Alignments for a candidate for ilvI in Geobacter uraniireducens Rf4

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvI in Geobacter uraniireducens Rf4

Align acetohydroxyacid synthase subunit B (EC 2.2.1.6) (characterized)
to candidate WP_011940524.1 GURA_RS18945 acetolactate synthase, large subunit, biosynthetic type

Query= metacyc::MONOMER-18810
         (585 letters)



>NCBI__GCF_000016745.1:WP_011940524.1
          Length = 566

 Score =  637 bits (1644), Expect = 0.0
 Identities = 310/567 (54%), Positives = 414/567 (73%), Gaps = 4/567 (0%)

Query: 18  EMIGAEILVHALAEEGVEYVWGYPGGAVLYIYDELHKQTKFEHILVRHEQAAVHAADGYA 77
           +M GA+IL+  L  EGV+ V+GYPGG V+ +YDEL    +  HIL RHEQA VHAADGYA
Sbjct: 2   KMNGAKILLECLKLEGVDTVFGYPGGTVINLYDELFSFKEIRHILPRHEQAGVHAADGYA 61

Query: 78  RATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNVPTHAIGQDAFQECDTVGIT 137
           RATGKVGVA+ TSGPG TN +TGIATAY+DSIPMV+ITG VPT  IG DAFQE D +GIT
Sbjct: 62  RATGKVGVAIATSGPGATNTITGIATAYMDSIPMVIITGQVPTALIGNDAFQEADIIGIT 121

Query: 138 RPIVKHNFLVKDVRDLAATIKKAFFIAATGRPGPVVVDIPKDVSRNACKYEYPKSIDMRS 197
           RP  KHNFLVKDV+DLA  +KKAF+IA TGRPGPV++D+PKDV     +++YP ++++R 
Sbjct: 122 RPCTKHNFLVKDVKDLALIVKKAFYIARTGRPGPVLIDLPKDVQIATAEFKYPDTVEIRG 181

Query: 198 YNPVNKGHSGQIRKAVALLQGAERPYIYTGGGVVLANASDELRQLAALTGHPVTNTLMGL 257
           Y P  +GH  Q+ KA++LL GA+RP IY GGGV+L NA++EL  L+     PVT TLMGL
Sbjct: 182 YKPTIEGHPKQVEKAMSLLLGAKRPVIYVGGGVILGNAAEELTALSKKLEAPVTTTLMGL 241

Query: 258 GAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGNPAHFTSQARKIIHI 317
           G+FP      +G+LGMHGTY ANMA+ NCD+L+A+GARFDDRV G  A F   A KIIHI
Sbjct: 242 GSFPEDDPLSLGLLGMHGTYYANMAVSNCDLLVAVGARFDDRVTGKIASFAPHA-KIIHI 300

Query: 318 DIDPSSISKRVKVDIPIVGNVKDVLQEL--IAQIKASDIKPKREALAKWWEQIEQWRSVD 375
           D+DP+SI K V+VD+PIVG+VKDVL ++  + + +   +   ++++  W E+IE W++  
Sbjct: 301 DVDPTSIKKNVRVDLPIVGDVKDVLTQMLKVLEEQGDKLDDFKKSIVPWTEEIEGWKAKH 360

Query: 376 CLKYDRSSEIIKPQYVVEKIWELTKGDAFICSDVGQHQMWAAQFYKFDEPRRWINSGGLG 435
            + Y +S+ +IKPQ+V++K+ EL+  DA + +DVGQHQMW AQF+KF+ PR  ++SGGLG
Sbjct: 361 PMSYKQSATVIKPQFVIQKLRELSDPDAIVATDVGQHQMWTAQFFKFNRPRTLLSSGGLG 420

Query: 436 TMGVGLPYAMGIKKAFPEKEVVTITGEGSIQMCIQELSTCLQYDTPVKICSLNNGYLGMV 495
           TMG GLP AMG + AFP+++V+ I G+G  QM +QEL+T +Q    VKIC +NN +LGMV
Sbjct: 421 TMGYGLPAAMGAQAAFPDRQVIVICGDGGFQMNLQELATVVQNRLAVKICIINNNFLGMV 480

Query: 496 RQWQEIEYDNRYSHSYMDALPDFVKLAEAYGHVGMRVEKTSDVEPALREAFRLKDRTVFL 555
           RQWQE+ +D RYS + M+   DF+KLAEA+G  G +  K  +VE  +++ F      V +
Sbjct: 481 RQWQELFFDKRYSQTCMELPIDFIKLAEAFGAKGFQATKPDEVEAVIKKGFATPG-PVIM 539

Query: 556 DFQTDPTENVWPMVQAGKGISEMLLGA 582
           +F+    E V PMV AG  ++EM+L +
Sbjct: 540 EFKVAREEKVLPMVPAGASLTEMVLAS 566


Lambda     K      H
   0.319    0.135    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 898
Number of extensions: 28
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 585
Length of database: 566
Length adjustment: 36
Effective length of query: 549
Effective length of database: 530
Effective search space:   290970
Effective search space used:   290970
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

Align candidate WP_011940524.1 GURA_RS18945 (acetolactate synthase, large subunit, biosynthetic type)
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00118.hmm
# target sequence database:        /tmp/gapView.9972.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00118  [M=557]
Accession:   TIGR00118
Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   3.3e-263  860.1   0.6   3.7e-263  859.9   0.6    1.0  1  lcl|NCBI__GCF_000016745.1:WP_011940524.1  GURA_RS18945 acetolactate syntha


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000016745.1:WP_011940524.1  GURA_RS18945 acetolactate synthase, large subunit, biosynthetic type
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  859.9   0.6  3.7e-263  3.7e-263       1     555 [.       3     564 ..       3     566 .] 0.98

  Alignments for each domain:
  == domain 1  score: 859.9 bits;  conditional E-value: 3.7e-263
                                 TIGR00118   1 lkgaeilveslkkegvetvfGyPGGavlpiydaly.dselehilvrheqaaahaadGyarasGkvGvvl 68 
                                               ++ga+il+e lk egv+tvfGyPGG+v+++yd+l+  +e++hil+rheqa +haadGyara+GkvGv++
  lcl|NCBI__GCF_000016745.1:WP_011940524.1   3 MNGAKILLECLKLEGVDTVFGYPGGTVINLYDELFsFKEIRHILPRHEQAGVHAADGYARATGKVGVAI 71 
                                               78*********************************889******************************* PP

                                 TIGR00118  69 atsGPGatnlvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvtkhsflvkkaedlpei 137
                                               atsGPGatn++tgiatay+ds+P+v++tGqv+t+liG+dafqe+di+Git+p+tkh+flvk+++dl+ i
  lcl|NCBI__GCF_000016745.1:WP_011940524.1  72 ATSGPGATNTITGIATAYMDSIPMVIITGQVPTALIGNDAFQEADIIGITRPCTKHNFLVKDVKDLALI 140
                                               ********************************************************************* PP

                                 TIGR00118 138 lkeafeiastGrPGPvlvdlPkdvteaeieleveekvelpgykptvkghklqikkaleliekakkPvll 206
                                               +k+af+ia tGrPGPvl+dlPkdv+ a+ e++++++ve++gykpt++gh++q++ka+ l+  ak+Pv++
  lcl|NCBI__GCF_000016745.1:WP_011940524.1 141 VKKAFYIARTGRPGPVLIDLPKDVQIATAEFKYPDTVEIRGYKPTIEGHPKQVEKAMSLLLGAKRPVIY 209
                                               ********************************************************************* PP

                                 TIGR00118 207 vGgGviiaeaseelkelaerlkipvtttllGlGafpedhplalgmlGmhGtkeanlavseadlliavGa 275
                                               vGgGvi ++a eel+ l+++l++pvtttl+GlG+fped+pl+lg+lGmhGt++an+avs++dll+avGa
  lcl|NCBI__GCF_000016745.1:WP_011940524.1 210 VGGGVILGNAAEELTALSKKLEAPVTTTLMGLGSFPEDDPLSLGLLGMHGTYYANMAVSNCDLLVAVGA 278
                                               ********************************************************************* PP

                                 TIGR00118 276 rfddrvtgnlakfapeakiihididPaeigknvkvdipivGdakkvleellkklkee.......ekkek 337
                                               rfddrvtg++a+fap+akiihid+dP++i knv+vd+pivGd+k vl+++lk l+e+       + k++
  lcl|NCBI__GCF_000016745.1:WP_011940524.1 279 RFDDRVTGKIASFAPHAKIIHIDVDPTSIKKNVRVDLPIVGDVKDVLTQMLKVLEEQgdklddfK-KSI 346
                                               ******************************************************99998887633.444 PP

                                 TIGR00118 338 e.WlekieewkkeyilkldeeeesikPqkvikelskllkdeaivttdvGqhqmwaaqfyktkkprkfit 405
                                                 W+e+ie wk++++++++++ + ikPq vi++l +l + +aiv+tdvGqhqmw+aqf+k+++pr+ ++
  lcl|NCBI__GCF_000016745.1:WP_011940524.1 347 VpWTEEIEGWKAKHPMSYKQSATVIKPQFVIQKLRELSDPDAIVATDVGQHQMWTAQFFKFNRPRTLLS 415
                                               46******************************************************************* PP

                                 TIGR00118 406 sgGlGtmGfGlPaalGakvakpeetvvavtGdgsfqmnlqelstiveydipvkivilnnellGmvkqWq 474
                                               sgGlGtmG+GlPaa+Ga+ a p+ +v+ ++Gdg+fqmnlqel+t+v+  + vki+i+nn++lGmv+qWq
  lcl|NCBI__GCF_000016745.1:WP_011940524.1 416 SGGLGTMGYGLPAAMGAQAAFPDRQVIVICGDGGFQMNLQELATVVQNRLAVKICIINNNFLGMVRQWQ 484
                                               ********************************************************************* PP

                                 TIGR00118 475 elfyeerysetklaselpdfvklaeayGvkgiriekpeeleeklkealeskepvlldvevdkeeevlPm 543
                                               elf+++rys+t+++    df+klaea+G+kg + +kp+e+e+ +k+ +++ +pv+++++v++ee+vlPm
  lcl|NCBI__GCF_000016745.1:WP_011940524.1 485 ELFFDKRYSQTCMEL-PIDFIKLAEAFGAKGFQATKPDEVEAVIKKGFATPGPVIMEFKVAREEKVLPM 552
                                               **************6.88*************************************************** PP

                                 TIGR00118 544 vapGagldelve 555
                                               v+ Ga+l+e+v 
  lcl|NCBI__GCF_000016745.1:WP_011940524.1 553 VPAGASLTEMVL 564
                                               **********96 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (557 nodes)
Target sequences:                          1  (566 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.03u 0.01s 00:00:00.04 Elapsed: 00:00:00.04
# Mc/sec: 7.79
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis