Align Phospho-2-dehydro-3-deoxyheptonate aldolase 2, chloroplastic; 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase 2; DAHP synthase 2; PhDAHP2; Phospho-2-keto-3-deoxyheptonate aldolase 2; EC 2.5.1.54 (characterized)
to candidate WP_012589176.1 MSIL_RS00640 3-deoxy-7-phosphoheptulonate synthase class II
Query= SwissProt::A0A067XGX8 (512 letters) >NCBI__GCF_000021745.1:WP_012589176.1 Length = 463 Score = 535 bits (1378), Expect = e-156 Identities = 264/451 (58%), Positives = 325/451 (72%), Gaps = 1/451 (0%) Query: 58 STTKWNLESWKTKPAFQLPDYPDKVELESVLKTLSTYPPIVFAGEARNLEEKLGEAALGN 117 S W+ SW+ KP Q P Y D L V + L+ +PP+VFAGEAR L+ LG+ A G Sbjct: 2 SVESWSPSSWRAKPIEQSPVYSDAAALADVERQLAGFPPLVFAGEARKLKRMLGKVANGE 61 Query: 118 AFLLQGGDCAESFKEFSANNIRDTFRVMLQMGVVLMFGGQMPVIKVGRMAGQFAKPRSDP 177 AFLLQGGDCAESF E SA+NIRD FRV LQM VV F +PV+KVGR+AGQFAKPRS P Sbjct: 62 AFLLQGGDCAESFAEHSADNIRDFFRVFLQMAVVETFAAALPVVKVGRIAGQFAKPRSAP 121 Query: 178 FEEKDGVKLPSYRGDNVNGDAFDEKSRIPDPHRMVRAYTQSVATLNLLRAFASGGYAAMQ 237 E GV LPSYRGD VN AF+ +R+PDP R + AY Q+ ATLNLLRAFA+GGYA ++ Sbjct: 122 NETVGGVSLPSYRGDIVNDIAFEASARVPDPARQLMAYRQAAATLNLLRAFATGGYANLE 181 Query: 238 RVNQWNLDFTDQSEQGDRYRELAHRVDEAMGFMTAAGLTVD-HTIMTTTDFWTSHECLLL 296 +QW L F S Q RY+ELA + + +GFM A GL + H + TDF+TSHE LLL Sbjct: 182 NAHQWMLGFIKDSPQSARYQELADHITQTLGFMRAIGLDPESHQELRQTDFYTSHEALLL 241 Query: 297 PYEQALTREDSTSGLYYDCSAHMIWVGERTRQLDGAHVEFLRGIANPLGIKVSHKMDPDE 356 +E+ALTR DST+G YY S HMIW+G+RTRQ AH+E+ RG+ NP+G+K ++PDE Sbjct: 242 GFEEALTRVDSTTGDYYATSGHMIWIGDRTRQPGHAHIEYARGVKNPIGLKCGPTLNPDE 301 Query: 357 LVKLIDILNPQNKPGRITVITRMGADNMRVKLPHLIRAVRGAGQIVTWVSDPMHGNTTKA 416 L++LIDILNP N+ GR+T+I R GAD + LP LIRAV+ G+ V W DPMHGNT KA Sbjct: 302 LIRLIDILNPDNEAGRLTLICRFGADKVEASLPTLIRAVQQEGRSVVWSCDPMHGNTVKA 361 Query: 417 PCGLKTRSFDSIRAELRAFFDVHEQEGSYPGGVHLEMTGQNVTECVGGSRTITYNDLSSR 476 G KTR FD I +E+R+FF VH+ EG+YPGGVHLEMTG+NVTEC GG+R I+ DL R Sbjct: 362 ASGYKTRPFDKIMSEIRSFFAVHQGEGTYPGGVHLEMTGKNVTECTGGARAISDADLHDR 421 Query: 477 YHTHCDPRLNASQALELAFAIAERLRRRRLG 507 YHT+CDPRLNA QA+E+AF IAE L+ R+G Sbjct: 422 YHTYCDPRLNAEQAIEVAFLIAELLKTGRMG 452 Lambda K H 0.319 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 680 Number of extensions: 26 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 512 Length of database: 463 Length adjustment: 34 Effective length of query: 478 Effective length of database: 429 Effective search space: 205062 Effective search space used: 205062 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
Align candidate WP_012589176.1 MSIL_RS00640 (3-deoxy-7-phosphoheptulonate synthase class II)
to HMM TIGR01358 (3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01358.hmm # target sequence database: /tmp/gapView.10185.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01358 [M=443] Accession: TIGR01358 Description: DAHP_synth_II: 3-deoxy-7-phosphoheptulonate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 9.3e-215 699.1 0.0 1.1e-214 698.9 0.0 1.0 1 lcl|NCBI__GCF_000021745.1:WP_012589176.1 MSIL_RS00640 3-deoxy-7-phosphohe Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000021745.1:WP_012589176.1 MSIL_RS00640 3-deoxy-7-phosphoheptulonate synthase class II # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 698.9 0.0 1.1e-214 1.1e-214 1 441 [. 6 448 .. 6 450 .. 0.99 Alignments for each domain: == domain 1 score: 698.9 bits; conditional E-value: 1.1e-214 TIGR01358 1 wsleswrskpaaqlPeyPdaealeavldtleslPPlvlageilklkeklaevakGeafllqgGdcaesf 69 ws++swr+kp q P y da+al v+ +l+ +PPlv+age++klk+ l++va+GeafllqgGdcaesf lcl|NCBI__GCF_000021745.1:WP_012589176.1 6 WSPSSWRAKPIEQSPVYSDAAALADVERQLAGFPPLVFAGEARKLKRMLGKVANGEAFLLQGGDCAESF 74 89******************************************************************* PP TIGR01358 70 keveadnirdklrvllqmavvltygaslPvvkvgriaGqyakPrsepieakdgvtlpsyrGdvingaaf 138 e +adnird +rv+lqmavv t++a lPvvkvgriaGq+akPrs+p e+ +gv lpsyrGd++n af lcl|NCBI__GCF_000021745.1:WP_012589176.1 75 AEHSADNIRDFFRVFLQMAVVETFAAALPVVKVGRIAGQFAKPRSAPNETVGGVSLPSYRGDIVNDIAF 143 ********************************************************************* PP TIGR01358 139 deaarvpdperlvrayaksaatlnllraltsgGyadlkkvheWnlefvkkspagaryeklaeeidealr 207 ++ arvpdp r + ay ++aatlnllra+++gGya+l++ h+W l f+k+sp++ary++la++i+++l lcl|NCBI__GCF_000021745.1:WP_012589176.1 144 EASARVPDPARQLMAYRQAAATLNLLRAFATGGYANLENAHQWMLGFIKDSPQSARYQELADHITQTLG 212 ********************************************************************* PP TIGR01358 208 fmsavgvaeae..alkrvelytsheallldyeealtrvdskegqlfdlsahllWiGertrqldgahvef 274 fm+a+g+++++ +l+++++ytshealll +eealtrvds++g+++ +s+h++WiG+rtrq +ah+e+ lcl|NCBI__GCF_000021745.1:WP_012589176.1 213 FMRAIGLDPEShqELRQTDFYTSHEALLLGFEEALTRVDSTTGDYYATSGHMIWIGDRTRQPGHAHIEY 281 ********987789******************************************************* PP TIGR01358 275 lrgvknPigikvgpsmeadellklievldPenePGrltlisrlGaekiaeklPelleavkaaGravvWv 343 +rgvknPig+k+gp++++del++li++l+P+ne Grltli r+Ga+k+ lP l++av+++Gr+vvW lcl|NCBI__GCF_000021745.1:WP_012589176.1 282 ARGVKNPIGLKCGPTLNPDELIRLIDILNPDNEAGRLTLICRFGADKVEASLPTLIRAVQQEGRSVVWS 350 ********************************************************************* PP TIGR01358 344 tdpmhGntleaasGyktrrfddilsevkeffevhkaeGthpGGvhleltGedvteclGGareitetdla 412 +dpmhGnt++aasGyktr fd+i+se+++ff+vh+ eGt+pGGvhle+tG++vtec+GGar+i++ dl+ lcl|NCBI__GCF_000021745.1:WP_012589176.1 351 CDPMHGNTVKAASGYKTRPFDKIMSEIRSFFAVHQGEGTYPGGVHLEMTGKNVTECTGGARAISDADLH 419 ********************************************************************* PP TIGR01358 413 sryetacdPrlnaeqslelaflvaeklre 441 +ry+t cdPrlnaeq++e+afl+ae l+ lcl|NCBI__GCF_000021745.1:WP_012589176.1 420 DRYHTYCDPRLNAEQAIEVAFLIAELLKT 448 *************************9986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (443 nodes) Target sequences: 1 (463 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01 # Mc/sec: 11.37 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory