Align isochorismate lyase (EC 4.2.99.21); isochorismate synthase (EC 5.4.4.2) (characterized)
to candidate WP_012101661.1 CKL_RS06270 anthranilate synthase component I
Query= BRENDA::P9WFX1 (450 letters) >NCBI__GCF_000016505.1:WP_012101661.1 Length = 476 Score = 119 bits (298), Expect = 2e-31 Identities = 78/256 (30%), Positives = 125/256 (48%), Gaps = 1/256 (0%) Query: 187 FRRRVAVAVDEIAAGRYHKVILSRCVEVPFAIDFPLTYRLGRRHNTPVRSFLLQLGGIRA 246 F R V A + I +G +V++S+ ++ I YR R N F + + Sbjct: 218 FCRNVEKAKEYIKSGDIFQVVISQRLKCSTEIKAFDIYRKLRYENPSSYLFYMDFKDFQI 277 Query: 247 LGYSPELVTAVRADGVVITEPLAGTRALGRGPAIDRLARDDLESNSKEIVEHAISVRSSL 306 +G SPE + +V D V T P+AGTR G D +++L ++ KE EH + V Sbjct: 278 VGSSPERLVSVYGDRVS-TNPIAGTRKRGTSREEDEKLKNELINDEKERAEHVMLVDLGR 336 Query: 307 EEITDIAEPGSAAVIDFMTVRERGSVQHLGSTIRARLDPSSDRMAALEALFPAVTASGIP 366 +I ++E G+ V FM V++ + HL S + +L A + PA T SG P Sbjct: 337 NDIGKVSEFGTVEVTKFMEVQKYSHIMHLVSEVSGKLKSGISSFDAFISCLPAGTVSGAP 396 Query: 367 KAAGVEAIFRLDECPRGLYSGAVVMLSADGGLDAALTLRAAYQVGGRTWLRAGAGIIEES 426 K +E I +++ R LY+GAV S + +D + +R G +++AG GI+ +S Sbjct: 397 KIRAMEIIDEIEDRKRLLYAGAVGYFSYNKNMDTCIAIRTVVIKEGYAYMQAGGGIVYDS 456 Query: 427 EPEREFEETCEKLSTL 442 +PE E+EE+ K L Sbjct: 457 DPESEYEESLNKAKAL 472 Lambda K H 0.319 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 319 Number of extensions: 14 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 450 Length of database: 476 Length adjustment: 33 Effective length of query: 417 Effective length of database: 443 Effective search space: 184731 Effective search space used: 184731 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory