Align threonine synthase (EC 4.2.3.1) (characterized)
to candidate WP_012590290.1 MSIL_RS06420 threonine synthase
Query= BRENDA::Q8YFS0 (463 letters) >NCBI__GCF_000021745.1:WP_012590290.1 Length = 473 Score = 545 bits (1403), Expect = e-159 Identities = 277/461 (60%), Positives = 341/461 (73%), Gaps = 3/461 (0%) Query: 1 MKYVSTRGEAPVLGFSDALLAGLARDGGLYLPQEYPQFTAEQIRALRGKSYVEVALAVLT 60 MKYVSTRGEA L F+ LLAGLA DGGLYLP+ YP+ AE+I + GKSY +V AV++ Sbjct: 1 MKYVSTRGEAAPLSFTQTLLAGLAPDGGLYLPESYPRLDAERIASFSGKSYTDVTEAVIS 60 Query: 61 PFTGGEIPAADFERMVREAYGTFRHDAVCPLVQTDANEFVLELFHGPTLAFKDVAMQLLA 120 PF GGEI A M+ +AY FRH AV PLVQ D N F LELFHGPTLAFKD+AMQLLA Sbjct: 61 PFIGGEIVPAALRAMIDDAYAGFRHPAVAPLVQLDDNLFTLELFHGPTLAFKDLAMQLLA 120 Query: 121 RMMDYVLAQRGERATIVGATSGDTGGAAIEAFGGRDNTDIFILFPNGRVSPVQQRQMTSS 180 R++D+ L RG+RAT+VGATSGDTG AAIEAF G ++FIL+P+ RVS VQ+RQMT++ Sbjct: 121 RLVDHALKARGQRATVVGATSGDTGAAAIEAFSGLSQVEVFILYPHQRVSEVQRRQMTTT 180 Query: 181 GFSNVHALSIEGNFDDCQNLVKGMFNDLEFCDALSLSGVNSINWARIMPQVVYYFTAALS 240 G NVHA+++EG+FDD Q+ VK +FN+ ++L+L GVNSINWARI+ Q VYYFTAA++ Sbjct: 181 GAPNVHAIAVEGSFDDAQSAVKALFNNRPLRESLNLCGVNSINWARILAQTVYYFTAAVA 240 Query: 241 LGAPDRAVSFTVPTGNFGDIFAGYVAKRMGLPIEQLIIATNDNDILSRTLESGAYEMRGV 300 LGAP R VSF PTGNFGDIFAG++AKRMGLPI++L+IA N NDIL R LESGAY + V Sbjct: 241 LGAPHRPVSFVTPTGNFGDIFAGWIAKRMGLPIDRLVIAANSNDILPRALESGAYAVGEV 300 Query: 301 AQTTSPSMDIQISSNFERLLFEAHGRDAAAVRGLMQGLKQSGGFTISEKPLSAIRSEFSA 360 T SPSMDIQ+SSNFERLLFEA GRD AAVR M L QS GFTI PL AIR++F A Sbjct: 301 HATQSPSMDIQVSSNFERLLFEAQGRDGAAVRAWMAQLSQSRGFTIDPAPLKAIRADFDA 360 Query: 361 GRSTVDETAATIESVLSKDGYLLDPHSAIGVKVAREKAS---GTAPMVVLATAHPAKFPD 417 + ET A I + + GYL DPH+A+G+ AR + + P+VVL TAH AKFPD Sbjct: 361 FSVSEPETTAEIARLWRESGYLADPHTAVGLGAARREIATRGAATPLVVLGTAHAAKFPD 420 Query: 418 AVKAACGVEPQLPAWLCDLMQRKESFTVLHNELKIVEEYVR 458 A++AA GV P LP L L++R E F+++ N+ VE ++R Sbjct: 421 AIEAATGVRPALPPHLHGLLERAEQFSIIPNDQGAVERFIR 461 Lambda K H 0.320 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 599 Number of extensions: 25 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 463 Length of database: 473 Length adjustment: 33 Effective length of query: 430 Effective length of database: 440 Effective search space: 189200 Effective search space used: 189200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 51 (24.3 bits)
Align candidate WP_012590290.1 MSIL_RS06420 (threonine synthase)
to HMM TIGR00260 (thrC: threonine synthase (EC 4.2.3.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00260.hmm # target sequence database: /tmp/gapView.29097.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00260 [M=340] Accession: TIGR00260 Description: thrC: threonine synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1e-86 277.1 0.0 1.3e-86 276.8 0.0 1.0 1 lcl|NCBI__GCF_000021745.1:WP_012590290.1 MSIL_RS06420 threonine synthase Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000021745.1:WP_012590290.1 MSIL_RS06420 threonine synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 276.8 0.0 1.3e-86 1.3e-86 15 334 .. 72 428 .. 63 433 .. 0.91 Alignments for each domain: == domain 1 score: 276.8 bits; conditional E-value: 1.3e-86 TIGR00260 15 lvdlaegstelfrspklaeevga.enlyvkelfhgPtlaFKDrglqfvavlltkalelgne..tvlcAt 80 l +++ ++ fr p++a v++ nl+ +elfhgPtlaFKD+++q +a l+ +al++ + tv++At lcl|NCBI__GCF_000021745.1:WP_012590290.1 72 LRAMIDDAYAGFRHPAVAPLVQLdDNLFTLELFHGPTLAFKDLAMQLLARLVDHALKARGQraTVVGAT 140 66777888999*******9999999*****************************988655456****** PP TIGR00260 81 sGdtgaaaaealagkanvkvvvLyPkgkispv.keklvtalaenakvlaikGdFDdaqdlvkeifedke 148 sGdtgaaa+ea++g + v+v++LyP+ ++s v +++t+ a n++ +a++G FDdaq +vk++f++++ lcl|NCBI__GCF_000021745.1:WP_012590290.1 141 SGDTGAAAIEAFSGLSQVEVFILYPHQRVSEVqRRQMTTTGAPNVHAIAVEGSFDDAQSAVKALFNNRP 209 ********************************99********************************666 PP TIGR00260 149 ...klklnsvNsinparieaqktyafeiveqlgkespdkvvvpvpsgnfgailkGflekkelglpiekl 214 l+l vNsin+ari+aq +y+f ++ +lg v ++ p gnfg+i++G+ +k+++ lpi+ l lcl|NCBI__GCF_000021745.1:WP_012590290.1 210 lreSLNLCGVNSINWARILAQTVYYFTAAVALG-APHRPVSFVTPTGNFGDIFAGWIAKRMG-LPIDRL 276 666******************************.66667999999**********9999999.****** PP TIGR00260 215 aiaaegaadivrrflksgdlepkedkeTlstAmdignpsnverale.larrslgnledlke........ 274 iaa+++ di++r l+sg e +T s++mdi +sn+er+l+ + r ++ + + lcl|NCBI__GCF_000021745.1:WP_012590290.1 277 VIAANSN-DILPRALESGAYAVGEVHATQSPSMDIQVSSNFERLLFeAQGRDGAAVR---Awmaqlsqs 341 ******9.**********88888888******************9744444444444...34459999* PP TIGR00260 275 ....................svsdeeileaikklaeeegyllephtavavaalkklvekg..vs..... 316 svs+ e++ +i +l +e gyl++phtav++ a ++ ++ + lcl|NCBI__GCF_000021745.1:WP_012590290.1 342 rgftidpaplkairadfdafSVSEPETTAEIARLWRESGYLADPHTAVGLGAARREIATRgaATplvvl 410 ********************************************************9987761145667 PP TIGR00260 317 atadpaKFeevvealtgn 334 +ta+ aKF++++ea tg lcl|NCBI__GCF_000021745.1:WP_012590290.1 411 GTAHAAKFPDAIEAATGV 428 *************99985 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (340 nodes) Target sequences: 1 (473 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 10.22 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory