvk +++a+nv+la+vvpgsglvk+qae+egldk+f +agfewre+gcs+cl lcl|NCBI__GCF_000016205.1:WP_011881753.1 346 SCTNARIEDIRAAAYVVKklNRRIAPNVRLAMVVPGSGLVKAQAEREGLDKVFTDAGFEWREPGCSMCL 414 *****************955599********************************************** PP TIGR00170 413 gmnndvldeyercastsnrnfegrqgkgarthlvspamaaaaavagkfvdirel 466 +mn d+l+++ercastsnrnfegrqg+g+rthlvspamaaaaa+ g+fvdir+l lcl|NCBI__GCF_000016205.1:WP_011881753.1 415 AMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKL 468 ****************************************************85 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (466 nodes) Target sequences: 1 (469 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 10.53 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory