Alignments for a candidate for dapD in Methylocella silvestris BL2

GapMind for Amino acid biosynthesis

Alignments for a candidate for dapD in Methylocella silvestris BL2

Align 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (characterized)
to candidate WP_012590244.1 MSIL_RS06200 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase

Query= CharProtDB::CH_024869
         (274 letters)



>NCBI__GCF_000021745.1:WP_012590244.1
          Length = 285

 Score =  327 bits (837), Expect = 2e-94
 Identities = 180/280 (64%), Positives = 205/280 (73%), Gaps = 13/280 (4%)

Query: 4   LQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALRVAEKIDGQ-----WVTHQW 58
           L+ IIE AFE RA I       V R+AV+  + LLD+G LRVAEKI G+     W  HQW
Sbjct: 6   LETIIEAAFEDRANINAQTQGDV-RKAVDAALHLLDAGKLRVAEKIAGESGPQSWRVHQW 64

Query: 59  LKKAVLLSFRINDNQVIEGAE--SRYFDKVPMKFADYDEARFQKEGFRVVPPAAVRQGAF 116
           LKKAVLLSFR+ND   I G    + ++DKVP KFA +        GFR VP   VR+ A+
Sbjct: 65  LKKAVLLSFRLNDMGEIPGGPGGATWWDKVPSKFAGWGAKEHAAAGFRSVPSCVVRRSAY 124

Query: 117 IARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPLQANPT 176
           IA   VLMPS+VN+GAYVD+ TMVDTW TVGSCAQIGKNVHLSGGVGIGGVLEPLQANPT
Sbjct: 125 IAPGVVLMPSFVNLGAYVDQNTMVDTWVTVGSCAQIGKNVHLSGGVGIGGVLEPLQANPT 184

Query: 177 IIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRETGEIHYGRVPAGSVVVSG 236
           IIED+CFIGARSE+VEGVIV EG+V+SMG +I  ST+I DR TG+IH G VP  SVVVSG
Sbjct: 185 IIEDDCFIGARSEIVEGVIVGEGAVVSMGTFISASTKIIDRATGKIHIGYVPPFSVVVSG 244

Query: 237 NLPSK---DGK--YSLYCAVIVKKVDAKTRGKVGINELLR 271
           NLP K   DG    SLYCAVIVK VDA+TR K  IN+LLR
Sbjct: 245 NLPGKPLPDGSPGPSLYCAVIVKTVDAQTRAKTAINDLLR 284


Lambda     K      H
   0.318    0.136    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 278
Number of extensions: 12
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 274
Length of database: 285
Length adjustment: 26
Effective length of query: 248
Effective length of database: 259
Effective search space:    64232
Effective search space used:    64232
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

Align candidate WP_012590244.1 MSIL_RS06200 (2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase)
to HMM TIGR00965 (dapD: 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00965.hmm
# target sequence database:        /tmp/gapView.2980.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00965  [M=271]
Accession:   TIGR00965
Description: dapD: 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.6e-122  394.2   0.1   1.8e-122  394.0   0.1    1.0  1  lcl|NCBI__GCF_000021745.1:WP_012590244.1  MSIL_RS06200 2,3,4,5-tetrahydrop


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000021745.1:WP_012590244.1  MSIL_RS06200 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransfe
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  394.0   0.1  1.8e-122  1.8e-122       2     269 ..       6     284 ..       5     285 .] 0.94

  Alignments for each domain:
  == domain 1  score: 394.0 bits;  conditional E-value: 1.8e-122
                                 TIGR00965   2 lqkiietaferraeilpasklikvkeavnesiasldsgalrvaekldgq.....wkvnewvkkavllsf 65 
                                               l  iie afe ra+i   +++ +v++av  ++  ld g+lrvaek++g+     w+v++w+kkavllsf
  lcl|NCBI__GCF_000021745.1:WP_012590244.1   6 LETIIEAAFEDRANINA-QTQGDVRKAVDAALHLLDAGKLRVAEKIAGEsgpqsWRVHQWLKKAVLLSF 73 
                                               6789**********975.56789***********************76444555*************** PP

                                 TIGR00965  66 ritdnqvlnd..avnkyfdkvatkfadydedefkeaglrkvpgavvrrgafiaknvvlmpsyvnigayv 132
                                               r++d   + +  + + ++dkv++kfa +  +e   ag+r vp+ vvrr a+ia  vvlmps+vn+gayv
  lcl|NCBI__GCF_000021745.1:WP_012590244.1  74 RLNDMGEIPGgpGGATWWDKVPSKFAGWGAKEHAAAGFRSVPSCVVRRSAYIAPGVVLMPSFVNLGAYV 142
                                               ****988876224689***************************************************** PP

                                 TIGR00965 133 degtmvdtwatvgscaqigknvhlsggvgiggvleplqakpviiedncfigarseivegviveegsvis 201
                                               d+ tmvdtw tvgscaqigknvhlsggvgiggvleplqa+p+iied+cfigarseivegviv eg+v+s
  lcl|NCBI__GCF_000021745.1:WP_012590244.1 143 DQNTMVDTWVTVGSCAQIGKNVHLSGGVGIGGVLEPLQANPTIIEDDCFIGARSEIVEGVIVGEGAVVS 211
                                               ********************************************************************* PP

                                 TIGR00965 202 mgvfigqstkivdretgeiiygrvpagsvvvsgslpsk...dgk..kslycavivkkvdaktrgkvsin 265
                                               mg fi  stki+dr tg+i+ g vp  svvvsg+lp k   dg+  +slycavivk vda+tr+k++in
  lcl|NCBI__GCF_000021745.1:WP_012590244.1 212 MGTFISASTKIIDRATGKIHIGYVPPFSVVVSGNLPGKplpDGSpgPSLYCAVIVKTVDAQTRAKTAIN 280
                                               ***********************************9864558873358********************* PP

                                 TIGR00965 266 ellr 269
                                               +llr
  lcl|NCBI__GCF_000021745.1:WP_012590244.1 281 DLLR 284
                                               ***9 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (271 nodes)
Target sequences:                          1  (285 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.06
# Mc/sec: 1.24
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis