Align Anthranilate synthase component 2; AS; ASII; EC 4.1.3.27; Anthranilate synthase, GATase component; Anthranilate synthase, glutamine amidotransferase component (uncharacterized)
to candidate WP_012102720.1 CKL_RS11630 glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit
Query= curated2:Q57690 (197 letters) >NCBI__GCF_000016505.1:WP_012102720.1 Length = 354 Score = 82.4 bits (202), Expect = 9e-21 Identities = 55/178 (30%), Positives = 88/178 (49%), Gaps = 13/178 (7%) Query: 18 NLVQYVGTLGYKVKLVDNKITLDEIKKINPDRIIISPGPKTPKEAGNCIKIIQEV--DIP 75 N+++ + + + + + + I INP I++S GP PK+ + + +I+++ +P Sbjct: 181 NIIENLKSRNCDITIFPYNTSYENIMSINPKGILLSNGPGDPKDVYDVVGVIKKMISTVP 240 Query: 76 ILGVCLGHQCIVEAFGGEVGRAKRVMHGKASLINHDGEGIF-KDIPNPFYGGRYHSLIAK 134 I G+CLGHQ + A GG+ + K G NH G++ D F + H K Sbjct: 241 IFGICLGHQLLSLALGGDTYKMKFGHRGG----NH---GVYDSDTDKSFITSQNHGYAVK 293 Query: 135 EVPKE---LKITAKSLDDNYIMGVRHKKLPIEGVQFHPESILTESDNLKFPDLGLKLI 189 + +KIT +L+DN + G RHK LPI VQFHPE +D D L L+ Sbjct: 294 RDSLDKNNIKITHVNLNDNTVEGFRHKTLPIFSVQFHPEGSPGPTDTSYLFDKFLSLM 351 Lambda K H 0.319 0.142 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 207 Number of extensions: 13 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 197 Length of database: 354 Length adjustment: 25 Effective length of query: 172 Effective length of database: 329 Effective search space: 56588 Effective search space used: 56588 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory