Alignments for a candidate for hisN in Prosthecochloris aestuarii DSM 271

GapMind for Amino acid biosynthesis

Alignments for a candidate for hisN in Prosthecochloris aestuarii DSM 271

Align Histidinol-phosphatase; HolPase; EC 3.1.3.15; Histidinol-phosphate phosphatase (uncharacterized)
to candidate WP_012506095.1 PAES_RS07710 histidinol-phosphatase

Query= curated2:P56160
         (259 letters)



>NCBI__GCF_000020625.1:WP_012506095.1
          Length = 253

 Score =  383 bits (983), Expect = e-111
 Identities = 186/252 (73%), Positives = 214/252 (84%), Gaps = 1/252 (0%)

Query: 1   MTPDLQLALELAEKAGKLTLDYFGRRSLQVFSKRDDTPVTEADRNAEELIRQGISAKFPD 60
           MT DLQLA+ELA++AGKLTL YF  +SL++  KRDDTPVTEADR AE+LIR+GI A++P+
Sbjct: 1   MTDDLQLAIELADEAGKLTLTYFQNKSLKIDEKRDDTPVTEADRKAEQLIRKGIEARYPE 60

Query: 61  DGLFGEEFDEHPSGNGRRWIIDPIDGTRSFIHGVPLYGVMIALEVEGAMQLGVINFPALG 120
           DG+FGEEF E  + NGRRWI+DPIDGTRSFIHGVPLYGVMIALEV  AMQLGVI FPAL 
Sbjct: 61  DGIFGEEFGEKIAVNGRRWILDPIDGTRSFIHGVPLYGVMIALEVNRAMQLGVIFFPALQ 120

Query: 121 ELYQAERGSGAFMNGSPVQVSAIAENSASTVVFTEKEYLLDPPSNHPVDQLRIDAGLVRG 180
           E+Y A+ G GAFMNG P++VS I +   +TVV+TEKEYLLDPPS+HPVD+LR DAGLVRG
Sbjct: 121 EMYYAQSGCGAFMNGEPLKVSEIGDTREATVVYTEKEYLLDPPSDHPVDRLRYDAGLVRG 180

Query: 181 WGDCYGHMLVASGRAEVAVDKIMSPWDCAAVIPIVEEAGGCCFDYRGRQSIIDGEGLVSA 240
           WGDCYGHMLVASGRAEVAVDK+MSPWDCAAVIP+V EAGGCCFDY  R +I  GEGLVSA
Sbjct: 181 WGDCYGHMLVASGRAEVAVDKVMSPWDCAAVIPVVTEAGGCCFDYNARTTIY-GEGLVSA 239

Query: 241 NNAMGRNLIAAI 252
           N  +G  L+  I
Sbjct: 240 NRKIGDELVRDI 251


Lambda     K      H
   0.319    0.138    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 288
Number of extensions: 7
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 259
Length of database: 253
Length adjustment: 24
Effective length of query: 235
Effective length of database: 229
Effective search space:    53815
Effective search space used:    53815
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

Align candidate WP_012506095.1 PAES_RS07710 (histidinol-phosphatase)
to HMM TIGR02067 (hisN: histidinol-phosphatase (EC 3.1.3.15))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR02067.hmm
# target sequence database:        /tmp/gapView.38317.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR02067  [M=252]
Accession:   TIGR02067
Description: his_9_HisN: histidinol-phosphatase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
    2.8e-90  288.2   0.0    3.2e-90  288.0   0.0    1.0  1  NCBI__GCF_000020625.1:WP_012506095.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000020625.1:WP_012506095.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  288.0   0.0   3.2e-90   3.2e-90       2     248 ..       5     248 ..       4     252 .. 0.97

  Alignments for each domain:
  == domain 1  score: 288.0 bits;  conditional E-value: 3.2e-90
                             TIGR02067   2 lalalelaeaageailkyfrasdlkvdkksdktpVteADraaEeaireliaakfPddgilGEEfgeeeedaey 74 
                                           l+la ela+ ag+ +l+yf++++lk d+k+d+tpVteADr+aE++ir+ i+a++P+dgi+GEEfge+   +++
  NCBI__GCF_000020625.1:WP_012506095.1   5 LQLAIELADEAGKLTLTYFQNKSLKIDEKRDDTPVTEADRKAEQLIRKGIEARYPEDGIFGEEFGEKIAVNGR 77 
                                           68999******************************************************************** PP

                             TIGR02067  75 vWvlDPiDGTksFirGvPvwgtLiaLlekgkpvlGvisqPalgerfvaakgegallnggerelrvsevaklsd 147
                                           +W+lDPiDGT+sFi+GvP++g++iaL+ + +++lGvi +Pal+e+++a++g ga++ng  ++l+vse+ +  +
  NCBI__GCF_000020625.1:WP_012506095.1  78 RWILDPIDGTRSFIHGVPLYGVMIALEVNRAMQLGVIFFPALQEMYYAQSGCGAFMNG--EPLKVSEIGDTRE 148
                                           *********************************************************7..8************ PP

                             TIGR02067 148 AvlvttspkaledeeereafeklrrkarltryggdcyayalvAsGrvdlvveaelspyDiaalipiieeAggv 220
                                           A++v+t++++l d+ + + +++lr +a l+r++gdcy+++lvAsGr++++v++++sp+D+aa+ip+++eAgg+
  NCBI__GCF_000020625.1:WP_012506095.1 149 ATVVYTEKEYLLDPPSDHPVDRLRYDAGLVRGWGDCYGHMLVASGRAEVAVDKVMSPWDCAAVIPVVTEAGGC 221
                                           ************************************************************************* PP

                             TIGR02067 221 itdwkGkeaeeggeavaaanaalhdevl 248
                                           ++d++ +++  g e++++an+++ de++
  NCBI__GCF_000020625.1:WP_012506095.1 222 CFDYNARTTIYG-EGLVSANRKIGDELV 248
                                           *****9887765.5569*****999876 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (252 nodes)
Target sequences:                          1  (253 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 12.10
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis