Alignments for a candidate for hom_kinase in Methylobacterium sp. 4-46

GapMind for Amino acid biosynthesis

Alignments for a candidate for hom_kinase in Methylobacterium sp. 4-46

Align homoserine kinase (EC 2.7.1.39) (characterized)
to candidate WP_012335681.1 M446_RS29110 homoserine kinase

Query= reanno::Korea:Ga0059261_3253
         (319 letters)



>NCBI__GCF_000019365.1:WP_012335681.1
          Length = 321

 Score =  300 bits (767), Expect = 4e-86
 Identities = 156/307 (50%), Positives = 200/307 (65%), Gaps = 2/307 (0%)

Query: 1   MAVYTQVSAEALAGFLARYDVGELVSAKGIAEGVENSNYLVETTRDRFILTLYEKRVEAA 60
           MAVYT+V  EALA FLA YD+G L+S KGIAEGVEN+N+ + TT   +ILTLYEKRV   
Sbjct: 1   MAVYTEVPDEALAAFLADYDIGGLLSYKGIAEGVENTNFYLHTTVGSYILTLYEKRVAEG 60

Query: 61  DLPYFMGLLDHLAAKGLPVPPAIKDRGGVEIQELNGRPACLIKFLSGISLSHPTPAQARA 120
           DLP+F+GL++HLAA+GL  P  I++R G  +  L GRPA ++ FL G+S+  P     RA
Sbjct: 61  DLPFFLGLMEHLAARGLACPQPIRNRAGTALGRLCGRPAVIVSFLDGVSVRRPGVRHCRA 120

Query: 121 AGEAMAQMHRAVADFPLDRPNSMGVDTWQPLFEKCGHSLDQIVPGLYDDLGFAIARVVPA 180
            G A+A +H A  DFP+ RPN++ V  W+PLF +     D++ P L       +AR+  A
Sbjct: 121 LGRALAGLHAAGVDFPMRRPNALSVAAWRPLFAQAEAQADRVAPNLAARTRDDLARLEEA 180

Query: 181 WTRNDFDRCAIHADLFPDNVLMRGDQVTGLIDFYFACTDIRVYDLAVMHSAWSFDAHGRN 240
           W R D     IHADLF DNV   GD V+GLIDFYFACTD   YDLA+  +AW F+  G +
Sbjct: 181 WPR-DLPGGVIHADLFTDNVFFIGDAVSGLIDFYFACTDAFAYDLAICLNAWCFELDG-S 238

Query: 241 YDPAVGDALIAGYEASFPLTEVERAAFPTLAAGACIRFSLSRAWDWLNTPADALVMRKDP 300
           ++   G A+IA Y+A+ PL   E  + P LA GA +RF L+R  DWLN P  ALV  KDP
Sbjct: 239 FNRTKGQAMIAAYQAARPLDPREVESLPLLARGAALRFMLTRLVDWLNVPPGALVKPKDP 298

Query: 301 LAYVRRL 307
           L Y R+L
Sbjct: 299 LEYDRKL 305


Lambda     K      H
   0.323    0.138    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 336
Number of extensions: 12
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 319
Length of database: 321
Length adjustment: 28
Effective length of query: 291
Effective length of database: 293
Effective search space:    85263
Effective search space used:    85263
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 48 (23.1 bits)

Align candidate WP_012335681.1 M446_RS29110 (homoserine kinase)
to HMM TIGR00938 (thrB: homoserine kinase (EC 2.7.1.39))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00938.hmm
# target sequence database:        /tmp/gapView.30579.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00938  [M=307]
Accession:   TIGR00938
Description: thrB_alt: homoserine kinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.2e-124  401.6   0.0   1.4e-124  401.4   0.0    1.0  1  lcl|NCBI__GCF_000019365.1:WP_012335681.1  M446_RS29110 homoserine kinase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000019365.1:WP_012335681.1  M446_RS29110 homoserine kinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  401.4   0.0  1.4e-124  1.4e-124       1     306 [.       1     306 [.       1     307 [. 0.99

  Alignments for each domain:
  == domain 1  score: 401.4 bits;  conditional E-value: 1.4e-124
                                 TIGR00938   1 mavytsvsdeeleafLegydlGellslkGiaeGvensnyllttdkgryvLtlyekrvkaeeLPfflell 69 
                                               mavyt+v de l afL  yd+G lls kGiaeGven+n++l t++g+y+Ltlyekrv + +LPffl l+
  lcl|NCBI__GCF_000019365.1:WP_012335681.1   1 MAVYTEVPDEALAAFLADYDIGGLLSYKGIAEGVENTNFYLHTTVGSYILTLYEKRVAEGDLPFFLGLM 69 
                                               9********************************************************999********* PP

                                 TIGR00938  70 thLaerglpvakpvksrdGralseLaGkPaalvefLkGssvakPtaercrevgevlaklhlagadfkee 138
                                               +hLa+rgl++++p+++r+G al+ L G+Pa++v+fL+G sv++P +++cr+ g  la lh ag df+ +
  lcl|NCBI__GCF_000019365.1:WP_012335681.1  70 EHLAARGLACPQPIRNRAGTALGRLCGRPAVIVSFLDGVSVRRPGVRHCRALGRALAGLHAAGVDFPMR 138
                                               ********************************************************************* PP

                                 TIGR00938 139 rkndlrleaWsilaakkfkvleqleeelaalldkeldalkkflprdLPrgvihadlfkdnvlldgdklk 207
                                               r n l+ +aW++l+a+    ++ + ++laa  +++l+ l++ +prdLP gvihadlf dnv++ gd ++
  lcl|NCBI__GCF_000019365.1:WP_012335681.1 139 RPNALSVAAWRPLFAQAEAQADRVAPNLAARTRDDLARLEEAWPRDLPGGVIHADLFTDNVFFIGDAVS 207
                                               *****************999************************************************* PP

                                 TIGR00938 208 gvidfyfaCedallydlaiavndWcfeaddkldaaaakallkgyeavrpLseeekaafpvllrgaalrf 276
                                               g+idfyfaC+da++ydlai++n+Wcfe d++++ ++ +a++ +y+a rpL + e + +p l+rgaalrf
  lcl|NCBI__GCF_000019365.1:WP_012335681.1 208 GLIDFYFACTDAFAYDLAICLNAWCFELDGSFNRTKGQAMIAAYQAARPLDPREVESLPLLARGAALRF 276
                                               ********************************************************************* PP

                                 TIGR00938 277 llsrlldlvftqagelvvakdPaeferkLk 306
                                               +l+rl+d+ + ++g+lv++kdP e+ rkL+
  lcl|NCBI__GCF_000019365.1:WP_012335681.1 277 MLTRLVDWLNVPPGALVKPKDPLEYDRKLA 306
                                               ****************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (307 nodes)
Target sequences:                          1  (321 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 6.75
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis