Alignments for a candidate for serB in Chlorobium limicola DSM 245

GapMind for Amino acid biosynthesis

Alignments for a candidate for serB in Chlorobium limicola DSM 245

Align Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 (characterized)
to candidate WP_012465196.1 CLIM_RS01150 phosphoserine phosphatase SerB

Query= SwissProt::Q9S281
         (410 letters)



>NCBI__GCF_000020465.1:WP_012465196.1
          Length = 405

 Score =  264 bits (675), Expect = 3e-75
 Identities = 164/402 (40%), Positives = 226/402 (56%), Gaps = 17/402 (4%)

Query: 12  LLVKIFGKDRPGITAGLFDTLAAYSVDVVDIEQVVTRGRIVLCALVTEPPRGLEGDLRAT 71
           LL+ I G D+PG+T+ +   LA   V V+DI Q V    + L  LV  P       +   
Sbjct: 5   LLINITGLDKPGLTSKIAAILADAKVPVLDIGQAVIHNHLSLGMLVEVPKESASSPVLKD 64

Query: 72  VHSWAESLKLQAEIISGIGDNRPRGFG-----RSLVTVLGHPLTAEATAAIAARITESGS 126
           +   A +L +Q        +   R  G     R L+++L   ++AE    +++ +     
Sbjct: 65  LLFCAHTLGIQISFTPVADEEYDRWVGEQGKPRYLLSLLSRKISAEQLKRVSSIVAAHHL 124

Query: 127 NIDRIFRLA---------KYPVTAVEFAVSGVETEP--LRTALATEAAALGVDIAVVAAG 175
           NID I RL+          +    VEF++ G  ++    R  L     +LG+DIA     
Sbjct: 125 NIDTISRLSGRIPLDNGDNHTRACVEFSLRGTLSDENLFREQLLAITDSLGIDIAFQEDN 184

Query: 176 LHRRAQRLVVMDVDSTLIQDEVIELFAAHAGCEDEVAEVTAAAMRGELDFEQSLHARVAL 235
           + RR +R+VV D+DSTLI  EVI+  A  AG  +EV+ +T  AMRGELDF +SL  RVA 
Sbjct: 185 IFRRNRRMVVFDMDSTLITSEVIDELAKEAGVGEEVSAITEQAMRGELDFNESLQRRVAQ 244

Query: 236 LAGLDASVVDKVRAEVRLTPGARTLIRTLKRLGYQVGVVSGGFTQVTDALQEQLGLDFAQ 295
           L GLD  V++ + A ++LT GA  L   LKRLGY+  ++SGGFT     LQ++L +D+  
Sbjct: 245 LRGLDEHVMESIAARLQLTEGAERLFGNLKRLGYKTAILSGGFTYFGHYLQKKLSIDYVY 304

Query: 296 ANTLEIVDGRLTGRVTGEIVDRAGKARLLRRFAAAAGVPLSQTVAIGDGANDLDMLNAAG 355
           ANTLEI +G LTGRV G +VD A KA LL   A    + L QTVA+GDGANDL ML  AG
Sbjct: 305 ANTLEIENGCLTGRVLGRVVDGARKAELLELLAEKENISLEQTVAVGDGANDLPMLGKAG 364

Query: 356 LGVAFNAKPVVREAAHTAVNVPFLDTVLYLLGITREEVEAAD 397
           LG+AF AKP+VRE A  A++   LD +LYL+G  R+  E A+
Sbjct: 365 LGIAFRAKPIVRERAKQAISTLGLDAILYLMGF-RDRDELAE 405


Lambda     K      H
   0.319    0.135    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 347
Number of extensions: 14
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 410
Length of database: 405
Length adjustment: 31
Effective length of query: 379
Effective length of database: 374
Effective search space:   141746
Effective search space used:   141746
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

Align candidate WP_012465196.1 CLIM_RS01150 (phosphoserine phosphatase SerB)
to HMM TIGR00338 (serB: phosphoserine phosphatase SerB (EC 3.1.3.3))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00338.hmm
# target sequence database:        /tmp/gapView.422.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00338  [M=219]
Accession:   TIGR00338
Description: serB: phosphoserine phosphatase SerB
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    7.7e-86  273.2   0.3      1e-85  272.8   0.3    1.2  1  lcl|NCBI__GCF_000020465.1:WP_012465196.1  CLIM_RS01150 phosphoserine phosp


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000020465.1:WP_012465196.1  CLIM_RS01150 phosphoserine phosphatase SerB
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  272.8   0.3     1e-85     1e-85       1     218 [.     177     394 ..     177     395 .. 0.99

  Alignments for each domain:
  == domain 1  score: 272.8 bits;  conditional E-value: 1e-85
                                 TIGR00338   1 diakselskllkkkklvvfDlDstlieeEvIdeiaklaGveeeVseiTerAmrgeldFkeslreRvkll 69 
                                               dia +e++ +++++++vvfD+Dstli++EvIde+ak+aGv+eeVs+iTe+AmrgeldF+esl+ Rv+ l
  lcl|NCBI__GCF_000020465.1:WP_012465196.1 177 DIAFQEDNIFRRNRRMVVFDMDSTLITSEVIDELAKEAGVGEEVSAITEQAMRGELDFNESLQRRVAQL 245
                                               68899999************************************************************* PP

                                 TIGR00338  70 kglpvellkkveeklelteGveelvkkLkekgykvaviSGgFdlvaeklkekLgldavfaNrLevedgk 138
                                               +gl++++++ ++ +l+lteG+e l  +Lk+ gyk+a++SGgF+++  +l++kL +d+v aN+Le+e+g 
  lcl|NCBI__GCF_000020465.1:WP_012465196.1 246 RGLDEHVMESIAARLQLTEGAERLFGNLKRLGYKTAILSGGFTYFGHYLQKKLSIDYVYANTLEIENGC 314
                                               ********************************************************************* PP

                                 TIGR00338 139 ltGkvegeivdesakaktllkllekegislektvavGDGanDlsmikaAglgiafnakpvlkekadivi 207
                                               ltG+v g++vd   ka+ l+ l+eke+isle+tvavGDGanDl+m+ +Aglgiaf akp+++e+a+++i
  lcl|NCBI__GCF_000020465.1:WP_012465196.1 315 LTGRVLGRVVDGARKAELLELLAEKENISLEQTVAVGDGANDLPMLGKAGLGIAFRAKPIVRERAKQAI 383
                                               ********************************************************************* PP

                                 TIGR00338 208 ekkdltdilel 218
                                               ++  l +il+l
  lcl|NCBI__GCF_000020465.1:WP_012465196.1 384 STLGLDAILYL 394
                                               *****999987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (219 nodes)
Target sequences:                          1  (405 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.00
# Mc/sec: 11.09
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis