Alignments for a candidate for thrC in Methylobacterium sp. 4-46

GapMind for Amino acid biosynthesis

Alignments for a candidate for thrC in Methylobacterium sp. 4-46

Align threonine synthase (EC 4.2.3.1) (characterized)
to candidate WP_012332209.1 M446_RS11150 threonine synthase

Query= BRENDA::Q8YFS0
         (463 letters)



>NCBI__GCF_000019365.1:WP_012332209.1
          Length = 471

 Score =  560 bits (1444), Expect = e-164
 Identities = 289/464 (62%), Positives = 338/464 (72%), Gaps = 2/464 (0%)

Query: 1   MKYVSTRGEAPVLGFSDALLAGLARDGGLYLPQEYPQFTAEQIRALRGKSYVEVALAVLT 60
           M +VSTRG AP L F+DALLAGLARDGGLYLP+ +P    E I  L GK Y E A AVL 
Sbjct: 1   MLHVSTRGAAPPLTFTDALLAGLARDGGLYLPESWPSLAPETIAGLAGKPYAEAAKAVLA 60

Query: 61  PFTGGEIPAADFERMVREAYGTFRHDAVCPLVQTDANEFVLELFHGPTLAFKDVAMQLLA 120
           P   GEIP A  +RM+  AY  FRH AVCPLVQ DAN F+LEL HGPTLAFKDVAMQLL 
Sbjct: 61  PLVDGEIPRAALDRMIEAAYAGFRHPAVCPLVQLDANLFLLELHHGPTLAFKDVAMQLLG 120

Query: 121 RMMDYVLAQRGERATIVGATSGDTGGAAIEAFGGRDNTDIFILFPNGRVSPVQQRQMTSS 180
           R+MD+VL  RG RATIVGATSGDTG AA+EAF G D  D+FILFP+GRVS VQ+RQMTS 
Sbjct: 121 RLMDHVLKARGSRATIVGATSGDTGSAAVEAFRGLDQVDVFILFPHGRVSEVQRRQMTSV 180

Query: 181 GFSNVHALSIEGNFDDCQNLVKGMFNDLEFCDALSLSGVNSINWARIMPQVVYYFTAALS 240
              NVHAL++EG FDDCQ +VK +F    F + + LSGVNSINWAR+  Q  YYFT+A++
Sbjct: 181 DSPNVHALAVEGTFDDCQAIVKALFQHPRFAEEVRLSGVNSINWARVAAQATYYFTSAVA 240

Query: 241 LGAPDRAVSFTVPTGNFGDIFAGYVAKRMGLPIEQLIIATNDNDILSRTLESGAYEMRGV 300
           LGAP R VSF VPTGNFGDI AG+VAKRMGLP+ +L+I TN NDIL+RTL +G YE+RGV
Sbjct: 241 LGAPHRPVSFAVPTGNFGDILAGWVAKRMGLPVGRLMIGTNANDILARTLATGTYEVRGV 300

Query: 301 AQTTSPSMDIQISSNFERLLFEAHGRDAAAVRGLMQGLKQSGGFTISEKPLSAIRSEFSA 360
           A TTSPSMDIQ+SSNFERLLFEA GR+   +  LM GLKQSG FT+  + L  +RSEF A
Sbjct: 301 APTTSPSMDIQVSSNFERLLFEALGREPEGLTRLMAGLKQSGRFTLPPEVLKRVRSEFDA 360

Query: 361 GRSTVDETAATIESVLSKDGYLLDPHSAIGVKVARE--KASGTAPMVVLATAHPAKFPDA 418
                 E AA I    ++ G +LDPHSAIGV+  R   +A    P+V LATAH AKFPDA
Sbjct: 361 AAVPEPEVAAEIRDTYARTGQILDPHSAIGVRAGRRLLEADPATPVVALATAHAAKFPDA 420

Query: 419 VKAACGVEPQLPAWLCDLMQRKESFTVLHNELKIVEEYVRHHSR 462
           V+AA G+ P LP  L DLM R E F+V+ N+   VE  +R  +R
Sbjct: 421 VEAATGIRPALPPHLADLMTRPERFSVVANDEGAVERVIRERAR 464


Lambda     K      H
   0.320    0.135    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 579
Number of extensions: 26
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 463
Length of database: 471
Length adjustment: 33
Effective length of query: 430
Effective length of database: 438
Effective search space:   188340
Effective search space used:   188340
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

Align candidate WP_012332209.1 M446_RS11150 (threonine synthase)
to HMM TIGR00260 (thrC: threonine synthase (EC 4.2.3.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00260.hmm
# target sequence database:        /tmp/gapView.13732.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00260  [M=340]
Accession:   TIGR00260
Description: thrC: threonine synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    5.6e-84  268.1   0.0    7.6e-84  267.7   0.0    1.1  1  lcl|NCBI__GCF_000019365.1:WP_012332209.1  M446_RS11150 threonine synthase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000019365.1:WP_012332209.1  M446_RS11150 threonine synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  267.7   0.0   7.6e-84   7.6e-84      15     334 ..      72     427 ..      64     432 .. 0.92

  Alignments for each domain:
  == domain 1  score: 267.7 bits;  conditional E-value: 7.6e-84
                                 TIGR00260  15 lvdlaegstelfrspklaeevga.enlyvkelfhgPtlaFKDrglqfvavlltkalelgne..tvlcAt 80 
                                               l  ++e  ++ fr p++   v++  nl+++el hgPtlaFKD+++q ++ l+ ++l++     t+++At
  lcl|NCBI__GCF_000019365.1:WP_012332209.1  72 LDRMIEAAYAGFRHPAVCPLVQLdANLFLLELHHGPTLAFKDVAMQLLGRLMDHVLKARGSraTIVGAT 140
                                               55678889999******98888888*****************************987654446****** PP

                                 TIGR00260  81 sGdtgaaaaealagkanvkvvvLyPkgkispv.keklvtalaenakvlaikGdFDdaqdlvkeifedke 148
                                               sGdtg+aa+ea++g   v+v++L P+g++s v   +++ +   n++ la++G+FDd+q++vk++f++ +
  lcl|NCBI__GCF_000019365.1:WP_012332209.1 141 SGDTGSAAVEAFRGLDQVDVFILFPHGRVSEVqRRQMTSVDSPNVHALAVEGTFDDCQAIVKALFQHPR 209
                                               ********************************999******************************9555 PP

                                 TIGR00260 149 ...klklnsvNsinparieaqktyafeiveqlgkespdkvvvpvpsgnfgailkGflekkelglpiekl 214
                                                  ++ l+ vNsin+ar++aq ty+f  + +lg      v ++vp gnfg+il+G+++k+++ lp+  l
  lcl|NCBI__GCF_000019365.1:WP_012332209.1 210 faeEVRLSGVNSINWARVAAQATYYFTSAVALG-APHRPVSFAVPTGNFGDILAGWVAKRMG-LPVGRL 276
                                               5669*****************************.66778*****************999999.****** PP

                                 TIGR00260 215 aiaaegaadivrrflksgdlepkedkeTlstAmdignpsnverale.larrslgnledlke........ 274
                                                i ++ + di+ r l +g  e +    T s++mdi  +sn+er+l+ +  r+ + l+ l +        
  lcl|NCBI__GCF_000019365.1:WP_012332209.1 277 MIGTNAN-DILARTLATGTYEVRGVAPTTSPSMDIQVSSNFERLLFeALGREPEGLTRLMAglkqsgrf 344
                                               ****998.**********666666669******************99888888888888779999**** PP

                                 TIGR00260 275 .................svsdeeileaikklaeeegyllephtavavaalkklvekg....vs..atad 320
                                                                +v + e+  +i+  +++ g +l+ph+a++v+a ++l+e++    v   ata+
  lcl|NCBI__GCF_000019365.1:WP_012332209.1 345 tlppevlkrvrsefdaaAVPEPEVAAEIRDTYARTGQILDPHSAIGVRAGRRLLEADpatpVValATAH 413
                                               ****************999**************************************97652268**** PP

                                 TIGR00260 321 paKFeevvealtgn 334
                                                aKF+++vea tg 
  lcl|NCBI__GCF_000019365.1:WP_012332209.1 414 AAKFPDAVEAATGI 427
                                               **********9996 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (340 nodes)
Target sequences:                          1  (471 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01
# Mc/sec: 8.50
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis