Align phosphoserine transaminase (EC 2.6.1.52) (characterized)
to candidate WP_011883812.1 3-phosphoserine/phosphohydroxythreonine aminotransferase
Query= BRENDA::P23721 (362 letters) >NCBI__GCF_000016205.1:WP_011883812.1 Length = 360 Score = 403 bits (1035), Expect = e-117 Identities = 200/358 (55%), Positives = 262/358 (73%), Gaps = 1/358 (0%) Query: 3 QIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGKEFIQVAEEAEKDFRDLLN 62 ++FNFS+GPA LP EVL+QA E+ DW+G G SVME+SHRGKEF+ + E A D R+LL+ Sbjct: 2 RVFNFSAGPAALPEEVLRQAADEMLDWHGSGMSVMEMSHRGKEFMSIHETALADLRELLD 61 Query: 63 VPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYWAASAIKEAKKYCTPNVFDAK 122 VP+++++LF GGG + A VP+N+LG + +AD+V G W+ + EAKKYCTP++ + Sbjct: 62 VPASHRILFLQGGGIAENAIVPMNLLGWRKSADFVVTGSWSQKSFNEAKKYCTPHLAASG 121 Query: 123 VTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAIDETPDFGADVVVAADFSSTILSR 182 T DG EWQLSD+ AY+H C NETIDG+ E PD G DV + AD SS ILSR Sbjct: 122 KTADGFTRAPTRAEWQLSDDPAYVHLCTNETIDGVETFEIPDLG-DVPLVADVSSHILSR 180 Query: 183 PIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKANIACPSILDYSILNDNGSMFNTPP 242 P+DV++YGV++ GAQKNIG AG+T+VIVREDLL +A CPS ++ + +N S++NTPP Sbjct: 181 PMDVAKYGVLFGGAQKNIGMAGVTVVIVREDLLDRALSICPSAFEWKTVAENNSLYNTPP 240 Query: 243 TFAWYLSGLVFKWLKANGGVAEMDKINQQKAELLYGVIDNSDFYRNDVAKANRSRMNVPF 302 T+A Y++GLVF+WLK GG+A ++ N +KA+LLY ID S FY N V A RSRMNVPF Sbjct: 241 TYAIYIAGLVFQWLKRQGGLAAIEARNIEKAKLLYDTIDASGFYLNKVEPAVRSRMNVPF 300 Query: 303 QLADSALDKLFLEESFAAGLHALKGHRVVGGMRASIYNAMPLEGVKALTDFMVEFERR 360 LAD ++ FL + A GL LKGH+ VGGMRASIYNA+PLEGVKAL ++M +FERR Sbjct: 301 FLADETRNEDFLAGAKARGLLQLKGHKSVGGMRASIYNAVPLEGVKALVEYMKDFERR 358 Lambda K H 0.319 0.136 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 426 Number of extensions: 16 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 362 Length of database: 360 Length adjustment: 29 Effective length of query: 333 Effective length of database: 331 Effective search space: 110223 Effective search space used: 110223 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
Align candidate WP_011883812.1 (3-phosphoserine/phosphohydroxythreonine aminotransferase)
to HMM TIGR01364 (serC: phosphoserine transaminase (EC 2.6.1.52))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01364.hmm # target sequence database: /tmp/gapView.17662.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01364 [M=358] Accession: TIGR01364 Description: serC_1: phosphoserine transaminase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.2e-157 507.6 0.0 9.2e-157 507.4 0.0 1.0 1 lcl|NCBI__GCF_000016205.1:WP_011883812.1 3-phosphoserine/phosphohydroxyth Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000016205.1:WP_011883812.1 3-phosphoserine/phosphohydroxythreonine aminotransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 507.4 0.0 9.2e-157 9.2e-157 1 357 [. 3 358 .. 3 359 .. 0.98 Alignments for each domain: == domain 1 score: 507.4 bits; conditional E-value: 9.2e-157 TIGR01364 1 ivnFsaGPaalpeevlekaqkelldfnglglsvmeisHRskefekvveeaesdlreLlnipdnyevlfl 69 ++nFsaGPaalpeevl++a++e+ld++g+g+svme+sHR kef++++e+a dlreLl++p ++++lfl lcl|NCBI__GCF_000016205.1:WP_011883812.1 3 VFNFSAGPAALPEEVLRQAADEMLDWHGSGMSVMEMSHRGKEFMSIHETALADLRELLDVPASHRILFL 71 58******************************************************************* PP TIGR01364 70 qGGattqfaavplnllkekkvadyivtGawskkalkeakkltkevkvvase.eekkyskipdeeelelk 137 qGG+ + a vp+nll +k ad++vtG+ws+k+++eakk+++ + +as + +++ p + e++l+ lcl|NCBI__GCF_000016205.1:WP_011883812.1 72 QGGGIAENAIVPMNLLGWRKSADFVVTGSWSQKSFNEAKKYCT-PHLAASGkTADGFTRAPTRAEWQLS 139 *******************************************.555555535788************* PP TIGR01364 138 edaayvylcanetieGvefkelpevkkaplvaDlssdilsrkidvskygliyaGaqKniGpaGvtvviv 206 +d ayv+lc+neti+Gve e+p+ ++plvaD+ss+ilsr++dv+kyg++++GaqKniG+aGvtvviv lcl|NCBI__GCF_000016205.1:WP_011883812.1 140 DDPAYVHLCTNETIDGVETFEIPDLGDVPLVADVSSHILSRPMDVAKYGVLFGGAQKNIGMAGVTVVIV 208 ********************************************************************* PP TIGR01364 207 rkdllerakkelpsvldYkilaendslyntpptfaiyvlglvlkwlkekGGvkklekknqeKakllYea 275 r+dll+ra +++ps +++k++aen+slyntppt+aiy++glv++wlk++GG++++e++n eKakllY++ lcl|NCBI__GCF_000016205.1:WP_011883812.1 209 REDLLDRALSICPSAFEWKTVAENNSLYNTPPTYAIYIAGLVFQWLKRQGGLAAIEARNIEKAKLLYDT 277 ********************************************************************* PP TIGR01364 276 idesegfyknkvekkaRslmnvvFtlkkeelekeFlkeaeekglvslkGhrsvGGiRasiYnalpleev 344 id+s gfy nkve++ Rs+mnv+F l++e ++ Fl+ a+++gl++lkGh+svGG+RasiYna+ple+v lcl|NCBI__GCF_000016205.1:WP_011883812.1 278 IDAS-GFYLNKVEPAVRSRMNVPFFLADETRNEDFLAGAKARGLLQLKGHKSVGGMRASIYNAVPLEGV 345 **99.6*************************************************************** PP TIGR01364 345 qaLvdfmkeFekk 357 +aLv++mk+Fe++ lcl|NCBI__GCF_000016205.1:WP_011883812.1 346 KALVEYMKDFERR 358 **********975 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (358 nodes) Target sequences: 1 (360 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 12.11 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory