Align homoserine kinase (EC 2.7.1.39) (characterized)
to candidate WP_012467221.1 CLIM_RS11715 homoserine kinase
Query= reanno::Cola:Echvi_2001 (311 letters) >NCBI__GCF_000020465.1:WP_012467221.1 Length = 320 Score = 297 bits (761), Expect = 2e-85 Identities = 160/320 (50%), Positives = 208/320 (65%), Gaps = 12/320 (3%) Query: 1 MKKVTAFAPATVANVSCGFDILGFAVEEMGDKVTVSLADEPG----VRVVRIEGDGGRLP 56 MK V FA ATV NV+CGFD+LGFA+ E GD+VT++L DE V + I GDGG LP Sbjct: 1 MKTVKGFASATVGNVACGFDVLGFAITEPGDEVTLTLLDERKKDCPVSISGITGDGGALP 60 Query: 57 YEVDKNTCGVALKAFLA------AIDYKGGLEVELYKGLPLGSGMGSSAASSVAALEAAN 110 + KNT + FL +D+ G + +EL K LPL SGMGSSAAS+ AAL AAN Sbjct: 61 LDPKKNTSSFVVLKFLEYIRTHKGVDFTGHISLELKKNLPLSSGMGSSAASAAAALVAAN 120 Query: 111 QLLGNPLDKKALLPFAMKAEGVACGAEHADNVAPSLLGGFVLIRSYHPLDVTKLHVPKGL 170 +LLG P K L+ FA++ E VACG+ HADN AP++LG FVLIRSY PLD+ + PK L Sbjct: 121 ELLGQPCTKMELVHFAIEGERVACGSAHADNAAPAILGNFVLIRSYTPLDLITIPPPKDL 180 Query: 171 YCALVHPHLELNTADSRSVLRQQVPLKDAITQSGNIAGLVAGLYQEDMGLISRSLQDVIA 230 +C LVHPH EL T+ +RSVL + +PLK AI Q GN+ L+ GL D LI RSL DV+A Sbjct: 181 FCTLVHPHTELRTSFARSVLPRAIPLKTAIQQWGNVGALITGLLTSDYELIGRSLVDVVA 240 Query: 231 EPSRSVLIPGFDEIKSAIKEVGALGVGISGSGPTTFILAPSREVAERASEICQEVF--DK 288 EP R+ LIPGF ++K A + GALG I+GSGP+ F + S + A A Q+ F K Sbjct: 241 EPKRAPLIPGFFDVKHAALDAGALGCSIAGSGPSLFAFSSSEKTAREAGNAMQQAFLSPK 300 Query: 289 IGLEVDLYVSAVNTKGTYVI 308 L+ D++VS + ++G ++ Sbjct: 301 TNLDSDMWVSRICSEGAKIL 320 Lambda K H 0.317 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 252 Number of extensions: 12 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 311 Length of database: 320 Length adjustment: 27 Effective length of query: 284 Effective length of database: 293 Effective search space: 83212 Effective search space used: 83212 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 48 (23.1 bits)
Align candidate WP_012467221.1 CLIM_RS11715 (homoserine kinase)
to HMM TIGR00191 (thrB: homoserine kinase (EC 2.7.1.39))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00191.hmm # target sequence database: /tmp/gapView.11440.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00191 [M=304] Accession: TIGR00191 Description: thrB: homoserine kinase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.6e-62 197.0 0.0 1.8e-62 196.9 0.0 1.0 1 lcl|NCBI__GCF_000020465.1:WP_012467221.1 CLIM_RS11715 homoserine kinase Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000020465.1:WP_012467221.1 CLIM_RS11715 homoserine kinase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 196.9 0.0 1.8e-62 1.8e-62 6 279 .. 9 294 .. 4 320 .] 0.86 Alignments for each domain: == domain 1 score: 196.9 bits; conditional E-value: 1.8e-62 TIGR00191 6 PassANlgpGfDvlGlalslv.lellvtedvaqeskdk...sleaegegvekipkesdkNliyqvakkv 70 a+ N++ GfDvlG a+++ +e+++t + +k + s+ + + +p++++kN v++k+ lcl|NCBI__GCF_000020465.1:WP_012467221.1 9 SATVGNVACGFDVLGFAITEPgDEVTLTL-LDERKK-DcpvSISGITGDGGALPLDPKKNTSSFVVLKF 75 58999*************98735566665.443344.346788888888899********888888888 PP TIGR00191 71 lkklgkr.....vkpvkltvekeiplgrGLGSSaaaivaaviaanelaglklskeelldlalllEg... 131 l+ + + l+++k++pl++G+GSSaa+ +aa++aanel+g++++k+el+++a++ E+ lcl|NCBI__GCF_000020465.1:WP_012467221.1 76 LEYIRTHkgvdfTGHISLELKKNLPLSSGMGSSAASAAAALVAANELLGQPCTKMELVHFAIEGERvac 144 776665544556677899*************************************************** PP TIGR00191 132 ...HpDNvapallGGlqlavkeddllevlkvPsgsklkvvlviPnievsTaeaRavLPkaysrqdlvfn 197 H+DN+apa+lG ++l ++ l + +P ++l + lv P+ e+ T aR vLP+a +++ ++++ lcl|NCBI__GCF_000020465.1:WP_012467221.1 145 gsaHADNAAPAILGNFVLIRSYTP-LDLITIPPPKDLFCTLVHPHTELRTSFARSVLPRAIPLKTAIQQ 212 *******************99999.888888888*********************************** PP TIGR00191 198 lshlavlvtAlvskdkadllaiamkDrvhqpyRekliPelteikqaakekgalgitlSGaGptilalae 266 +++ l+t l + +l+ + D+v++p+R+ liP+++++k+aa ++galg + G+Gp+++a+++ lcl|NCBI__GCF_000020465.1:WP_012467221.1 213 WGNVGALITGLLTS-DYELIGRSLVDVVAEPKRAPLIPGFFDVKHAALDAGALGCSIAGSGPSLFAFSS 280 **************.6677777788*******************************************9 PP TIGR00191 267 eek.eekaqellek 279 ek ++ a +++++ lcl|NCBI__GCF_000020465.1:WP_012467221.1 281 SEKtAREAGNAMQQ 294 99955555555544 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (304 nodes) Target sequences: 1 (320 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 11.91 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory