Align glutamate-5-semialdehyde dehydrogenase (EC 1.2.1.41) (characterized)
to candidate WP_012676561.1 PERMA_RS08340 gamma-glutamyl-phosphate reductase
Query= BRENDA::A7Y114 (420 letters) >NCBI__GCF_000021565.1:WP_012676561.1 Length = 445 Score = 384 bits (985), Expect = e-111 Identities = 201/418 (48%), Positives = 279/418 (66%), Gaps = 8/418 (1%) Query: 11 EAQAIEAKKASKSLMILSDKEKNDALHHIADVLERDFEAILAANEKDLRNGREQGFTEAF 70 E A EA+++ K L ++ KN L A++L E I AN+KDL +++G+++A Sbjct: 8 EKLAREARESQKFLRRVTTDIKNRVLLRTAELLIEKKEIIKEANDKDLEFAQKKGYSKAL 67 Query: 71 MDRLSLSQERIRDFAQGLRDVAELEDPTGKKLSDWTLENGLQVEKVTVPLGVIGMIYEAR 130 +DRL+L+++RI Q L+DVA L DP G+ +S WT NGL+V ++ VPLG + +IYEAR Sbjct: 68 LDRLTLNEKRINGMVQVLKDVASLPDPVGEIISMWTRPNGLRVGQMRVPLGTVMIIYEAR 127 Query: 131 PNVTVDATGLALKSGNAIVLKGGSSAINSNQAIVEVIHKALAETKIPQESVQFIASTDRA 190 PNVTV+A L +KS NA++LKGGS INSN+ +VE++ +A E+ P+ ++QF+ +TDR Sbjct: 128 PNVTVEAASLCIKSSNAVILKGGSETINSNRVLVEILRQAARESGFPERAIQFVDTTDRE 187 Query: 191 ATNQLFTMKEHVDVLIPRGGGKLIQAVVENATVPVLETGVGNCHIYIDKDADVEKAISIL 250 A N L T+ +++DV+IPRGG LI+AV E AT+PV++ G C++YID +AD+EKA++I Sbjct: 188 AVNHLLTLDQYIDVVIPRGGEGLIRAVAEKATMPVIKHYKGVCNLYIDDEADMEKALNIA 247 Query: 251 VNAKTDRPAVCNAAETLIVHKDWLAQHSDELISALKKENIHVHGDEHVLTIIPD------ 304 NAK +RP+VCNA E LIVHKD + E+ K + + DE L I+ D Sbjct: 248 YNAKVERPSVCNAIENLIVHKDIAEKFLPEIAYYYGKAGVEMRCDERALEILRDHPKAND 307 Query: 305 --AVPAGEDDWKNEYLSTDIAVKAVDDLVDAIAHIETYGTKHSEAIVTENQEAADKFLAI 362 VPA EDD+ E+L IAVK VD + AI I YG+ HSE+IVTEN +F+ Sbjct: 308 TEIVPAKEDDYYEEFLDLIIAVKVVDSIDQAIDFIHKYGSNHSESIVTENYTKGMRFINE 367 Query: 363 VDAAAIYHNASTRFTDGGALGFGAEIGISTQKLHARGPMGLPALTTVKFLMKGTGQIR 420 VD++A+Y NASTRFTDG G GAE+GIST K+H RGPMGL LT KF++ G GQIR Sbjct: 368 VDSSAVYINASTRFTDGNEFGLGAEMGISTDKIHVRGPMGLKELTIPKFIIFGDGQIR 425 Lambda K H 0.315 0.131 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 468 Number of extensions: 22 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 420 Length of database: 445 Length adjustment: 32 Effective length of query: 388 Effective length of database: 413 Effective search space: 160244 Effective search space used: 160244 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 51 (24.3 bits)
Align candidate WP_012676561.1 PERMA_RS08340 (gamma-glutamyl-phosphate reductase)
to HMM TIGR00407 (proA: glutamate-5-semialdehyde dehydrogenase (EC 1.2.1.41))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00407.hmm # target sequence database: /tmp/gapView.7248.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00407 [M=398] Accession: TIGR00407 Description: proA: glutamate-5-semialdehyde dehydrogenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.7e-153 496.0 0.7 4.5e-153 495.7 0.7 1.0 1 lcl|NCBI__GCF_000021565.1:WP_012676561.1 PERMA_RS08340 gamma-glutamyl-pho Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000021565.1:WP_012676561.1 PERMA_RS08340 gamma-glutamyl-phosphate reductase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 495.7 0.7 4.5e-153 4.5e-153 1 395 [. 14 412 .. 14 415 .. 0.96 Alignments for each domain: == domain 1 score: 495.7 bits; conditional E-value: 4.5e-153 TIGR00407 1 akeaalklaqlstaeknealskiadeLkaeaelilaanakdiaaakenGladalldrLlLteeklksia 69 a+e+ l ++t+ kn++l a+ L ++ e+i +an kd++ a+++G ++alldrL+L+e+++++++ lcl|NCBI__GCF_000021565.1:WP_012676561.1 14 ARESQKFLRRVTTDIKNRVLLRTAELLIEKKEIIKEANDKDLEFAQKKGYSKALLDRLTLNEKRINGMV 82 78888999************************************************************* PP TIGR00407 70 ddvkdvieLadPvGkvieareldeGLklervrvPlGvlgviyearPevivdvasLclktGnaviLkGgk 138 + +kdv++L+dPvG++i+ ++ +GL++ ++rvPlG + +iyearP+v+v++asLc+k+ naviLkGg+ lcl|NCBI__GCF_000021565.1:WP_012676561.1 83 QVLKDVASLPDPVGEIISMWTRPNGLRVGQMRVPLGTVMIIYEARPNVTVEAASLCIKSSNAVILKGGS 151 ********************************************************************* PP TIGR00407 139 eavrsnkalveviqdaleqtglpveavqliedpdreevkellkldeyvdlliPrGgnelvklikeesti 207 e+++sn++lve++++a +++g p a+q ++++dre v++ll+ld+y+d++iPrGg++l++ + e++t+ lcl|NCBI__GCF_000021565.1:WP_012676561.1 152 ETINSNRVLVEILRQAARESGFPERAIQFVDTTDREAVNHLLTLDQYIDVVIPRGGEGLIRAVAEKATM 220 ********************************************************************* PP TIGR00407 208 PvlehadGvChiyldesadlakakkvivdaktqrPstCnaietLLvnkaiaeefleeLekqleekgvel 276 Pv++h +GvC++y+d++ad++ka ++ +ak +rPs+Cnaie L+v+k+iae+fl+e++ ++gve+ lcl|NCBI__GCF_000021565.1:WP_012676561.1 221 PVIKHYKGVCNLYIDDEADMEKALNIAYNAKVERPSVCNAIENLIVHKDIAEKFLPEIAYYYGKAGVEM 289 ********************************************************************* PP TIGR00407 277 radalvlkllele.kate...aevskedfdkeflsldLsvkivedleeaiehirqygtkhsdailtedk 341 r d+ +l++l+ ka++ + ++++d+ +efl+l+++vk+v+++++ai+ i++yg++hs++i+te+ lcl|NCBI__GCF_000021565.1:WP_012676561.1 290 RCDERALEILRDHpKANDteiVPAKEDDYYEEFLDLIIAVKVVDSIDQAIDFIHKYGSNHSESIVTENY 358 ****99998754314444111446677****************************************** PP TIGR00407 342 knaekfvkevdsaavyvnastrfadGfrfGfGaevgistqklharGPvGLeaLv 395 ++ +f++evds+avy nastrf+dG +fG+Gae+gist+k+h rGP+GL+ L+ lcl|NCBI__GCF_000021565.1:WP_012676561.1 359 TKGMRFINEVDSSAVYINASTRFTDGNEFGLGAEMGISTDKIHVRGPMGLKELT 412 ***************************************************998 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (398 nodes) Target sequences: 1 (445 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 10.94 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory