Alignments for a candidate for dapF in Methanococcus aeolicus Nankai-3

GapMind for Amino acid biosynthesis

Alignments for a candidate for dapF in Methanococcus aeolicus Nankai-3

Align Diaminopimelate epimerase; DAP epimerase; EC 5.1.1.7; PLP-independent amino acid racemase (uncharacterized)
to candidate WP_011972928.1 MAEO_RS01020 diaminopimelate epimerase

Query= curated2:Q58519
         (295 letters)



>NCBI__GCF_000017185.1:WP_011972928.1
          Length = 295

 Score =  468 bits (1203), Expect = e-137
 Identities = 218/290 (75%), Positives = 259/290 (89%)

Query: 1   MEFTKMHALGNDYIVINEFDGEKVKEEEKAEFSRKICRRGFSVGADGVIFIQKPTSDEYD 60
           + FTKMH LGNDYIVINEFDGEK+KE+EKAEFS+KIC+RGFS+GADGVIF+Q  T ++YD
Sbjct: 3   LNFTKMHGLGNDYIVINEFDGEKIKEDEKAEFSKKICKRGFSIGADGVIFVQPATDEKYD 62

Query: 61  VRFRIFNSDGSEAEMCGNGIRCFSKYVYERIMKKNPLKVETKGGLRVSEMEIEGDEVKKI 120
           +RFRIFNSDGSEAEMCGNGIRCFSK+VYER+M+KNPL VET GGLRVSEM IE + VK I
Sbjct: 63  IRFRIFNSDGSEAEMCGNGIRCFSKFVYERVMQKNPLNVETMGGLRVSEMNIENNIVKSI 122

Query: 121 KVYMGVPKFKLKDIPMVVDGYKEDDEFLNGELKLKNPYLPKVKLSVVNVGNPHAVIFVED 180
           KV+MG P F+LKDIPMVV+G  E+D FL+ E++LKN  L  VKLSVVNVGNPHAVIF++D
Sbjct: 123 KVFMGAPTFELKDIPMVVEGKSENDVFLDEEIELKNALLNSVKLSVVNVGNPHAVIFMKD 182

Query: 181 NNIDLDFVREHLDVIGKEIEHHEAFPERINVHFVKVLNPNEIRIVTWERGAGYTTACGTG 240
           NN+   F+R HL+++GKEIEHH  FPE+INVHFV+++NP EI+I+TWERGAGYTTACGTG
Sbjct: 183 NNLKAPFIRTHLNILGKEIEHHSVFPEKINVHFVEIVNPQEIKIITWERGAGYTTACGTG 242

Query: 241 TTASVIMAHKLGKTNNRVLAHLDGGDLEIEIKDDGVYMIGDAVMVYDAKL 290
           TT+SVI+A+KLGKT N+VLAHLDGGDLEIEIK+DGVYM GDAV+VYD +L
Sbjct: 243 TTSSVIIANKLGKTGNKVLAHLDGGDLEIEIKEDGVYMEGDAVIVYDGEL 292


Lambda     K      H
   0.319    0.140    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 450
Number of extensions: 16
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 295
Length of database: 295
Length adjustment: 26
Effective length of query: 269
Effective length of database: 269
Effective search space:    72361
Effective search space used:    72361
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

Align candidate WP_011972928.1 MAEO_RS01020 (diaminopimelate epimerase)
to HMM TIGR00652 (dapF: diaminopimelate epimerase (EC 5.1.1.7))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00652.hmm
# target sequence database:        /tmp/gapView.850.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00652  [M=270]
Accession:   TIGR00652
Description: DapF: diaminopimelate epimerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   7.5e-105  336.2   1.8   8.5e-105  336.0   1.8    1.0  1  lcl|NCBI__GCF_000017185.1:WP_011972928.1  MAEO_RS01020 diaminopimelate epi


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000017185.1:WP_011972928.1  MAEO_RS01020 diaminopimelate epimerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  336.0   1.8  8.5e-105  8.5e-105       2     269 ..       4     293 ..       3     294 .. 0.97

  Alignments for each domain:
  == domain 1  score: 336.0 bits;  conditional E-value: 8.5e-105
                                 TIGR00652   2 eFlkmhGlgNdFvlvdevdeelvkee..aelvrkvcdrhtgvgaDgvllvep.sseeadvklrifNsDG 67 
                                               +F+kmhGlgNd+++++e+d e++ke+  ae+ +k+c+r +++gaDgv++v+p ++e++d+++rifNsDG
  lcl|NCBI__GCF_000017185.1:WP_011972928.1   4 NFTKMHGLGNDYIVINEFDGEKIKEDekAEFSKKICKRGFSIGADGVIFVQPaTDEKYDIRFRIFNSDG 72 
                                               8**********************999899***********************89999************ PP

                                 TIGR00652  68 SeaemCGNgiRcfakfvyekglkekkelsvetlaglikveveeen...kkvkvdmgepkfkkeeipltv 133
                                               SeaemCGNgiRcf+kfvye+ ++ k+ l+vet  gl+++e++ en   k++kv mg p+f+ ++ip++v
  lcl|NCBI__GCF_000017185.1:WP_011972928.1  73 SEAEMCGNGIRCFSKFVYERVMQ-KNPLNVETMGGLRVSEMNIENnivKSIKVFMGAPTFELKDIPMVV 140
                                               ***********************.7*******************99999******************** PP

                                 TIGR00652 134 ekeeekeellalev.........l...vvdvGnPHlvvfved......vekldleelgklleaheefpe 184
                                               e+++e++++l  e+         +   vv+vGnPH+v+f++d      + + +l+ lgk++e+h  fpe
  lcl|NCBI__GCF_000017185.1:WP_011972928.1 141 EGKSENDVFLDEEIelknallnsVklsVVNVGNPHAVIFMKDnnlkapFIRTHLNILGKEIEHHSVFPE 209
                                               *********999989999887662668***************9999998899***************** PP

                                 TIGR00652 185 gvNvefvevkkedeiklrvyERGageTlaCGtGavAsavvalklgktkkkvtvhleggeLeievkedgk 253
                                               ++Nv+fve+++++eik+ ++ERGag+T+aCGtG++ s+++a+klgkt +kv  hl+gg+Leie+kedg 
  lcl|NCBI__GCF_000017185.1:WP_011972928.1 210 KINVHFVEIVNPQEIKIITWERGAGYTTACGTGTTSSVIIANKLGKTGNKVLAHLDGGDLEIEIKEDG- 277
                                               *******************************************************************8. PP

                                 TIGR00652 254 vyltGpavlvlegela 269
                                               vy++G+av v++gel+
  lcl|NCBI__GCF_000017185.1:WP_011972928.1 278 VYMEGDAVIVYDGELY 293
                                               *************986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (270 nodes)
Target sequences:                          1  (295 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 6.67
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis