Align Shikimate kinase; SK; EC 2.7.1.71 (uncharacterized)
to candidate WP_012471128.1 GLOV_RS15295 shikimate kinase
Query= curated2:A1TKW2 (183 letters) >NCBI__GCF_000020385.1:WP_012471128.1 Length = 177 Score = 94.0 bits (232), Expect = 1e-24 Identities = 61/156 (39%), Positives = 86/156 (55%), Gaps = 5/156 (3%) Query: 1 MRGPQLSIHLIGLPGSGKSTVGRHLARRLGLPFIDSDHVIEQRIGGSIRGFFDREGEAAF 60 M P +I LIG+PG+GKST+G LA+ L F+D+D +I+ + S++ DR+G A Sbjct: 1 MPRPPSNIVLIGMPGAGKSTLGVILAKLAALQFVDTDILIQTALQRSLQEIVDRDGYLAL 60 Query: 61 RDLEEEVILELTEANVPPQVLATGGGVVIRPGNRTRLRERGTVVYLNASPEEIFRHIRHD 120 R EE V+LELT N V+ATGG V TRL+ G VV+L+ + R I + Sbjct: 61 RQAEERVLLELTVHNA---VIATGGSAVYSRRAMTRLKSDGLVVFLDLPLAVLERRIHNY 117 Query: 121 QTRPLLQVGSPLDRLRELQRERDPLYREAAHFVISP 156 TR L + P ++ +ER PLY A I+P Sbjct: 118 CTRGLAK--PPEQSFADMFQERRPLYSRYADITITP 151 Lambda K H 0.321 0.141 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 100 Number of extensions: 8 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 183 Length of database: 177 Length adjustment: 19 Effective length of query: 164 Effective length of database: 158 Effective search space: 25912 Effective search space used: 25912 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 44 (21.6 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory