Alignments for a candidate for tpiA in Clostridium kluyveri DSM 555

GapMind for Amino acid biosynthesis

Alignments for a candidate for tpiA in Clostridium kluyveri DSM 555

Align Triosephosphate isomerase; TIM; TPI; Triose-phosphate isomerase; EC 5.3.1.1 (characterized)
to candidate WP_012103713.1 CKL_RS16440 triose-phosphate isomerase

Query= SwissProt::Q8XKU1
         (248 letters)



>NCBI__GCF_000016505.1:WP_012103713.1
          Length = 248

 Score =  345 bits (886), Expect = e-100
 Identities = 167/248 (67%), Positives = 204/248 (82%)

Query: 1   MRTPIIAGNWKMHYTIDEAVKLVEELKPLVKDAKCEVVVCPTFVCLDAVKKAVEGTNIKV 60
           MR  IIAGNWKM+  +++A++LVEELKPLV+ AKC+VV+CP +VCLDAV K+V GTNIKV
Sbjct: 1   MRKAIIAGNWKMNKNLEDALELVEELKPLVRGAKCDVVLCPPYVCLDAVVKSVGGTNIKV 60

Query: 61  GAQNMHFEEKGAFTGEIAPRMLEAMNIDYVIIGHSERREYFNETDETCNKKVKAAFAHNL 120
           GAQNMH+EE GA+TGEI+P ML+++ +DYVIIGHSERR+YFNE DET NKK+K AF H++
Sbjct: 61  GAQNMHYEESGAYTGEISPGMLKSLKVDYVIIGHSERRQYFNEKDETINKKIKKAFEHDI 120

Query: 121 TPILCCGETLEQRENGTTNDVIKAQITADLEGLTKEQAEKVVIAYEPIWAIGTGKTATSD 180
            PI+CCGE+L +RENG T +V+  QI   L+ L KEQ EK+VIAYEPIWAIGTGKTAT  
Sbjct: 121 IPIVCCGESLLERENGITEEVLGRQIKLALKDLRKEQVEKIVIAYEPIWAIGTGKTATDK 180

Query: 181 QANETIAAIRAMVAEMFGQEVADKVRIQYGGSVKPNTIAEQMAKSDIDGALVGGASLVAA 240
           QAN+TIA IR +V++M+G+  A+ VRIQYGGSVKP TI  QM +  IDGALVGGASL   
Sbjct: 181 QANDTIAHIRGVVSKMYGENAAEVVRIQYGGSVKPATIKAQMEQPHIDGALVGGASLKPQ 240

Query: 241 DFAQIVNY 248
           DFA IVNY
Sbjct: 241 DFAAIVNY 248


Lambda     K      H
   0.316    0.131    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 218
Number of extensions: 5
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 248
Length of database: 248
Length adjustment: 24
Effective length of query: 224
Effective length of database: 224
Effective search space:    50176
Effective search space used:    50176
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 46 (22.3 bits)

Align candidate WP_012103713.1 CKL_RS16440 (triose-phosphate isomerase)
to HMM TIGR00419 (tpiA: triose-phosphate isomerase (EC 5.3.1.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00419.hmm
# target sequence database:        /tmp/gapView.5801.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00419  [M=228]
Accession:   TIGR00419
Description: tim: triose-phosphate isomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    5.2e-59  185.8   0.8      6e-59  185.6   0.8    1.0  1  lcl|NCBI__GCF_000016505.1:WP_012103713.1  CKL_RS16440 triose-phosphate iso


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000016505.1:WP_012103713.1  CKL_RS16440 triose-phosphate isomerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  185.6   0.8     6e-59     6e-59       1     227 [.       5     239 ..       5     240 .. 0.93

  Alignments for each domain:
  == domain 1  score: 185.6 bits;  conditional E-value: 6e-59
                                 TIGR00419   1 lviinfKlnesvgkvelevaklaeevaseagvevavappfvdldvvkdeve.seiqvaAqnvdavksGa 68 
                                               ++ +n+K+n  +++  ++v +l+  v   a++ v++ pp+v ld v++ v  ++i+v+Aqn++  +sGa
  lcl|NCBI__GCF_000016505.1:WP_012103713.1   5 IIAGNWKMNKNLEDALELVEELKPLV-RGAKCDVVLCPPYVCLDAVVKSVGgTNIKVGAQNMHYEESGA 72 
                                               6899***************9998655.6689*******************999**************** PP

                                 TIGR00419  69 ftGeisAemlkdlGakgvligHsErRsllkeadeliekkvarlkelglksvvCvgetleereaartinn 137
                                               +tGeis  mlk l + +v+igHsErR +++e de i+kk+ ++ e ++ ++vC ge+l ere + t ++
  lcl|NCBI__GCF_000016505.1:WP_012103713.1  73 YTGEISPGMLKSLKVDYVIIGHSERRQYFNEKDETINKKIKKAFEHDIIPIVCCGESLLERENGITEEV 141
                                               **************************************************************6666665 PP

                                 TIGR00419 138 vattaaaaA.......lepdvvAvEPveliGtGkpvskAeaevveksvrdhlkk.vskevaesvrvlyG 198
                                               + ++ + a         e+ v+A+EP+++iGtGk+++  +a+   + +r  ++k   ++ ae vr++yG
  lcl|NCBI__GCF_000016505.1:WP_012103713.1 142 LGRQIKLALkdlrkeqVEKIVIAYEPIWAIGTGKTATDKQANDTIAHIRGVVSKmYGENAAEVVRIQYG 210
                                               5555555545666666************************************9977889999******* PP

                                 TIGR00419 199 asvtaaedaelaaqldvdGvLlasavlka 227
                                               +sv+ a  + ++ q+ +dG+L+++a+lk+
  lcl|NCBI__GCF_000016505.1:WP_012103713.1 211 GSVKPATIKAQMEQPHIDGALVGGASLKP 239
                                               ****************************8 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (228 nodes)
Target sequences:                          1  (248 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 6.34
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis