Alignments for a candidate for carA in Azorhizobium caulinodans ORS 571

GapMind for Amino acid biosynthesis

Alignments for a candidate for carA in Azorhizobium caulinodans ORS 571

Align Carbamoyl-phosphate synthase small chain; Carbamoyl-phosphate synthetase glutamine chain; EC 6.3.5.5 (characterized)
to candidate WP_012172161.1 AZC_RS18745 carbamoyl-phosphate synthase small subunit

Query= SwissProt::P0A6F1
         (382 letters)



>NCBI__GCF_000010525.1:WP_012172161.1
          Length = 401

 Score =  380 bits (977), Expect = e-110
 Identities = 198/387 (51%), Positives = 255/387 (65%), Gaps = 14/387 (3%)

Query: 4   SALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNV 63
           +ALLVL DGT   G   GA    VGEV FNT++TGY+EILTDPSY+ QI+T T+PHIGNV
Sbjct: 20  TALLVLADGTVLEGFGFGAATEGVGEVCFNTAITGYEEILTDPSYAGQIITFTFPHIGNV 79

Query: 64  GTNDADEESSQVHA----QGLVIRDLPLIASNFRNTEDLSSYLKRHNIVAIADIDTRKLT 119
           GTN+ D E+    A    +G+V+R      SN+R T  L ++LK  +I AI+ IDTR LT
Sbjct: 80  GTNEEDIETVSAAAVSGVRGVVVRGSVTDPSNYRATRHLDAWLKARDIPAISGIDTRALT 139

Query: 120 RLLREKGAQNGCIIAGDNPDAALALE----KARAFPGLNGMDLAKEVTTAEAYSWTQGSW 175
            L+RE G  N   +   NP     +E    KA+ +PGL GMDL   VT+ + ++W +  W
Sbjct: 140 ALIREGGMPNA--VLAHNPAGVFDIEALKAKAKEWPGLVGMDLVPGVTSVQRFNWDETEW 197

Query: 176 TLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTIVPAQTSAEDVLKMNPDG 235
              GG      +    +HVVA D+G KRNILR+L   GC++T+VPA TSA DVL + PDG
Sbjct: 198 QWNGGYGH---QGAPKYHVVAIDYGVKRNILRLLATAGCKVTVVPATTSAADVLALQPDG 254

Query: 236 IFLSNGPGDPAPC-DYAITAIQKFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGN 294
           +FLSNGPGDPA   +YA+  I++ +  +IP FGICLGHQ++ LA G +T+KM  GHHG N
Sbjct: 255 VFLSNGPGDPAATGEYAVPVIRELIAKEIPTFGICLGHQMIGLAVGGRTLKMHQGHHGAN 314

Query: 295 HPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFDGTLQGIHRTDKPAFSFQGHP 354
           HPVKD+    V IT+ NHGFAVD  +LPAN + TH SLFDG+  GI   DKP FS Q HP
Sbjct: 315 HPVKDLTTGKVEITSMNHGFAVDRDSLPANAQETHVSLFDGSNAGIALADKPVFSVQYHP 374

Query: 355 EASPGPHDAAPLFDHFIELIEQYRKTA 381
           EASPGP D+  LF  F+EL+ +++  A
Sbjct: 375 EASPGPRDSHYLFTRFVELMAKHKTAA 401


Lambda     K      H
   0.318    0.135    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 498
Number of extensions: 26
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 382
Length of database: 401
Length adjustment: 31
Effective length of query: 351
Effective length of database: 370
Effective search space:   129870
Effective search space used:   129870
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate WP_012172161.1 AZC_RS18745 (carbamoyl-phosphate synthase small subunit)
to HMM TIGR01368 (carA: carbamoyl-phosphate synthase, small subunit (EC 6.3.5.5))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01368.hmm
# target sequence database:        /tmp/gapView.1046.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01368  [M=361]
Accession:   TIGR01368
Description: CPSaseIIsmall: carbamoyl-phosphate synthase, small subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.2e-137  444.6   0.0   1.4e-137  444.4   0.0    1.0  1  lcl|NCBI__GCF_000010525.1:WP_012172161.1  AZC_RS18745 carbamoyl-phosphate 


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000010525.1:WP_012172161.1  AZC_RS18745 carbamoyl-phosphate synthase small subunit
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  444.4   0.0  1.4e-137  1.4e-137       1     360 [.      21     395 ..      21     396 .. 0.94

  Alignments for each domain:
  == domain 1  score: 444.4 bits;  conditional E-value: 1.4e-137
                                 TIGR01368   1 atlvledGtvfegksfgaekevvGevvFnTsmtGYqEiltDpsYkgqivvltyplignygvneedaesk 69 
                                               a lvl+dGtv+eg +fga +e vGev+FnT++tGY+EiltDpsY+gqi+++t p+ign+g+need+e  
  lcl|NCBI__GCF_000010525.1:WP_012172161.1  21 ALLVLADGTVLEGFGFGAATEGVGEVCFNTAITGYEEILTDPSYAGQIITFTFPHIGNVGTNEEDIETV 89 
                                               579***************************************************************865 PP

                                 TIGR01368  70 ....kikvkglvvkelskevsnyrakesLeeflkeegivaiegvDTRalvkklRekgsmkavistekse 134
                                                      v+g+vv+   +++snyra++ L+++lk ++i+ai g+DTRal+  +Re g  +av++++   
  lcl|NCBI__GCF_000010525.1:WP_012172161.1  90 saaaVSGVRGVVVRGSVTDPSNYRATRHLDAWLKARDIPAISGIDTRALTALIREGGMPNAVLAHNPAG 158
                                               222244689********************************************************9976 PP

                                 TIGR01368 135 ...keelvekakespkvkevnlvkevstkeayeleq...k.....akkegkklrvvvidlGvKenilre 192
                                                   e l++kake p + +++lv  v+  ++ ++ +   +      ++   k++vv+id+GvK+nilr 
  lcl|NCBI__GCF_000010525.1:WP_012172161.1 159 vfdIEALKAKAKEWPGLVGMDLVPGVTSVQRFNWDEtewQwnggyGHQGAPKYHVVAIDYGVKRNILRL 227
                                               6668999999************************9966515566445556669**************** PP

                                 TIGR01368 193 LvkrgvevtvvpadtsaeeikklnpdgillsnGPGdPaav.eeaietvkklleakiPifGIclGhqlla 260
                                               L++ g++vtvvpa+tsa+++ +l+pdg++lsnGPGdPaa  e+a+  +++l+ ++iP+fGIclGhq+++
  lcl|NCBI__GCF_000010525.1:WP_012172161.1 228 LATAGCKVTVVPATTSAADVLALQPDGVFLSNGPGDPAATgEYAVPVIRELIAKEIPTFGICLGHQMIG 296
                                               *************************************8763778999********************** PP

                                 TIGR01368 261 lalgaktyklkfGhrGaNhpvkdlktgrveitsqNHgyavdeeslkeeelevthvnlnDgtveglehke 329
                                               la+g++t+k++ Gh+GaNhpvkdl+tg+veits NHg+avd++sl+++ ++ thv+l+Dg+++g++  +
  lcl|NCBI__GCF_000010525.1:WP_012172161.1 297 LAVGGRTLKMHQGHHGANHPVKDLTTGKVEITSMNHGFAVDRDSLPAN-AQETHVSLFDGSNAGIALAD 364
                                               ********************************************9866.99****************** PP

                                 TIGR01368 330 lpvfsvQyHPeaspGphdteylFdefvelik 360
                                               +pvfsvQyHPeaspGp+d++ylF +fvel++
  lcl|NCBI__GCF_000010525.1:WP_012172161.1 365 KPVFSVQYHPEASPGPRDSHYLFTRFVELMA 395
                                               ****************************997 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (361 nodes)
Target sequences:                          1  (401 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01
# Mc/sec: 7.29
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis