Alignments for a candidate for hisD in Heliobacterium modesticaldum Ice1; ATCC 51547

GapMind for Amino acid biosynthesis

Alignments for a candidate for hisD in Heliobacterium modesticaldum Ice1; ATCC 51547

Align histidinol dehydrogenase (EC 1.1.1.23) (characterized)
to candidate WP_012284033.1 HM1_RS13880 histidinol dehydrogenase

Query= BRENDA::Q8G2R2
         (430 letters)



>NCBI__GCF_000019165.1:WP_012284033.1
          Length = 426

 Score =  367 bits (943), Expect = e-106
 Identities = 199/412 (48%), Positives = 270/412 (65%), Gaps = 2/412 (0%)

Query: 18  AFLSGKREVSEDVDRAVREIVDRVRREGDSALLDYSRRFDRIDLEKTGIAVTEAEIDAAF 77
           A+L  K      V   V E++  VR +G +AL  Y++RFD  D++++   V+EAEIDAA+
Sbjct: 14  AYLQKKSFDDVAVAERVAEVIANVRNQGTAALFAYTKRFDGADVDESNFRVSEAEIDAAY 73

Query: 78  DAAPASTVEALKLARDRIEKHHARQLPKDDRYTDALGVELGSRWTAIEAVGLYVPGGTAS 137
                S + AL+ A + I + H +QL       +A G  LG     +E VG+YVPGG AS
Sbjct: 74  SQVDPSVLAALRRACENIRRFHEKQLRPSWIEPEADGTMLGQLIRPLERVGVYVPGGLAS 133

Query: 138 YPSSVLMNAMPAKVAGVDRIVMVVP-APDGNLNPLVLVAARLAGVSEIYRVGGAQAIAAL 196
           YPSSVLMNA+PAKVAGV ++VM  P   DG++NP  LVAA+ AGV E+YR+GGAQA+AA+
Sbjct: 134 YPSSVLMNAVPAKVAGVPQVVMATPPGKDGSINPYSLVAAKEAGVDEVYRMGGAQAVAAM 193

Query: 197 AYGTETIRPVAKIVGPGNAYVAAAKRIVFGTVGIDMIAGPSEVLIVADKDNNPDWIAADL 256
           A+G   ++ V KI GPGN YV  AK+ V+GTV IDM+AGPSEVL++AD+   P ++AAD 
Sbjct: 194 AFGVG-LKAVDKITGPGNIYVTLAKKQVYGTVDIDMLAGPSEVLVIADESAAPAYVAADF 252

Query: 257 LAQAEHDTAAQSILMTNDEAFAHAVEEAVERQLHTLARTETASASWRDFGAVILVKDFED 316
           L+QAEHD  A ++L+T   A A AVE  + RQL TL R E    + RD GA+ +VKD E+
Sbjct: 253 LSQAEHDVRAATVLVTPSAALADAVEAEIARQLATLPRREIMEQALRDNGAIFIVKDLEE 312

Query: 317 AIPLANRIAAEHLEIAVADAEAFVPRIRNAGSIFIGGYTPEVIGDYVGGCNHVLPTARSA 376
           A  ++NR A EHLE+      A + ++  AG+IF+G Y+PE +GDY  G NHVLPT  +A
Sbjct: 313 ACQVSNRFAPEHLEVLTEAPFALLGKLTQAGAIFLGPYSPEPVGDYFAGPNHVLPTGGTA 372

Query: 377 RFSSGLSVLDYMKRTSLLKLGSEQLRALGPAAIEIARAEGLDAHAQSVAIRL 428
           RF S L+V  + K+TSL+     +        I +A+AEGLDAHA +VA+RL
Sbjct: 373 RFYSPLNVDTFQKKTSLIAYSKVRFDRDAKDIIALAKAEGLDAHANAVAVRL 424


Lambda     K      H
   0.318    0.134    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 476
Number of extensions: 13
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 430
Length of database: 426
Length adjustment: 32
Effective length of query: 398
Effective length of database: 394
Effective search space:   156812
Effective search space used:   156812
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

Align candidate WP_012284033.1 HM1_RS13880 (histidinol dehydrogenase)
to HMM TIGR00069 (hisD: histidinol dehydrogenase (EC 1.1.1.23))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00069.hmm
# target sequence database:        /tmp/gapView.25211.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00069  [M=393]
Accession:   TIGR00069
Description: hisD: histidinol dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   8.5e-173  561.0   0.9   9.7e-173  560.8   0.9    1.0  1  lcl|NCBI__GCF_000019165.1:WP_012284033.1  HM1_RS13880 histidinol dehydroge


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000019165.1:WP_012284033.1  HM1_RS13880 histidinol dehydrogenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  560.8   0.9  9.7e-173  9.7e-173       1     393 []      30     423 ..      30     423 .. 0.99

  Alignments for each domain:
  == domain 1  score: 560.8 bits;  conditional E-value: 9.7e-173
                                 TIGR00069   1 vkeiiedvrkeGdeAlleytekfdkv..kleslrvseeeleealeavdeelkealelaaeniekfhekq 67 
                                               v+e+i++vr++G +Al++yt++fd++  + +++rvse+e+++a+++vd+++ +al++a eni++fhekq
  lcl|NCBI__GCF_000019165.1:WP_012284033.1  30 VAEVIANVRNQGTAALFAYTKRFDGAdvDESNFRVSEAEIDAAYSQVDPSVLAALRRACENIRRFHEKQ 98 
                                               789*********************98667889************************************* PP

                                 TIGR00069  68 lpesveveteegvllgqkvrplervglYvPgGkaaypStvlmtavpAkvAgvkeivvvtPpkkdgkvnp 136
                                               l+ s+   +++g++lgq++rplervg+YvPgG a+ypS+vlm+avpAkvAgv+++v++tPp kdg++np
  lcl|NCBI__GCF_000019165.1:WP_012284033.1  99 LRPSWIEPEADGTMLGQLIRPLERVGVYVPGGLASYPSSVLMNAVPAKVAGVPQVVMATPPGKDGSINP 167
                                               ********************************************************************* PP

                                 TIGR00069 137 avlaaakllgvdevykvGGaqaiaalayGtetvpkvdkivGPGniyVtaAKklvfgevgidmiaGPsEv 205
                                                 l+aak +gvdevy++GGaqa+aa+a+G+  +++vdki+GPGniyVt AKk+v+g+v+idm+aGPsEv
  lcl|NCBI__GCF_000019165.1:WP_012284033.1 168 YSLVAAKEAGVDEVYRMGGAQAVAAMAFGV-GLKAVDKITGPGNIYVTLAKKQVYGTVDIDMLAGPSEV 235
                                               ******************************.9************************************* PP

                                 TIGR00069 206 lviadesanpelvaaDllsqaEHdedaqailvttseelaekveeeveeqleelerkeiaekslekngai 274
                                               lviadesa p++vaaD+lsqaEHd +a+++lvt+s++la++ve+e+++ql++l+r+ei+e++l++ngai
  lcl|NCBI__GCF_000019165.1:WP_012284033.1 236 LVIADESAAPAYVAADFLSQAEHDVRAATVLVTPSAALADAVEAEIARQLATLPRREIMEQALRDNGAI 304
                                               ********************************************************************* PP

                                 TIGR00069 275 ilvddleealelsneyApEHLelqtkdpeellkkiknaGsvflGeytpealgdyvaGpnhvLPTsgtAr 343
                                               ++v+dleea+++sn++ApEHLe+ t+ p +ll k+++aG++flG+y+pe++gdy+aGpnhvLPT+gtAr
  lcl|NCBI__GCF_000019165.1:WP_012284033.1 305 FIVKDLEEACQVSNRFAPEHLEVLTEAPFALLGKLTQAGAIFLGPYSPEPVGDYFAGPNHVLPTGGTAR 373
                                               ********************************************************************* PP

                                 TIGR00069 344 fasglsvedFlkrisvqelskealeelaeaveklaeaEgLeaHaeavevR 393
                                               f+s+l+v++F+k++s++ +sk  +++ a+++++la+aEgL+aHa+av+vR
  lcl|NCBI__GCF_000019165.1:WP_012284033.1 374 FYSPLNVDTFQKKTSLIAYSKVRFDRDAKDIIALAKAEGLDAHANAVAVR 423
                                               ***********************************************998 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (393 nodes)
Target sequences:                          1  (426 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 13.36
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis