Alignments for a candidate for proC in Sulfurihydrogenibium azorense Az-Fu1

GapMind for Amino acid biosynthesis

Alignments for a candidate for proC in Sulfurihydrogenibium azorense Az-Fu1

Align pyrroline-5-carboxylate reductase (EC 1.5.1.2) (characterized)
to candidate WP_012674059.1 SULAZ_RS08005 pyrroline-5-carboxylate reductase

Query= BRENDA::G7KRM5
         (274 letters)



>NCBI__GCF_000021545.1:WP_012674059.1
          Length = 261

 Score =  182 bits (461), Expect = 8e-51
 Identities = 107/264 (40%), Positives = 161/264 (60%), Gaps = 5/264 (1%)

Query: 11  YTLGFIGAGKMAESIAKGAV-RSGVLSPSRIKTAIHSNPARRTAFESIGITVLSSNDDVV 69
           + +G IG G M E+I KG + ++G+ S S +   I  N  R+           S    VV
Sbjct: 2   FKVGVIGCGNMGEAIIKGLIEKAGINSTSIVVNDI--NKERQNYIVEKYNVAGSDIRKVV 59

Query: 70  RDSNVVVFSVKPQLLKDVVLKLKPLLTKDKLLVSVAAGIKMKDLQEWAGHERFIRVMPNT 129
             S +V   VKP+ L+D +  +K L  ++++L+SV AG+K++ ++        +R+MPNT
Sbjct: 60  NLSEIVFLVVKPKDLQDSLESIKDLFKENQILISVLAGVKIEKIKSIVEKPLVVRIMPNT 119

Query: 130 AATVGEAASVMSLGGAATEEDANLISQLFGSIGKIWKADDKYFDAITGLSGSGPAYIYLA 189
            A +GE    +S     TE+   +I  L G +G++++  ++  D ITGLSGSGPAY++  
Sbjct: 120 PALIGEGVIGVSFE-KDTEKKQEIIDLLKG-LGEVFEVKEELLDVITGLSGSGPAYVFTF 177

Query: 190 IEALADGGVAAGLPRDLALSLASQTVLGAASMATQSGKHPGQLKDDVTSPGGTTIAGVHE 249
           I+ALA GGV  GLP   AL +A+QTVLGAA +  ++G+HP  L+D V+SP GTTI G+HE
Sbjct: 178 IDALAQGGVKMGLPYQDALKIATQTVLGAAKLLKETGEHPAVLRDTVSSPAGTTIYGLHE 237

Query: 250 LEKAGFRGILMNAVVAAAKRSQEL 273
           LEK  F+  +++AV  A KRS+EL
Sbjct: 238 LEKKNFKDAVISAVETATKRSKEL 261


Lambda     K      H
   0.315    0.131    0.362 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 152
Number of extensions: 5
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 274
Length of database: 261
Length adjustment: 25
Effective length of query: 249
Effective length of database: 236
Effective search space:    58764
Effective search space used:    58764
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 47 (22.7 bits)

Align candidate WP_012674059.1 SULAZ_RS08005 (pyrroline-5-carboxylate reductase)
to HMM TIGR00112 (proC: pyrroline-5-carboxylate reductase (EC 1.5.1.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00112.hmm
# target sequence database:        /tmp/gapView.5564.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00112  [M=263]
Accession:   TIGR00112
Description: proC: pyrroline-5-carboxylate reductase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
      4e-91  291.3   0.8    4.5e-91  291.1   0.8    1.0  1  lcl|NCBI__GCF_000021545.1:WP_012674059.1  SULAZ_RS08005 pyrroline-5-carbox


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000021545.1:WP_012674059.1  SULAZ_RS08005 pyrroline-5-carboxylate reductase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  291.1   0.8   4.5e-91   4.5e-91       1     263 []       4     261 .]       4     261 .] 0.96

  Alignments for each domain:
  == domain 1  score: 291.1 bits;  conditional E-value: 4.5e-91
                                 TIGR00112   1 iaiiGaGnmgeallsgllkkgakakkeilvierseeklaalakelgvevtsdaeeavkeadvvllavKP 69 
                                               +++iG+Gnmgea+++gl++k+ +++++i+v + ++e+ ++ +++++v+ +   +++v+ +++v+l vKP
  lcl|NCBI__GCF_000021545.1:WP_012674059.1   4 VGVIGCGNMGEAIIKGLIEKAGINSTSIVVNDINKERQNYIVEKYNVAGSDI-RKVVNLSEIVFLVVKP 71 
                                               689********************99999999999***********9887666.7899************ PP

                                 TIGR00112  70 qdleevlaelkseektkeklliSilAGvtiekleqlleaekrvvRvmPNtaakvgagvtaiaassevse 138
                                               +dl++ l+++k+   +++++liS+lAGv+iek+++ +e+   vvR+mPNt+a +g+gv++++ ++++  
  lcl|NCBI__GCF_000021545.1:WP_012674059.1  72 KDLQDSLESIKD-LFKENQILISVLAGVKIEKIKSIVEK-PLVVRIMPNTPALIGEGVIGVSFEKDT-- 136
                                               ***********9.55589*******************85.88**********************654.. PP

                                 TIGR00112 139 eqkelveellkavGkvveveeklldavtalsGSgPAfvflliealadagvklGLpreeakelaaqtlkG 207
                                               e+k+++ +llk +G+v ev+e+lld +t+lsGSgPA+vf++i+ala++gvk+GLp + a+++a+qt+ G
  lcl|NCBI__GCF_000021545.1:WP_012674059.1 137 EKKQEIIDLLKGLGEVFEVKEELLDVITGLSGSGPAYVFTFIDALAQGGVKMGLPYQDALKIATQTVLG 205
                                               56789**************************************************************** PP

                                 TIGR00112 208 aaklleesgehpalLkdkVtsPgGtTiaglavLeekgvrsavieaveaavkrseeL 263
                                               aakll+e+gehpa+L+d V+sP+GtTi gl++Le+k++++avi ave a+krs+eL
  lcl|NCBI__GCF_000021545.1:WP_012674059.1 206 AAKLLKETGEHPAVLRDTVSSPAGTTIYGLHELEKKNFKDAVISAVETATKRSKEL 261
                                               ******************************************************98 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (263 nodes)
Target sequences:                          1  (261 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 5.81
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis