Annotation: NCBI__GCF_000018545.1:WP_012708041.1
Length: 346 amino acids
Source: GCF_000018545.1 in NCBI
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|---|---|---|---|---|---|---|---|---|
| L-cysteine biosynthesis | cysK | hi | cysteine synthase (EC 2.5.1.47) (characterized) | 75% | 100% | 538.1 | cysteine-S-conjugate beta-lyase (EC 4.4.1.13) | 40% | 239.6 |
| L-cysteine biosynthesis | CBS | lo | Cystathionine beta-synthase; Beta-thionase; Hemoprotein H-450; Serine sulfhydrase; EC 4.2.1.22 (characterized) | 41% | 57% | 232.6 | cysteine synthase (EC 2.5.1.47) | 75% | 538.1 |
| L-cysteine biosynthesis | PSSH | lo | O-phosphoserine sulfhydrylase monomer (EC 2.5.1.47; EC 2.5.1.65) (characterized) | 38% | 100% | 193 | cysteine synthase (EC 2.5.1.47) | 75% | 538.1 |
| L-cysteine biosynthesis | Mt_cysM | lo | [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase (EC 2.5.1.113); O-phosphoserine sulfhydrylase (EC 2.5.1.65) (characterized) | 32% | 93% | 137.1 | cysteine synthase (EC 2.5.1.47) | 75% | 538.1 |
View WP_012708041.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
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MSVYPSVLEAIGNTPLIRLNAVSEATGCTILGKAEFLNPGQSVKDRAALWIIREAEKAGQ LRPGGVIVEGTAGNTGIGLAVVGSALGYRTVIVIPETQSQEKKDALRLLGAELVEVPAVP YKNPNNYVKISGRLAAELAKTEPNGAIWANQFDNVANREAHVQTTAPEIWRDTDGKVDGF ICAVGSGGTIAGVAEGLRAKNPAVKIGIADPEGAALYNYYAHGELKASGSSITEGIGQGR VTANLEGFTPDFAYQIPDSEAVPYVFDLIEKEGLCVGGSSGINIAGAVRLARELGPGHTI VTILCDYGNRYQSKLFNPDFLASKGLPVPAWLNKSSNITVPYEPIG
This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory