Align glutamate-5-semialdehyde dehydrogenase (EC 1.2.1.41) (characterized)
to candidate WP_012474300.1 CPHAMN1_RS04305 gamma-glutamyl-phosphate reductase
Query= BRENDA::A7Y114 (420 letters) >NCBI__GCF_000020545.1:WP_012474300.1 Length = 417 Score = 314 bits (805), Expect = 3e-90 Identities = 178/417 (42%), Positives = 252/417 (60%), Gaps = 17/417 (4%) Query: 13 QAIEAKKASKSLMILSDKEKNDALHHIADVLERDFEAILAANEKDLRNGREQGFTEAFMD 72 Q + ++AS+ L LSD + N L+ +A+ + + + IL AN+KDL + D Sbjct: 8 QLEKVREASRKLCTLSDSDINSLLNDLANRIPGNSQTILEANQKDLDR---MDPNDPKYD 64 Query: 73 RLSLSQERIRDFAQGLRDVAELEDPTGKKLSDWTLENGLQVEKVTVPLGVIGMIYEARPN 132 RL LS R+ A +R+VAEL P G+ L TL NGL ++K TVPLGV+G+IYE+RPN Sbjct: 65 RLLLSTSRLESIASDIRNVAELPTPVGRILEKRTLPNGLALKKTTVPLGVVGIIYESRPN 124 Query: 133 VTVDATGLALKSGNAIVLKGGSSAINSNQAIVEVIHKALAETKIPQESVQFIASTDRAAT 192 VT D L LKSGNA VLKGGS A+ SN AIV++I + L + +++ + S DR A Sbjct: 125 VTFDVFALCLKSGNATVLKGGSDAMYSNMAIVDLIREVLVRNNVNPDTLYLLPS-DREAA 183 Query: 193 NQLFTMKEHVDVLIPRGGGKLIQAVVENATVPVLETGVGNCHIYIDKDADVEKAISILVN 252 + ++DV+IPRG +LI ++++VPV+ETG G H Y D D++ I+ N Sbjct: 184 GIMLNAVGYIDVIIPRGSQQLIDFARKSSSVPVIETGAGIVHTYFDLSGDLQMGKEIVFN 243 Query: 253 AKTDRPAVCNAAETLIVHKDWLAQHSDELISA---LKKENIHVHGDEHVLTIIPDAVP-- 307 AKT RP+VCNA +TL++H++ L D+L S L ++ + + D+ + P Sbjct: 244 AKTRRPSVCNALDTLLIHRERL----DDLASIAEPLAEKKVIIFADKDAYPALLSRYPSE 299 Query: 308 ----AGEDDWKNEYLSTDIAVKAVDDLVDAIAHIETYGTKHSEAIVTENQEAADKFLAIV 363 A E + E+LS ++VK VD L DA+ HI + + HSEA++ + D+FL V Sbjct: 300 LLQKAEEKHFGTEFLSLKMSVKTVDSLEDALKHIARFSSMHSEAVIASDAVVKDEFLKRV 359 Query: 364 DAAAIYHNASTRFTDGGALGFGAEIGISTQKLHARGPMGLPALTTVKFLMKGTGQIR 420 DAA +Y N ST FTDG G GAEIGISTQKLHARGPM L LT+ K++++G GQ R Sbjct: 360 DAAVVYANTSTAFTDGAQFGLGAEIGISTQKLHARGPMALQELTSYKWVIEGDGQTR 416 Lambda K H 0.315 0.131 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 422 Number of extensions: 22 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 420 Length of database: 417 Length adjustment: 32 Effective length of query: 388 Effective length of database: 385 Effective search space: 149380 Effective search space used: 149380 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 50 (23.9 bits)
Align candidate WP_012474300.1 CPHAMN1_RS04305 (gamma-glutamyl-phosphate reductase)
to HMM TIGR00407 (proA: glutamate-5-semialdehyde dehydrogenase (EC 1.2.1.41))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00407.hmm # target sequence database: /tmp/gapView.9710.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00407 [M=398] Accession: TIGR00407 Description: proA: glutamate-5-semialdehyde dehydrogenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.4e-127 411.8 0.0 1.6e-127 411.6 0.0 1.0 1 lcl|NCBI__GCF_000020545.1:WP_012474300.1 CPHAMN1_RS04305 gamma-glutamyl-p Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000020545.1:WP_012474300.1 CPHAMN1_RS04305 gamma-glutamyl-phosphate reductase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 411.6 0.0 1.6e-127 1.6e-127 2 398 .] 13 406 .. 12 406 .. 0.95 Alignments for each domain: == domain 1 score: 411.6 bits; conditional E-value: 1.6e-127 TIGR00407 2 keaalklaqlstaeknealskiadeLkaeaelilaanakdiaaakenGladalldrLlLteeklksiad 70 +ea+ kl +ls + n++l+ +a+ + ++++il+an+kd+ d+ +drLlL+ ++l+sia lcl|NCBI__GCF_000020545.1:WP_012474300.1 13 REASRKLCTLSDSDINSLLNDLANRIPGNSQTILEANQKDLDRMDP---NDPKYDRLLLSTSRLESIAS 78 89***************************************87666...5999**************** PP TIGR00407 71 dvkdvieLadPvGkvieareldeGLklervrvPlGvlgviyearPevivdvasLclktGnaviLkGgke 139 d+++v+eL+ PvG+++e+r+l +GL l++ +vPlGv+g+iye+rP+v+ dv +Lclk+Gna +LkGg++ lcl|NCBI__GCF_000020545.1:WP_012474300.1 79 DIRNVAELPTPVGRILEKRTLPNGLALKKTTVPLGVVGIIYESRPNVTFDVFALCLKSGNATVLKGGSD 147 ********************************************************************* PP TIGR00407 140 avrsnkalveviqdaleqtglpveavqliedpdreevkellkldeyvdlliPrGgnelvklikeestiP 208 a+ sn a+v++i++ l + + ++ l+ dre + +l+ y+d++iPrG+++l++ +++s +P lcl|NCBI__GCF_000020545.1:WP_012474300.1 148 AMYSNMAIVDLIREVLVRNNVNPDTLYLLPS-DREAAGIMLNAVGYIDVIIPRGSQQLIDFARKSSSVP 215 *****************************99.************************************* PP TIGR00407 209 vlehadGvChiyldesadlakakkvivdaktqrPstCnaietLLvnkaiaeefleeLekqleekgvelr 277 v+e+++G+ h+y d s dl++ k+++ +akt+rPs+Cna++tLL++++ + l ++++ l+ek v + lcl|NCBI__GCF_000020545.1:WP_012474300.1 216 VIETGAGIVHTYFDLSGDLQMGKEIVFNAKTRRPSVCNALDTLLIHRERLD-DLASIAEPLAEKKVIIF 283 ***********************************************9995.578999*********** PP TIGR00407 278 adalv.lkllelekateaev.skedfdkeflsldLsvkivedleeaiehirqygtkhsdailtedkkna 344 ad+ + +ll+ + + ++ +++f +eflsl++svk+v++le+a++hi ++++ hs+a++++d lcl|NCBI__GCF_000020545.1:WP_012474300.1 284 ADKDAyPALLSRYPSELLQKaEEKHFGTEFLSLKMSVKTVDSLEDALKHIARFSSMHSEAVIASDAVVK 352 9765514556555555544404558******************************************** PP TIGR00407 345 ekfvkevdsaavyvnastrfadGfrfGfGaevgistqklharGPvGLeaLvsyk 398 ++f+k+vd+a vy n+st f+dG +fG+Gae+gistqklharGP+ L+ L+syk lcl|NCBI__GCF_000020545.1:WP_012474300.1 353 DEFLKRVDAAVVYANTSTAFTDGAQFGLGAEIGISTQKLHARGPMALQELTSYK 406 *****************************************************8 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (398 nodes) Target sequences: 1 (417 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01 # Mc/sec: 8.39 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory