Align argininosuccinate lyase (EC 4.3.2.1) (characterized)
to candidate WP_012474743.1 CPHAMN1_RS06570 argininosuccinate lyase
Query= BRENDA::Q8DVX5 (460 letters) >NCBI__GCF_000020545.1:WP_012474743.1 Length = 466 Score = 441 bits (1134), Expect = e-128 Identities = 224/459 (48%), Positives = 309/459 (67%) Query: 2 TTKNHKLWGGRFEAGLAQWVEEFGASISFDQKLAEFDLKGSIAHVTMLGEKGIISQEDAA 61 +T LW RF + F +S+ D+ L + D+KGSIAHVTML E+ II E+A Sbjct: 6 STGKELLWQSRFSEPFDREALLFSSSVDVDKVLYQEDIKGSIAHVTMLSEEAIIPVEEAR 65 Query: 62 TIKAGLEDLLEEYKAGQLKFDVSNEDIHMNMESLLTAKIGPVAGKLHTARSRNDQVATDM 121 I GLED+ +E +G L D +EDIH +E+ L KIGP+AGK+H+ RSRNDQVATD Sbjct: 66 LIIEGLEDIEQEISSGALIPDWEDEDIHTVIENRLKEKIGPIAGKMHSGRSRNDQVATDT 125 Query: 122 HLYLKAKLDEVIEKLANLRTVLVDLADKHVHTIMPGYTHLQHAQPISFGHHLMAYYNMFT 181 LYLK + E+ E L L+T+LV+ AD + +TI+ GYTHLQ AQP+S GH+ +AY+NMF Sbjct: 126 RLYLKKVIGELGEALDGLKTLLVEKADIYRNTIIFGYTHLQRAQPVSAGHYYLAYFNMFL 185 Query: 182 RDSERFIFNVKHTDLSPLGAAALAGTTFPIDREMTAQLMGFAEPYSNSLDAVSDRDFILE 241 RD ER K D+SPLGAAA AG+T PI+ + + +L+ F++ + NS DAVSDRD I+E Sbjct: 186 RDGERLKDLFKRVDISPLGAAAFAGSTLPINAKRSMELLDFSDLFQNSTDAVSDRDIIIE 245 Query: 242 FLSNASILMMHMSRMCEEVISWCSHEYQFVTLSDTFSTGSSIMPQKKNPDMAELIRGKSG 301 F+S SI+MMH+SR CE++I W S E+ ++ +SD FSTGSSIMPQKKN D+AEL+RGK+G Sbjct: 246 FISACSIIMMHLSRFCEDLILWSSSEFNYIEISDAFSTGSSIMPQKKNADIAELVRGKTG 305 Query: 302 RVYANLFGLLTVMKALPLAYNKDLQEDKEGMFDTAETITVALDILAGMLSSMIVNDKHMA 361 RVY NL +LT+MK+LPL+YN+D+QEDK +FD+A + ++ I ML+ +N+K + Sbjct: 306 RVYGNLMSILTIMKSLPLSYNRDMQEDKPPLFDSAGSTVSSVRIFTKMLAHTRLNEKRLK 365 Query: 362 ESTQKDFSNATELADYLASKGMPFRQAHEIVGKLILECSKNGHYLQDVPLERYQTISDLI 421 T +D S ATE+A+YL K +PFR+AH I GK++ + L + LE Y+ DL Sbjct: 366 TLTGEDLSLATEIAEYLVRKNLPFREAHRITGKIVSHAIEKRTSLPKISLEEYRGFCDLF 425 Query: 422 EEDVYETLKSHTAVERRHSLGGTGFEQVKWQIAEAKKAL 460 ED+Y+ L ++ +HS G T F V+ QI A+ L Sbjct: 426 NEDLYDALTPEASINSKHSHGSTSFASVEQQITIARGKL 464 Lambda K H 0.318 0.132 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 576 Number of extensions: 17 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 460 Length of database: 466 Length adjustment: 33 Effective length of query: 427 Effective length of database: 433 Effective search space: 184891 Effective search space used: 184891 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate WP_012474743.1 CPHAMN1_RS06570 (argininosuccinate lyase)
to HMM TIGR00838 (argH: argininosuccinate lyase (EC 4.3.2.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00838.hmm # target sequence database: /tmp/gapView.7316.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00838 [M=455] Accession: TIGR00838 Description: argH: argininosuccinate lyase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5.4e-179 581.9 0.0 6.1e-179 581.7 0.0 1.0 1 lcl|NCBI__GCF_000020545.1:WP_012474743.1 CPHAMN1_RS06570 argininosuccinat Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000020545.1:WP_012474743.1 CPHAMN1_RS06570 argininosuccinate lyase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 581.7 0.0 6.1e-179 6.1e-179 1 454 [. 12 464 .. 12 465 .. 0.99 Alignments for each domain: == domain 1 score: 581.7 bits; conditional E-value: 6.1e-179 TIGR00838 1 lwggRlkkeldkavaefnaslsfDkelaeaDiegsiahtkaLakagilteeeakklieaLeelkeevke 69 lw++R+++ d ++ f++s++ Dk l+++Di+gsiah+++L ++ i+ eea+ +ie+Le++++e+++ lcl|NCBI__GCF_000020545.1:WP_012474743.1 12 LWQSRFSEPFDREALLFSSSVDVDKVLYQEDIKGSIAHVTMLSEEAIIPVEEARLIIEGLEDIEQEISS 80 7******************************************************************** PP TIGR00838 70 gklelevdaeDiHlavErelidkvgedvgkklhtgrsRnDqvatdlrlylrdkvkelaealkdllkalv 138 g+l +++++eDiH+++E++l +k+g +++k+h+grsRnDqvatd rlyl++ + el eal l++ lv lcl|NCBI__GCF_000020545.1:WP_012474743.1 81 GALIPDWEDEDIHTVIENRLKEKIG-PIAGKMHSGRSRNDQVATDTRLYLKKVIGELGEALDGLKTLLV 148 *************************.9999*************************************** PP TIGR00838 139 ekAekevetlmpgytHLqrAqPitlaHhllayaemlerDleRlldalkRvnksPlGsgAlagtsfeidr 207 ekA+ +t++ gytHLqrAqP++ +H+ lay++m+ rD eRl+d +kRv+ sPlG++A+ag++++i+ lcl|NCBI__GCF_000020545.1:WP_012474743.1 149 EKADIYRNTIIFGYTHLQRAQPVSAGHYYLAYFNMFLRDGERLKDLFKRVDISPLGAAAFAGSTLPINA 217 ********************************************************************* PP TIGR00838 208 ellaelLgFdavvensldavsdRDfiiEllsaaallmvhlsrlaEelilfsseEfgfvelsdevssgss 276 +++ elL F+++ +ns davsdRD iiE++sa++++m+hlsr++E+lil+ss+Ef+++e+sd++s+gss lcl|NCBI__GCF_000020545.1:WP_012474743.1 218 KRSMELLDFSDLFQNSTDAVSDRDIIIEFISACSIIMMHLSRFCEDLILWSSSEFNYIEISDAFSTGSS 286 ********************************************************************* PP TIGR00838 277 imPqKKnpDvaEliRgktgrviGnltglltilKalPlaYnkDlqEdkealfdalktveellevvtgllk 345 imPqKKn+D+aEl+Rgktgrv+Gnl+++lti+K+lPl+Yn+D+qEdk++lfd+ + ++++++t +l+ lcl|NCBI__GCF_000020545.1:WP_012474743.1 287 IMPQKKNADIAELVRGKTGRVYGNLMSILTIMKSLPLSYNRDMQEDKPPLFDSAGSTVSSVRIFTKMLA 355 ********************************************************************* PP TIGR00838 346 elkvnkerleeaakknfalatdlAdylvrkGvPFReaheivGevvakaiekGkkleeltleelqklsek 414 + ++n++rl+ ++ ++ +lat++A+ylvrk +PFReah+i+G++v +aiek ++l +++lee++ + lcl|NCBI__GCF_000020545.1:WP_012474743.1 356 HTRLNEKRLKTLTGEDLSLATEIAEYLVRKNLPFREAHRITGKIVSHAIEKRTSLPKISLEEYRGFCDL 424 ********************************************************************* PP TIGR00838 415 leedvlevldleeavekrdakGGtakeevekaieeakael 454 ++ed++++l++e +++ ++++G t++++ve++i a+ +l lcl|NCBI__GCF_000020545.1:WP_012474743.1 425 FNEDLYDALTPEASINSKHSHGSTSFASVEQQITIARGKL 464 *********************************9988765 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (455 nodes) Target sequences: 1 (466 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 8.63 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory