Align L-aspartate semialdehyde sulfurtransferase iron-sulfur subunit (characterized)
to candidate WP_011881786.1 formate dehydrogenase subunit beta
Query= SwissProt::Q8TPT3 (130 letters) >NCBI__GCF_000016205.1:WP_011881786.1 Length = 304 Score = 49.3 bits (116), Expect = 5e-11 Identities = 28/78 (35%), Positives = 37/78 (47%), Gaps = 6/78 (7%) Query: 58 KALESRGAIVAILDRPIHRDEEECVECGACISVCPMNVYSFDE------TWSLCVDEKKC 111 KA S GAIV + + EE C+ CG C++ CP NV + +LC D Sbjct: 110 KACPSPGAIVQYNNGIVDFHEENCIGCGYCVTGCPFNVPRISKKDHRAYKCTLCSDRVAV 169 Query: 112 IQCGMCIKMCPHGALKLG 129 Q C+K CP GA+ G Sbjct: 170 GQEPACVKTCPTGAIVFG 187 Score = 27.3 bits (59), Expect = 2e-04 Identities = 15/57 (26%), Positives = 28/57 (49%), Gaps = 5/57 (8%) Query: 78 EEECVECG--ACISVCPMNVYSFDETWSLCVD--EKKCIQCGMCIKMCPHGALKLGE 130 ++ C+ C C+ CP + + + + VD E+ CI CG C+ CP ++ + Sbjct: 97 KDGCMHCEDPGCLKACP-SPGAIVQYNNGIVDFHEENCIGCGYCVTGCPFNVPRISK 152 Lambda K H 0.324 0.139 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 164 Number of extensions: 14 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 4 Number of HSP's successfully gapped: 2 Length of query: 130 Length of database: 304 Length adjustment: 20 Effective length of query: 110 Effective length of database: 284 Effective search space: 31240 Effective search space used: 31240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 45 (21.9 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory