Alignments for a candidate for metB in Methylobacterium sp. 4-46

GapMind for Amino acid biosynthesis

Alignments for a candidate for metB in Methylobacterium sp. 4-46

Align O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) (characterized)
to candidate WP_012334051.1 M446_RS20655 O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase

Query= reanno::HerbieS:HSERO_RS16440
         (413 letters)



>NCBI__GCF_000019365.1:WP_012334051.1
          Length = 432

 Score =  217 bits (553), Expect = 5e-61
 Identities = 138/418 (33%), Positives = 213/418 (50%), Gaps = 15/418 (3%)

Query: 11  TTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRYGRQGNPTVAALE 70
           T  +H+  +      ++  PI+ +  + ++ A   A +F  + PGYRY R  NPT A LE
Sbjct: 5   TIAIHAGYEDDPTTKAVAVPIYQTAAYAFDSAEHGAALFNLEMPGYRYSRIANPTNAVLE 64

Query: 71  DKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNSLWMTV-GAQGAK 129
            ++ ++E G + +  ATG AA+   +  L   G ++VS   L+G T++L+  V   QG  
Sbjct: 65  RRVAELEGGVAALSVATGQAALHYAIATLADCGGNIVSVPQLYGTTHTLFAHVLPRQGIH 124

Query: 130 VSMVDATDVKNVEAAITANTRLVFVETIANPRTQVADLKRIGELCRERGILYVVDNTMTS 189
                +   + V   I A+TR V+ E+I NP   + D+  + E+    G+  VVDNT+ +
Sbjct: 125 TRFAASDAPEAVARLIDADTRAVYCESIGNPAGNICDIAALAEVAHAHGVPLVVDNTVAT 184

Query: 190 PYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGEFDWTRYPHIAENYKKNPAPQWG 249
           P L RP   GA +V+ SLTK +GGHG  LGG + D G F W  +P     + K  A   G
Sbjct: 185 PILMRPLDHGADIVIASLTKFMGGHGTTLGGIIVDGGRFPWLAHPERFPMFCKPDASYHG 244

Query: 250 MA------------QIRAKALRDFGGSLGPEAAHHIAVGAETIALRQERECKNALALAQM 297
           +             + R+   R  G  L P +A  +  G ET+ALR ER  +NA  +A+ 
Sbjct: 245 LVYAERFGASAFAERARSVYQRTTGAVLAPFSAFLLLQGIETVALRVERHVENARKVAEF 304

Query: 298 LQADERVAAVYYPGLESHPQHALS-KALFRSFGSLMSFELKDG-IDCFDYLNRLRLAIPT 355
           L+ D RVA V Y G    P + ++ K L     SL++F ++ G      + + L L    
Sbjct: 305 LRDDPRVAWVNYAGFPDSPHYPMARKYLGGRASSLLTFGVQGGYAGGAAFYDALTLVKRL 364

Query: 356 SNLGDTRTLVIPVAHTIFYEMGAERRASMGIAESLIRVSVGLEDTDDLVADFRQALDA 413
            N+GD ++L    A T   +M  E +   G+    IR+SVG+E +DD++AD  QAL+A
Sbjct: 365 VNIGDAKSLACHPASTTHRQMSPEEQRHAGVLPEAIRLSVGIEHSDDILADLDQALEA 422


Lambda     K      H
   0.319    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 462
Number of extensions: 23
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 413
Length of database: 432
Length adjustment: 32
Effective length of query: 381
Effective length of database: 400
Effective search space:   152400
Effective search space used:   152400
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis