Alignments for a candidate for metH in Geobacter lovleyi SZ

GapMind for Amino acid biosynthesis

Alignments for a candidate for metH in Geobacter lovleyi SZ

Align Methionine synthase; EC 2.1.1.13; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS (uncharacterized)
to candidate WP_012470188.1 GLOV_RS10575 methionine synthase

Query= curated2:O33259
         (1192 letters)



>NCBI__GCF_000020385.1:WP_012470188.1
          Length = 1169

 Score =  771 bits (1992), Expect = 0.0
 Identities = 459/1193 (38%), Positives = 691/1193 (57%), Gaps = 60/1193 (5%)

Query: 13   LLDVLSQRVMVGDGAMGTQLQAADLTLDDFRG--LEGCNEILNETRPDVLETIHRNYFEA 70
            +L  LSQ +++ DGAMGTQLQA +LT  DF G   EGCNE+L  TRPDV+E +H+ Y EA
Sbjct: 6    VLQALSQCILILDGAMGTQLQARNLTAADFGGEAYEGCNEMLVLTRPDVIEDVHKAYLEA 65

Query: 71   GADAVETNTFGCNLSNLGDYDIADRIRDLSQKGTAIARRVADELGSPDRKRYVLGSMGPG 130
            GAD VET +FG     L +Y +AD++ +L++    +AR+  D   +PD+ R+V GSMGP 
Sbjct: 66   GADIVETCSFGSTDIVLAEYGLADKVFELNKAAALVARKACDAYSTPDKPRFVAGSMGPT 125

Query: 131  TK-LPTLGHTEYAVIRDAYTEAALGMLDGGADAILVETCQDLLQLKAAVLGSRRAMTRAG 189
            T+ +   G   +  +  A+ +  +G+L GG D +L+ET QD L LKA   G R A  + G
Sbjct: 126  TRTISVTGGVTFEQLVTAFRDQTIGLLAGGVDLLLLETAQDTLNLKAGAEGIRLAFEQTG 185

Query: 190  RHIPVFAHVTVETTGTMLLGSEIGAALTAV----EPLGVDMIGLNCATGPAEMSEHLRHL 245
            + +P+    T+E TGTML G  + A   ++    E LG+  IGLNCATGP  M++HLR L
Sbjct: 186  QRVPLMVSGTIEPTGTMLAGQNVEALYASLTHLEENLGLISIGLNCATGPEFMTDHLRAL 245

Query: 246  SRHARIPVSVMPNAGLPVLGAKGAEYPLLPDELAEALAGFIAEFGLSLVGGCCGTTPAHI 305
            S  A   +SV PNAGLP    +   Y   PD LA+ L+ F+ E  L+++GGCCGT+PAHI
Sbjct: 246  SELATCHISVYPNAGLP---DENGNYAESPDSLAQKLSRFVDEGWLNIIGGCCGTSPAHI 302

Query: 306  REVAAAVANIKRPERQVSYEPSVSSLYTAIPFAQDASVLVIGERTNANGSKGFREAMIAE 365
              +A   A  ++P + V+    + S    +   +D   +++GERTN  GS+ F+  ++A 
Sbjct: 303  AAIANMAAG-RQPRKPVTIRRRLVSGIEPLFIEEDNRPILVGERTNVIGSRKFKNMIVAG 361

Query: 366  DYQKCLDIAKDQTRDGAHLLDLCVDYVGRDGVADMKALASRLATSSTLPIMLDSTETAVL 425
             +++  +IA+ Q + GA ++D+CV    RD +ADM+ +   L      PIM+DST+  V 
Sbjct: 362  QFEEAAEIARAQVKTGAQVIDICVANPDRDELADMEQMLVYLPKKVRAPIMIDSTDAKVT 421

Query: 426  QAGLEHLGGRCAINSVNYEDGDGPESRFAKTMALVAEHGAAVVALTIDEE---GQARTAQ 482
            +  L+ L G+C INS+N EDG   E RFAK   L+  +G AVV   IDE+   G A T Q
Sbjct: 422  ELALQRLQGKCVINSINLEDG---EERFAKVAPLLRHYGGAVVVGCIDEDPANGMAVTRQ 478

Query: 483  KKVEIAERLINDITGNWGVDESSILIDTLTFTIATGQEESRRDGIETIEAIRELKKRHPD 542
            +K+E+A+R  + +   +G+    ++ D L F + +G +      +ETIE +R + +  P 
Sbjct: 479  RKLEVAQRSYDLLVNKYGLRPEDLIFDPLVFPVGSGDDNYIGSAVETIEGVRLITETFPQ 538

Query: 543  VQTTLGLSNISFGLNPAARQVLNSVFLHECQEAGLDSAIVHASKILPMNRIPEEQRNVAL 602
              T LG+SN+SFGL  A R+VLN+VFL+ C +AGL  AIV+  K+     IPE +R +A 
Sbjct: 539  CSTILGISNVSFGLPAAGREVLNAVFLYHCVKAGLGYAIVNTEKLERYASIPEAERRLAE 598

Query: 603  DLVYDRRREDYDPLQELMRLFEGVSAASSKEDRLAELAGLPLFERLAQRIVDGERNGLDA 662
            DL++ R     DP+      F      S      A  A LPL ERL   I++G ++GL A
Sbjct: 599  DLIFWR---GADPVAAFAAAFRDKKPVSH-----APAAELPLDERLPLYIIEGSKDGLTA 650

Query: 663  DLDEAMTQ-KPPLQIINEHLLAGMKTVGELFGSGQMQLPFVLQSAEVMKAAVAYLEPHME 721
            DLD A+T+   PL+IIN  L+AGM  VG LF   Q+ +  VLQSAE MKAAV++LEPH+ 
Sbjct: 651  DLDAALTRGDKPLEIINGPLMAGMAEVGRLFNDNQLIVAEVLQSAEAMKAAVSHLEPHLS 710

Query: 722  RSDDDSGKGRIVLATVKGDVHDIGKNLVDIILSNNGYEVVNIGIKQPIATILEVAEDKSA 781
            + D+ S KG+++LATVKGDVHDIGKNLV+IILSNNG+EV+N+GIK     ++  A+ ++ 
Sbjct: 711  K-DETSSKGKMLLATVKGDVHDIGKNLVEIILSNNGFEVINLGIKVGPEELIAAAKKENP 769

Query: 782  DVVGMSGLLVKSTVVMKENLEEMNTRGVAEKFPVLLGGAALTRSYVENDLAEIYQGEVHY 841
            D +G+SGLLVKS + M     ++   G+    P+L+GGAAL+R++ +  +   Y G V Y
Sbjct: 770  DFIGLSGLLVKSALQMIVTAADLKAAGI--DAPLLVGGAALSRAFADTRITPEYNGPVLY 827

Query: 842  ARDAFEGLKLMDTIMS-AKRGEAPDENSPEAIKAREKEAERKARHQRSKRIAAQRKAAEE 900
            A+DA  GL+L + ++  A R +   +       AR++EA        S +IAA + A + 
Sbjct: 828  AKDAMAGLELANQLVDPALRQQLMLD------LARQQEA--------SAKIAAAKAAGQS 873

Query: 901  PVEV-PERSDVAADIEVPAPPFWGSRIVKGLAVADYTGLLDERALFLGQWGLRGQRGGEG 959
             V      S ++++  +   P     I++ + V      L+ + L+    GL G    + 
Sbjct: 874  TVATGSTHSAISSNAPILVAPDLEQHILRDIPVGQIIPYLNRQMLYTKHLGLTGS-VDKL 932

Query: 960  PSYEDLVETEGRPRLRYWLDRLSTDGILAHAAVVYGYFPAVSEGNDIVVLTEPKPDAPVR 1019
             + +D   T+    +   L+R+  +G++   A +Y ++ A  +GND+++  +   +A   
Sbjct: 933  LAGQDEKATKLHLTVEAMLERVLQEGLIKPQA-IYRFYQANGDGNDLILFNQDGSEAT-- 989

Query: 1020 YRFHFPRQQRGRFLCIADFIRSRELAAERGEVDVLPFQLVTMGQPIADFANELFASNAYR 1079
             RF  PRQ+ G  LC+ADF+R         E D +    VT GQ + + + +  A   Y 
Sbjct: 990  -RFTLPRQKSGEQLCVADFVR----PLSGTEKDTMALFAVTCGQGVRELSEQWKAEGDYL 1044

Query: 1080 DYLEVHGIGVQLTEALAEYWHRRIREELKFSGDRAMAAEDPEAKEDYFKLGYRGARFAFG 1139
            +   +  + +++ EA AEY H+RIR       D+ +        +  F   Y+G R +FG
Sbjct: 1045 NSHLLQALALEMAEATAEYLHKRIRTSWGIVDDQTL------TMKQLFNAEYQGIRVSFG 1098

Query: 1140 YGACPDLEDRAKMMALLEPERIGVTLSEELQLHPEQSTDAFVLHHPEAKYFNV 1192
            Y ACP+L+D+ K+ +LL+PE+IG+ L+E   + PE S  A V HHPE KYF++
Sbjct: 1099 YPACPNLDDQKKLFSLLKPEQIGINLTEGDMMDPEASVSALVFHHPEGKYFDL 1151


Lambda     K      H
   0.317    0.135    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 2978
Number of extensions: 121
Number of successful extensions: 17
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 1192
Length of database: 1169
Length adjustment: 47
Effective length of query: 1145
Effective length of database: 1122
Effective search space:  1284690
Effective search space used:  1284690
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)

Align candidate WP_012470188.1 GLOV_RS10575 (methionine synthase)
to HMM TIGR02082 (metH: methionine synthase (EC 2.1.1.13))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR02082.hmm
# target sequence database:        /tmp/gapView.5930.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR02082  [M=1182]
Accession:   TIGR02082
Description: metH: methionine synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
          0 1227.0   0.0          0 1226.8   0.0    1.0  1  lcl|NCBI__GCF_000020385.1:WP_012470188.1  GLOV_RS10575 methionine synthase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000020385.1:WP_012470188.1  GLOV_RS10575 methionine synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 ! 1226.8   0.0         0         0       2    1181 ..      11    1150 ..      10    1151 .. 0.95

  Alignments for each domain:
  == domain 1  score: 1226.8 bits;  conditional E-value: 0
                                 TIGR02082    2 nkrilvlDGamGtqlqsanLteadFrgeeadlarelkGnndlLnltkPeviaaihrayfeaGaDive 68  
                                                ++ il+lDGamGtqlq+ nLt+adF ge       ++G+n++L+lt+P+vi+++h+ay+eaGaDive
  lcl|NCBI__GCF_000020385.1:WP_012470188.1   11 SQCILILDGAMGTQLQARNLTAADFGGE------AYEGCNEMLVLTRPDVIEDVHKAYLEAGADIVE 71  
                                                788************************7......9******************************** PP

                                 TIGR02082   69 tntFnsteialadYdledkayelnkkaaklarevadeftltpekkRfvaGslGPtnklatlspdver 135 
                                                t  F+st i+la+Y+l+dk+ elnk+aa +ar+++d ++ tp+k+RfvaGs+GPt+++ +++     
  lcl|NCBI__GCF_000020385.1:WP_012470188.1   72 TCSFGSTDIVLAEYGLADKVFELNKAAALVARKACDAYS-TPDKPRFVAGSMGPTTRTISVT----- 132 
                                                ***************************************.*********************9..... PP

                                 TIGR02082  136 pefrnvtydelvdaYkeqvkglldGGvDllLietvfDtlnakaalfaveevfeekgrelPilisgvi 202 
                                                     vt+++lv a+++q+ gll GGvDllL+et +Dtln+ka   +++ +fe+ g+++P+++sg+i
  lcl|NCBI__GCF_000020385.1:WP_012470188.1  133 ---GGVTFEQLVTAFRDQTIGLLAGGVDLLLLETAQDTLNLKAGAEGIRLAFEQTGQRVPLMVSGTI 196 
                                                ...789**********************************************************876 PP

                                 TIGR02082  203 vdksGrtLsGqtleaflaslehae....ililGLnCalGadelrefvkelsetaealvsviPnaGLP 265 
                                                 + +G++L Gq++ea +asl h e    ++++GLnCa+G++ + +++++lse a++ +sv+PnaGLP
  lcl|NCBI__GCF_000020385.1:WP_012470188.1  197 -EPTGTMLAGQNVEALYASLTHLEenlgLISIGLNCATGPEFMTDHLRALSELATCHISVYPNAGLP 262 
                                                .899***************987542222899************************************ PP

                                 TIGR02082  266 nalgeYdltpeelakalkefaeegllnivGGCCGttPehiraiaeavkdikprkrqeleeksvlsgl 332 
                                                ++ g+Y + p++la++l  f+ eg lni+GGCCGt P+hi+aia+++++ +prk  ++    ++sg+
  lcl|NCBI__GCF_000020385.1:WP_012470188.1  263 DENGNYAESPDSLAQKLSRFVDEGWLNIIGGCCGTSPAHIAAIANMAAGRQPRKPVTIRR-RLVSGI 328 
                                                *****************************************************9887765.5689** PP

                                 TIGR02082  333 ealkiaqessfvniGeRtnvaGskkfrklikaedyeealkiakqqveeGaqilDinvDevllDgead 399 
                                                e+l i++++  + +GeRtnv Gs+kf+++i a+++eea +ia+ qv+ Gaq++Di+v +  +D  ad
  lcl|NCBI__GCF_000020385.1:WP_012470188.1  329 EPLFIEEDNRPILVGERTNVIGSRKFKNMIVAGQFEEAAEIARAQVKTGAQVIDICVANPDRDELAD 395 
                                                ******************************************************************* PP

                                 TIGR02082  400 mkkllsllasepdiakvPlmlDssefevleaGLkviqGkaivnsislkdGeerFlekaklikeyGaa 466 
                                                m+++l  l ++  + + P+m+Ds++ +v e  L+++qGk+++nsi+l+dGeerF++ a l+++yG a
  lcl|NCBI__GCF_000020385.1:WP_012470188.1  396 MEQMLVYLPKK--V-RAPIMIDSTDAKVTELALQRLQGKCVINSINLEDGEERFAKVAPLLRHYGGA 459 
                                                ****9999888..5.9*************************************************** PP

                                 TIGR02082  467 vvvmafDee...GqartadkkieiakRayklltekvgfppediifDpniltiatGieehdryaidfi 530 
                                                vvv  +De+   G+a t+++k+e+a+R y+ll++k+g+ ped+ifDp++++++ G ++    a+++i
  lcl|NCBI__GCF_000020385.1:WP_012470188.1  460 VVVGCIDEDpanGMAVTRQRKLEVAQRSYDLLVNKYGLRPEDLIFDPLVFPVGSGDDNYIGSAVETI 526 
                                                ********6666******************************************************* PP

                                 TIGR02082  531 eaireikeelPdakisgGvsnvsFslrgndavRealhsvFLyeaikaGlDmgivnagklavyddidk 597 
                                                e +r i e++P++ + +G+snvsF+l    a Re+l++vFLy+++kaGl  +ivn+ kl+ y+ i++
  lcl|NCBI__GCF_000020385.1:WP_012470188.1  527 EGVRLITETFPQCSTILGISNVSFGLP--AAGREVLNAVFLYHCVKAGLGYAIVNTEKLERYASIPE 591 
                                                ***************************..999*********************************** PP

                                 TIGR02082  598 elrevvedlildrrreatekLlelaelykgtkeksskeaqeaewrnlpveeRLeralvkGeregiee 664 
                                                  r ++edli+ r     +++  +a  ++++k        +a  ++lp++eRL   +++G ++g+ +
  lcl|NCBI__GCF_000020385.1:WP_012470188.1  592 AERRLAEDLIFWR---GADPVAAFAAAFRDKKPV-----SHAPAAELPLDERLPLYIIEGSKDGLTA 650 
                                                ***********98...57899********99887.....457899********************** PP

                                 TIGR02082  665 dleearkklkapleiiegpLldGmkvvGdLFGsGkmfLPqvvksarvmkkavayLePylekekeedk 731 
                                                dl++a+ +  +pleii+gpL++Gm  vG LF + ++ +  v++sa+ mk+av++LeP+l k++  ++
  lcl|NCBI__GCF_000020385.1:WP_012470188.1  651 DLDAALTRGDKPLEIINGPLMAGMAEVGRLFNDNQLIVAEVLQSAEAMKAAVSHLEPHLSKDE--TS 715 
                                                ***********************************************************9887..99 PP

                                 TIGR02082  732 skGkivlatvkGDvhDiGknivdvvLscngyevvdlGvkvPvekileaakkkkaDviglsGLivksl 798 
                                                skGk++latvkGDvhDiGkn+v+++Ls+ng+ev++lG+kv  e+++ aakk+++D iglsGL+vks 
  lcl|NCBI__GCF_000020385.1:WP_012470188.1  716 SKGKMLLATVKGDVHDIGKNLVEIILSNNGFEVINLGIKVGPEELIAAAKKENPDFIGLSGLLVKSA 782 
                                                ******************************************************************* PP

                                 TIGR02082  799 demvevaeemerrgvkiPlllGGaalskahvavkiaekYkgevvyvkdaseavkvvdkllsekkkae 865 
                                                 +m+  a +++  g++ Pll+GGaals+a+ ++ i ++Y+g+v+y+kda+++++++++l++   +++
  lcl|NCBI__GCF_000020385.1:WP_012470188.1  783 LQMIVTAADLKAAGIDAPLLVGGAALSRAFADTRITPEYNGPVLYAKDAMAGLELANQLVDPALRQQ 849 
                                                *************************************************************998877 PP

                                 TIGR02082  866 elekikeeyee.irekfgekkeklialsekaarkevfaldrsedlevpapkflGtkvleas.ieell 930 
                                                 +  + +++e  ++  + +    + ++        + +   s+++ + ++++l +++l+++ + +++
  lcl|NCBI__GCF_000020385.1:WP_012470188.1  850 LMLDLARQQEAsAKIAAAK-AAGQSTV-----ATGSTHSAISSNAPILVAPDLEQHILRDIpVGQII 910 
                                                7655544433303333322.2222222.....2233344555578899999**************** PP

                                 TIGR02082  931 kyiDwkalFv.qWelrgkypkilkdeleglearklfkdakelldklsaekllrargvvGlfPaqsvg 996 
                                                +y++ ++l++ + +l g+ +k l    ++++a+kl+ +++++l+++ +e+l+++++++ ++ a+  g
  lcl|NCBI__GCF_000020385.1:WP_012470188.1  911 PYLNRQMLYTkHLGLTGSVDKLLAG--QDEKATKLHLTVEAMLERVLQEGLIKPQAIYRFYQANGDG 975 
                                                **********899*******88875..67899*********************************** PP

                                 TIGR02082  997 ddieiytdetvsqetkpiatvrekleqlrqqsdrylclaDfiaskesGikDylgallvtaglgaeel 1063
                                                +d+ ++++++        a   +++  +rq+s+++lc+aDf+++ +  +kD +++++vt g+g++el
  lcl|NCBI__GCF_000020385.1:WP_012470188.1  976 NDLILFNQDGS------EA---TRFTLPRQKSGEQLCVADFVRPLSGTEKDTMALFAVTCGQGVREL 1033
                                                ******96665......33...35899*****************9999******************* PP

                                 TIGR02082 1064 akkleakeddydsilvkaladrlaealaellhervRkelwgyaeeenldkedllkerYrGirpafGY 1130
                                                +++++a+ d+ +s l++ala  +aea ae+lh+r+R   wg  +++ l +++l+  +Y+Gir +fGY
  lcl|NCBI__GCF_000020385.1:WP_012470188.1 1034 SEQWKAEGDYLNSHLLQALALEMAEATAEYLHKRIRTS-WGIVDDQTLTMKQLFNAEYQGIRVSFGY 1099
                                                ************************************87.666699********************** PP

                                 TIGR02082 1131 pacPdhtekatlleLleaeriGlklteslalaPeasvsglyfahpeakYfa 1181
                                                pacP+++++++l++Ll++e+iG++lte  ++ Peasvs+l+f+hpe kYf 
  lcl|NCBI__GCF_000020385.1:WP_012470188.1 1100 PACPNLDDQKKLFSLLKPEQIGINLTEGDMMDPEASVSALVFHHPEGKYFD 1150
                                                **************************************************6 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (1182 nodes)
Target sequences:                          1  (1169 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.07u 0.03s 00:00:00.10 Elapsed: 00:00:00.10
# Mc/sec: 13.57
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis