Align Phosphoribosyl isomerase A; 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; EC 5.3.1.16; N-(5'-phosphoribosyl)anthranilate isomerase; PRAI; EC 5.3.1.24; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (uncharacterized)
to candidate WP_011973909.1 MAEO_RS06090 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase
Query= curated2:P60583 (243 letters) >NCBI__GCF_000017185.1:WP_011973909.1 Length = 241 Score = 130 bits (328), Expect = 2e-35 Identities = 80/244 (32%), Positives = 143/244 (58%), Gaps = 10/244 (4%) Query: 2 LILLPAVDVVDGRAVRLVQGKAGSE-TEYGSALDAALGWQRDGAEWIHLVDLDAA-FGRG 59 +I++PAVD+ + + V+L+QG + E + L+ A W + GAE IHLVDLD+A +G Sbjct: 1 MIVIPAVDIKNKKCVQLIQGNPDKKHIELDNPLEVAEKWIKCGAEMIHLVDLDSAIYGTD 60 Query: 60 SNRELLAEVVGKLDVRVELSGGIRDDDSLAAALATGCARVNLGTAALENPQWCARAI--G 117 +N+E + +++ + V VE+ GG+R + + G RV LGT+A++NP+ + G Sbjct: 61 TNKETIKKIIESVPVPVEVGGGVRTVEDALNLIDLGAERVILGTSAVKNPEIITELLEKG 120 Query: 118 EHGDKVAVGLDVQIIDGQHRLRGRGWETDGGDLWEVLERLERQGCSRYVVTDVTKDGTLG 177 + +K+ V LD + DG+ ++G +T+ + E+ + LE +G + T+V +G L Sbjct: 121 INSEKIMVALDAK--DGKVVIKGWMEKTEYTPI-EIGKILEEKGAGSILFTNVDVEGLLD 177 Query: 178 GPNLDLLGAVADRTDAPVIASGGVSSLDDLRAIATLTGRGVEGAIVGKALYAGRFTLPQA 237 G +++ + + D + P+IASGG+++ DDL + + GV+G +VG A+Y L +A Sbjct: 178 GIDIEPVKKLVDELNIPIIASGGITTYDDLLKLKEI---GVDGVVVGSAIYKEMMDLKKA 234 Query: 238 LAAV 241 + V Sbjct: 235 IKIV 238 Lambda K H 0.318 0.138 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 187 Number of extensions: 14 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 243 Length of database: 241 Length adjustment: 23 Effective length of query: 220 Effective length of database: 218 Effective search space: 47960 Effective search space used: 47960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory