Alignments for a candidate for glnA in Methylocella silvestris BL2

GapMind for Amino acid biosynthesis

Alignments for a candidate for glnA in Methylocella silvestris BL2

Align glutamate--ammonia ligase; EC 6.3.1.2 (characterized)
to candidate WP_012589471.1 MSIL_RS02165 type I glutamate--ammonia ligase

Query= CharProtDB::CH_024014
         (469 letters)



>NCBI__GCF_000021745.1:WP_012589471.1
          Length = 469

 Score =  608 bits (1567), Expect = e-178
 Identities = 295/463 (63%), Positives = 354/463 (76%), Gaps = 1/463 (0%)

Query: 6   VLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFFEEGKMFDGSSIGGWKGINESD 65
           VL  + +++VK+VD RFTD +GK QHVT     ++ + F EG MFDGSSI GWK INESD
Sbjct: 7   VLKEIKDNDVKYVDFRFTDPRGKWQHVTFDISLIDEDIFAEGTMFDGSSIAGWKAINESD 66

Query: 66  MVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYDRDPRSIAKRAEDYLRSTGIADTV 125
           M+LMPD STAV+DPFFA  T++I CDILEP + + Y RDPR IAK+AE +L S+GI D+V
Sbjct: 67  MLLMPDPSTAVMDPFFAAPTMVIVCDILEPNSNEPYSRDPRGIAKKAEAFLVSSGIGDSV 126

Query: 126 LFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWNSSTQYEGGNKGHRPAVKGGYFPVPP 185
             GPE EFF+FDD+R+ +    +   +D  E   NS T YEGGN GHR   KGGYFPVPP
Sbjct: 127 FVGPEAEFFVFDDVRYRADPYNTGFVLDSAELPSNSDTPYEGGNLGHRIRTKGGYFPVPP 186

Query: 186 VDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAGQNEVATRFNTMTKKADEIQIYKYVVHN 245
            DSAQD+R EM   M  MG  VE HHHEVA+A Q+E+  +F  +T  AD +Q+YKY +H 
Sbjct: 187 QDSAQDMRGEMLAAMAAMGAKVEKHHHEVASA-QHELGLKFGPLTTMADHLQVYKYCIHQ 245

Query: 246 VAHRFGKTATFMPKPMFGDNGSGMHCHMSLSKNGVNLFAGDKYAGLSEQALYYIGGVIKH 305
           VA  +GKTATFMPKP+FGDNGSGMH H S+ K G  LFAGDKYA LS++ LYYIGG+I+H
Sbjct: 246 VAQSYGKTATFMPKPVFGDNGSGMHVHQSIWKGGKPLFAGDKYADLSQECLYYIGGIIRH 305

Query: 306 AKAINALANPTTNSYKRLVPGYEAPVMLAYSARNRSASIRIPVVSSPKARRIEVRFPDPA 365
           A+A+NA  NP+TNSYKRLVPGYEAPV+LAYSARNRSAS RIP  S+PKA+R+EVRFPDP 
Sbjct: 306 ARALNAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPTASNPKAKRVEVRFPDPM 365

Query: 366 ANPYLCFAALLMAGLDGIKNKIHPGEAMDKNLYDLPPEEAKEIPQVAGSLEEALNELDLD 425
           ANPYL F+A+LMAGLDGI NKI PG AMDK+LYDLPP E K+IP V GSL EAL  LD D
Sbjct: 366 ANPYLAFSAMLMAGLDGIINKIDPGPAMDKDLYDLPPRELKKIPTVCGSLREALANLDKD 425

Query: 426 REFLKAGGVFTDEAIDAYIALRREEDDRVRMTPHPVEFELYYS 468
           R FLKAGGV+ D+ ID+YI L+  +  R  MTPHPVEF++YYS
Sbjct: 426 RAFLKAGGVYDDDFIDSYIELKMADVMRFEMTPHPVEFDMYYS 468


Lambda     K      H
   0.318    0.136    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 751
Number of extensions: 29
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 469
Length of database: 469
Length adjustment: 33
Effective length of query: 436
Effective length of database: 436
Effective search space:   190096
Effective search space used:   190096
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

Align candidate WP_012589471.1 MSIL_RS02165 (type I glutamate--ammonia ligase)
to HMM TIGR00653 (glnA: glutamine synthetase, type I (EC 6.3.1.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00653.hmm
# target sequence database:        /tmp/gapView.5499.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00653  [M=462]
Accession:   TIGR00653
Description: GlnA: glutamine synthetase, type I
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.8e-198  645.8   0.0   2.1e-198  645.6   0.0    1.0  1  lcl|NCBI__GCF_000021745.1:WP_012589471.1  MSIL_RS02165 type I glutamate--a


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000021745.1:WP_012589471.1  MSIL_RS02165 type I glutamate--ammonia ligase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  645.6   0.0  2.1e-198  2.1e-198       2     462 .]       6     468 ..       5     468 .. 0.99

  Alignments for each domain:
  == domain 1  score: 645.6 bits;  conditional E-value: 2.1e-198
                                 TIGR00653   2 evlkllkeenvkfvdlrfvDikGklkkveipvseleeealeegiaFDgssveGfksieesDlllkpdpe 70 
                                               +vlk +k+++vk+vd+rf+D +Gk+++v++ +s ++e+++ eg++FDgss+ G+k+i+esD+ll+pdp+
  lcl|NCBI__GCF_000021745.1:WP_012589471.1   6 DVLKEIKDNDVKYVDFRFTDPRGKWQHVTFDISLIDEDIFAEGTMFDGSSIAGWKAINESDMLLMPDPS 74 
                                               578999*************************************************************** PP

                                 TIGR00653  71 tlvivPfraekvlrvicdvyepvtkepyerdpRsiakraeeelk.tklgdevyfGpEaEFflfdkvefk 138
                                               t+v++Pf a+++++++cd+ ep  +epy+rdpR iak+ae  l  +++gd+v++GpEaEFf+fd+v+ +
  lcl|NCBI__GCF_000021745.1:WP_012589471.1  75 TAVMDPFFAAPTMVIVCDILEPNSNEPYSRDPRGIAKKAEAFLVsSGIGDSVFVGPEAEFFVFDDVRYR 143
                                               *******************************************999*********************** PP

                                 TIGR00653 139 easnssflevdseegewn..reveegnkgykikkkggYfdvepvDkakdirrelvlaleelglevevsH 205
                                                   ++ + +ds e   n  + +e+gn g++i++kggYf+v+p+D+a+d+r e++ a++++g +ve++H
  lcl|NCBI__GCF_000021745.1:WP_012589471.1 144 ADPYNTGFVLDSAELPSNsdTPYEGGNLGHRIRTKGGYFPVPPQDSAQDMRGEMLAAMAAMGAKVEKHH 212
                                               9999999*****99888866888999******************************************* PP

                                 TIGR00653 206 HEvataqaEidikfdklvkaaDeivlyKyvvknvakkhGktatFmpKplfgdngsGmHvhlslwkdgen 274
                                               HEva+aq+E+++kf  l+++aD++++yKy +++va+ +GktatFmpKp+fgdngsGmHvh+s+wk g++
  lcl|NCBI__GCF_000021745.1:WP_012589471.1 213 HEVASAQHELGLKFGPLTTMADHLQVYKYCIHQVAQSYGKTATFMPKPVFGDNGSGMHVHQSIWKGGKP 281
                                               ********************************************************************* PP

                                 TIGR00653 275 lfageegyagLsetalyyigGilkHakalaAltnptvnsYkRLvpGyEAPvylaysaknRsaliRiPas 343
                                               lfag++ ya+Ls+++lyyigGi++Ha+al+A+tnp++nsYkRLvpGyEAPv laysa+nRsa+ RiP++
  lcl|NCBI__GCF_000021745.1:WP_012589471.1 282 LFAGDK-YADLSQECLYYIGGIIRHARALNAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPTA 349
                                               ******.************************************************************** PP

                                 TIGR00653 344 anpkakRiEvRspDpsanpYLafaallmAgldGiknkidpgepldknlyelseeelkelGieqlpesLe 412
                                               +npkakR+EvR+pDp anpYLaf+a+lmAgldGi nkidpg ++dk+ly+l++ elk+  i+++  sL+
  lcl|NCBI__GCF_000021745.1:WP_012589471.1 350 SNPKAKRVEVRFPDPMANPYLAFSAMLMAGLDGIINKIDPGPAMDKDLYDLPPRELKK--IPTVCGSLR 416
                                               **********************************************************..********* PP

                                 TIGR00653 413 ealdelesdk..evlkevlgeelieafielkrkEveelrlkvhpvElekyld 462
                                               eal +l++d+  +++++v+ +++i+++ielk+++v ++++++hpvE+ +y++
  lcl|NCBI__GCF_000021745.1:WP_012589471.1 417 EALANLDKDRafLKAGGVYDDDFIDSYIELKMADVMRFEMTPHPVEFDMYYS 468
                                               **********999999**********************************95 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (462 nodes)
Target sequences:                          1  (469 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02
# Mc/sec: 10.26
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis