Alignments for a candidate for aspS2 in Geobacter lovleyi SZ

GapMind for Amino acid biosynthesis

Alignments for a candidate for aspS2 in Geobacter lovleyi SZ

Align aspartyl-tRNA(Asp/Asn) synthetase (EC 6.1.1.23) (characterized)
to candidate WP_012469680.1 GLOV_RS08050 aspartate--tRNA ligase

Query= reanno::Miya:8501305
         (614 letters)



>NCBI__GCF_000020385.1:WP_012469680.1
          Length = 598

 Score =  735 bits (1897), Expect = 0.0
 Identities = 352/589 (59%), Positives = 450/589 (76%), Gaps = 4/589 (0%)

Query: 16  IEPLGDWVRSHDCATLTAADTGAEVCLMGWVQYRRDHGGLIFVDLRDRQGLTQIVFSPDV 75
           ++ LG+W RS  C  LTA D G EV LMGW   RRDHGGLIF+DLRDR+G+ Q+VF P+V
Sbjct: 1   MDTLGNWKRSDYCGCLTAQDIGREVVLMGWALRRRDHGGLIFIDLRDREGIAQVVFDPEV 60

Query: 76  NPAAHERAHIIRSEYVLAIRGTVRPRPEGMTNPGMKTGEIEVYVSEWKLLNTSKTPAFVI 135
           N +AH+ A  +RSEYVLAI+G V PRPEG  NP MKTGE+E+ V+E KLLN SK   F++
Sbjct: 61  NASAHQVAEGVRSEYVLAIKGKVIPRPEGTVNPNMKTGEVEIQVAECKLLNRSKALPFML 120

Query: 136 EDRTEASENLRLQYRYLDLRRPRMANNFIVRHKAAQATRRYLDELGFLEIETPYLTKSTP 195
           ++ +E +EN+RL+YRYLD+RRP++A N I+RH+ AQ TR+YL + GFLE+ETP+LTKSTP
Sbjct: 121 DEHSEVNENIRLKYRYLDMRRPQLAQNLILRHQVAQVTRQYLTDNGFLELETPFLTKSTP 180

Query: 196 EGARDFLVPSRLNQGQFYALPQSPQLFKQLLMMSGMERYYQIVRCFRDEDMRADRQLEFT 255
           EGARDFLVPSR+NQGQFYALPQSPQ+FKQ+LM+SG +RY+QIVRCFRDED+RADRQ EFT
Sbjct: 181 EGARDFLVPSRINQGQFYALPQSPQIFKQILMISGFDRYFQIVRCFRDEDLRADRQPEFT 240

Query: 256 QIDIEMSFVDEEQVMAMAEGLMARVFRDALDREIATPFPRMSYDDAMARYGVDKPDTRFG 315
           QID EMSF+D E ++ + EGL+ARVF++A   E+  P  RM+Y +A+ R+GVD PD RFG
Sbjct: 241 QIDCEMSFIDREDIITVMEGLIARVFKEAKGVEVQLPVERMTYQEAIRRFGVDNPDLRFG 300

Query: 316 LELQDVTHIVRGSNFKLF----ATAELVKAMRVPGGETMTRKEIDEFTEFVKIYGAQGLA 371
           LEL ++T IV+GS FK+F    A   ++K +   G  T +RK+I++ T FV IYGA+GLA
Sbjct: 301 LELVELTDIVKGSGFKVFNEVAANGGIIKGINAKGCATFSRKDIEDLTSFVSIYGAKGLA 360

Query: 372 WIKIRPDEWQSPIAKFLSDEERAGLKDALGLETGDIVFFQAGAPGMVNAALGNLRVRLGE 431
           ++K+  + WQSPI KF ++ E A +  + G E GD++ F A  P +VN +LG LR +L  
Sbjct: 361 YVKVTAEGWQSPITKFFTEAEIAAMNGSFGAEEGDLLLFVADKPKVVNDSLGKLRNQLAS 420

Query: 432 HLGLIDENAFNFLWVTDFPLFEYDEEEKRYVACHHPFTSPKDGHMDVMVENPAAARARAY 491
            L L D N F F+W+TDFPLFE+DE++KR+ A HHPFT+P D  +D +  +P   RA+AY
Sbjct: 421 LLKLTDPNVFRFVWITDFPLFEWDEDDKRWCAVHHPFTAPMDEDVDYVESDPGRCRAKAY 480

Query: 492 DMVLNGYELGGGSIRNHTAEKQRRMFAALGFSPEEAEAQFGFLTQALEMGAPPHGGIAFG 551
           D+VLNG E+GGGSIR H    Q  MF  LG S E+  A+FGFL  ALE G PPHGGIAFG
Sbjct: 481 DLVLNGNEIGGGSIRIHQEHVQSLMFKMLGHSEEDKRAKFGFLLDALEYGTPPHGGIAFG 540

Query: 552 MDRLAMLLTGSSSIRDVIAFPKTQKATCLLTQAPDSVSARQLRDLGLRL 600
           MDRL MLLTGS SIRDVIAFPKTQK TCL+++AP +V A+QLR+LG+RL
Sbjct: 541 MDRLIMLLTGSDSIRDVIAFPKTQKGTCLMSEAPSAVDAKQLRELGVRL 589


Lambda     K      H
   0.321    0.137    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 979
Number of extensions: 28
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 614
Length of database: 598
Length adjustment: 37
Effective length of query: 577
Effective length of database: 561
Effective search space:   323697
Effective search space used:   323697
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis